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Ngwa Midi DNA nke ọbara (1-5ml) Ngwa Midi kewapụrụ ọbara maka DNA sitere na Ọbara
Mee ngwa ngwa genomic DNA dị elu site na ọbara anticoagulated (1-5ml).
◮Enweghị mmetọ RNase:Ogidi DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase exogenous metọọ.
◮Ọsọ ọsọ:Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;arụ ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 20-80.
◮Dị mma:A na-eme centrifugation na okpomọkụ nke ụlọ, ọ dịghịkwa mkpa maka 4 Celsius C obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA.
◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.
◮Ịdị mma Dị elu:DNA genomic nke ewepụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe nyocha dị iche iche chọrọ.
◮Sistemụ Micro-elution:Ọ nwere ike ịbawanye ntinye nke DNA genomic, nke dị mma maka nchọpụta ala ma ọ bụ nnwale.
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