Ngwa SARS-CoV-2 Nchọpụta Nucleic Acid (Usoro nyocha Fluorescent PCR Multiplex)

Nkọwa ngwa:

 

PCR na-arụ ọrụ nke ọma:Enweghị mkpa maka ngwa mkpochapụ acid nucleic acid na Sistemu, nke emechara 96 ​​n'ime elekere 1 ma ọ bụ karịa.

 

◮ Ngwá ọrụ dị ala chọrọ yana iji mgbanwe dị ukwuu:Naanị sistemụ PCR Real-Time chọrọ.

 

LoD na mmetụta dị elu:Mmetụta nchọta nwere ike iru ọkwa kacha ala dị ka 500 nnomi/ml.

foregene strength


Nkọwa ngwaahịa

Mkpado ngwaahịa

Ezubere iji

Ngwa a na-eji teknụzụ RT PCR Real-time (rRT-PCR) maka nchọpụta qualitative nke SARS-CoV-2 nucleic acid n'ime mmadụ nasopharyngeal ma ọ bụ oropharyngeal swab sample.

Ụkpụrụ nke ule

Emebere primer na nyocha nyocha maka ịchọpụta usoro echekwara nke SARS-CoV-2 (ORF1ab gene na N gene).Ngwa ahụ nwere njikwa ime nke a na-eji enyocha ịdịmma ụdị.
Teknụzụ PCR dị adị na-eji mmeghachi omume polymerase chain (PCR) maka mmụba nke ebumnuche akọwapụtara
usoro na lekwasịrị anya nyocha kpọmkwem maka nchọpụta nke RNA agbagoro.Ejiri akara nyocha ndị ahụ
onye nta akụkọ fluorescent na ịcha akwa.

Nkọwa ngwa ngwa

Nkọwapụta:48 Rxns/kit, 96 Rxns/kit

◮ Nkọwapụta:96.72%

Mmetụta:97.92%

◮Ụdị ihe nlere anya
Nasopharyngeal Swab ma ọ bụ Oropharyngeal Swab
Ngwa ọdabara
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S

 

Ngwa

Mba.

Akụkụ

Ọnụ ego

Isi ihe

48 Rxn

96 Rxn

1 Ntọhapụ nke Nucleic acid 1.4ml / tube 2 ọkpọkọ 5.3 ml / karama 1 karama Surfactant
2 ihe nchekwa RNA 27 μl / tube 1 tube 53 μl / tube 1 tube Ihe mgbochi RNase
3 Ihe ngwọta mmeghachi omume SARS-CoV-2 800 μl / tube 1 tube 1600 μl / tube 1 tube Ndabere, nyocha, ihe nchekwa mmeghachi omume, dNTP
4 Ngwakọta enzyme SARS-CoV-2 80 μl / tube 1 tube 160 μl / tube 1 tube Hot mmalite Taq enzyme, M-MLV enzyme
5 SARS-CoV-2 ezigbo njikwa 100 μl / tube 1 tube 100 μl / tube 1 tube Plasmid recombinant nwere iberibe ebumnuche, RNA
6 SARS-CoV-2 njikwa adịghị mma 1200 μl / tube 1 tube 1200 μl / tube 1 tube Ihe nchekwa TE

Atụmatụ & uru

PCR ozugbo
Enweghị mkpa maka ngwa mkpochapụ acid nucleic acid na Sistemu, nke emechara 96 ​​n'ime elekere 1 ma ọ bụ karịa.
Achọrọ akụrụngwa dị ala yana ojiji a na-agbanwe agbanwe nke ukwuu
Naanị sistemụ PCR Real-Time chọrọ.
LoD na mmetụta dị elu
Mmetụta nchọta nwere ike iru ọkwa kacha ala dị ka 500 nnomi/ml.
amplification curve-RT-qPCR

Otu esi eji

Usoro 1: Direct PCR usoro

Usoro 2: Viral RNA Isolation + PCR

Usoro 1: Direct PCR usoro ijiIhe nnọchi anya mwepụta nlele  

 

Usoro nchọpụta

Operation Procedures

Procedures

Ụgbọ njem na Nchekwa Chain oyi

Ejiri ya site na ìhè ma chekwaa na -20 ± 5 ℃;

storage
storage2

Ndụ nchekwa

1 afọ

Ozi ịtụ

Ordering information

Vidiyo na-arụ ọrụ ngwa ngwa


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