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Anụ ahụ Na-eduzi PCR kit
Ngwa a na-eji sistemu nchekwa lysis pụrụ iche iji wepụta DNA ngwa ngwa site na ntanetị anụ ahụ maka mmeghachi omume PCR, yabụ ọ dabara adaba maka nnwale mkpụrụ ndụ buru ibu.
Usoro nke ịhapụ DNA genomic site na nchekwa lysis ka emechara n'ime nkeji 10-30 na 65°C. Enweghị usoro ndị ọzọ dị ka protein na iwepụ RNA, achọrọ iji DNA wepụtara dị ka ndebiri maka mmeghachi omume PCR.
2× PCR mfeNwachukwu Mix nwere nkwenye siri ike nye ndị mmeghachi omume mmeghachi omume PCR, ma nwee ike iji lysate nke ihe nlele iji nwalee dị ka ndebiri maka mmụba nke ọma na nke ọma. Ihe nyocha a nwere Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, mmeghachi omume nchekwa, PCR optimizer na stabilizer.
D-Taq DNA polymerase bụ DNA polymerase nke mepụtara nke Foregene maka mmeghachi omume PCR kpọmkwem. D-Taq DNA polymerase nwere nnagide siri ike nye ọtụtụ ndị mmeghachi omume PCR, ma nwee ike ịkọwapụta DNA dị iche iche n'ụzọ dị iche iche na usoro mmeghachi omume dị mgbagwoju anya, yana mmụbawanye ngwa ngwa nwere ike iru 2Kb / min, nke dabara adaba maka mmezi Direct PCR.
