Ngwa SARS-CoV-2 Nchọpụta Nucleic Acid (Usoro nyocha Fluorescent PCR Multiplex)
Nkọwa ngwa:
Nke a bụ kpọmkwem PCR:Enweghị mkpa maka ngwa mkpochapụ acid nucleic acid na Sistemu, nke emechara 96 n'ime elekere 1 ma ọ bụ karịa.
◮ Ngwa akụrụngwa dị ala yana ojiji nke ukwuu na-agbanwe agbanwe:Naanị sistemụ PCR Real-Time chọrọ.
LoD na mmetụta dị elu:Nchọpụta nchọta nwere ike iru ọkwa kacha ala dị ka 500 nnomi/ml.
Ezubere iji
Ngwa a na-eji teknụzụ RT PCR Real-time (rRT-PCR) maka nchọpụta qualitative nke SARS-CoV-2 nucleic acid n'ime mmadụ nasopharyngeal ma ọ bụ oropharyngeal swab sample.
Ụkpụrụ nke ule
Emebere primer na nyocha nyocha maka ịchọpụta usoro echekwara nke SARS-CoV-2 (ORF1ab gene na N gene).Ngwa ahụ nwere njikwa dị n'ime nke a na-eji enyocha ogo ihe atụ.
Teknụzụ PCR dị adị na-eji mmeghachi omume polymerase chain (PCR) maka mmụba nke ebumnuche akọwapụtara
usoro na lekwasịrị anya nyocha kpọmkwem maka nchọpụta nke RNA gbasiri ike.Ejiri akara nyocha ndị ahụ
onye nta akụkọ fluorescent na agba agba.
Nkọwa ngwa ngwa
◮Nkọwapụta:48 Rxns/kit, 96 Rxns/kit
◮ Nkọwapụta:96.72%
Mmetụta:97.92%
◮ Ụdị ihe atụ dị
Nasopharyngeal Swab ma ọ bụ Oropharyngeal Swab
◮Ngwa ngwa
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
Ngwa
| Mba. | Akụkụ | Ọnụ ego | Isi ihe | |||
| 48 Rxn | 96 Rxn | |||||
| 1 | Ihe ntọhapụ nke Nucleic acid | 1.4ml / tube | 2 ọkpọkọ | 5.3 ml / karama | 1 karama | Surfactant |
| 2 | ihe nchekwa RNA | 27 μl / tube | 1 tube | 53 μl / tube | 1 tube | Ihe mgbochi RNase |
| 3 | Ngwọta mmeghachi omume SARS-CoV-2 | 800 μl / tube | 1 tube | 1600 μl / tube | 1 tube | Ndabere, nyocha, ihe nchekwa mmeghachi omume, dNTP |
| 4 | Ngwakọta enzyme SARS-CoV-2 | 80 μl / tube | 1 tube | 160 μl / tube | 1 tube | Mmalite Taq Enzyme dị ọkụ, M-MLV enzyme |
| 5 | SARS-CoV-2 ezigbo njikwa | 100 μl / tube | 1 tube | 100 μl / tube | 1 tube | Plasmid recombinant nwere iberibe ebumnuche, RNA |
| 6 | SARS-CoV-2 njikwa adịghị mma | 1200 μl / tube | 1 tube | 1200 μl / tube | 1 tube | Ihe nchekwa TE |
Atụmatụ & uru
◮PCR ozugbo
Enweghị mkpa maka ngwa mkpochapụ acid nucleic acid na Sistemu, nke emechara 96 n'ime elekere 1 ma ọ bụ karịa.
◮Achọrọ akụrụngwa dị ala yana ojiji a na-agbanwe agbanwe
Naanị sistemụ PCR Real-Time chọrọ.
◮LoD na mmetụta dị elu
Nchọpụta nchọta nwere ike iru ọkwa kacha ala dị ka 500 nnomi/ml.
Otu esi eji
Usoro 1: Direct PCR usoro
Usoro 2: Viral RNA Isolation + PCR
Usoro 1: Direct PCR usoro ijiIhe nnọchi anya mwepụta nlele
Usoro nchọpụta
Ụgbọ njem na Nchekwa Chain oyi
Ejiri ya site na ìhè ma chekwaa na -20 ± 5 ℃;
Ndụ nchekwa
1 afọ
Ozi ịtụ
Vidiyo na-arụ ọrụ ngwa ngwa
Dee ozi gị ebe a ziga anyị ya
