Ngwa Midi DNA nke ọbara (1-5ml) Ngwa Midi kewapụrụ ọbara maka DNA sitere na Ọbara
Nkọwa ngwa:
Mee ngwa ngwa genomic DNA dị elu site na ọbara anticoagulated (1-5ml).
◮Enweghị mmetọ RNase:Ogidi DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase exogenous metọọ.
◮Ọsọ ọsọ:Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;arụ ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 20-80.
◮Dị mma:A na-eme centrifugation na okpomọkụ nke ụlọ, ọ dịghịkwa mkpa maka 4 Celsius C obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA.
◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.
◮Ịdị mma Dị elu:DNA genomic nke ewepụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe nyocha dị iche iche chọrọ.
◮Sistemụ Micro-elution:Ọ nwere ike ịbawanye ntinye nke DNA genomic, nke dị mma maka nchọpụta ala ma ọ bụ nnwale.
Nkọwa
Ihe nlele ọbara nwere ọnụ ọgụgụ buru ibu nke mkpụrụ ndụ ọbara uhie na sel platelet, nke na-eme ka nhazi ọbara na-enwe nsogbu karị.Otú ọ dị, DNA mkpụrụ ndụ ihe nketa ọbara ewepụtara site na usoro izugbe adịghị elu na ịdị ọcha yana ọdịnaya dị ntakịrị.Ngwa a na-eji Foregene Protease Plus ọhụrụ yana sistemu nchekwa BL1 na BL2 pụrụ iche, nke nwere ike gbarie ọbara mgbochi ọrịa kpamkpam nacoagulationihe nlele n'ime obere oge, si otú a na-ezere mmebi DNA ruo n'ókè kachasị ukwuu ma nweta ọnụ ọgụgụ kasị ukwuu nke DNA genomic.Usoro nhazi ọbara ngwa ngwa na ịrụ ọrụ kọlụm dị mfe na-eme ka mmịpụta nke genome ọbara dị mfe, nke mere na enwere ike nweta DNA genomic dị elu na nke dị ọcha n'ime nkeji iri anọ.
DNA-naanị silica gel akpụkpọ anụ eji na spin kọlụm nke a kit bụ ihe ọhụrụ pụrụ iche nke ụlọ ọrụ, nke ọma na kpọmkwem adsorbs DNA, na ike wepụ RNA, adịghị ọcha proteins, ion na ndị ọzọ organic ogige na cell ruo n'ókè kasị ukwuu.Iberibe DNA enwetara buru ibu, nwere ịdị ọcha dị elu, kwụsiri ike na ịdị mma a pụrụ ịdabere na ya, yana ikike iburu kọlụm ntụgharị bụ 80 μg DNA.Enwere ike iji DNA enwetara maka nnwale mkpụrụ ndụ ihe omimi dị ka mmachi mgbaze, PCR, ngwakọ ndịda, iwu ụlọ ọba akwụkwọ na ihe ndị ọzọ.Ngwa ahụ nwere ike hazie ihe ruru 5ml nke ọbara n'otu oge iji nweta oke zuru oke nke DNA genomic dị oke ọcha.
Nkọwapụta
Nkwadebe 50, nkwadebe 100, nkwadebe 250
Ngwa ngwa
| Ihe nchekwa BL1 |
| Ihe nchekwa BL2* |
| Ihe nchekwa DC |
| Ihe nchekwa PW* |
| Ihe nchekwa WB1 |
| Ihe nchekwa EB |
| Foregene Protease Plus |
| DNA-naanị kọlụm |
| Ntuziaka |
Atụmatụ & uru
-Ọnweghị mmetọ RNase: Otu kọlụm DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepu RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-ezere ụlọ nyocha ahụ ka ọ ghara imerụ ya site na RNase dịpụrụ adịpụ.
-Ọsọ ọsọ ọsọ: Foregene Protease Plus nwere ọrụ dị elu karịa proteases ndị yiri ya, ọ na-agbarikwa sample anụ ahụ ngwa ngwa;ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji iri anọ.
-dị mma: A na-eme centrifugation na ụlọ okpomọkụ, ọ dịghịkwa mkpa maka 4°C centrifugation dị ala ma ọ bụ ethanol ọdịda DNA.
-Safety: Ọ dịghị organic reagent mmịpụta a chọrọ.
-Ọdịmma dị elu: DNA genomic amịpụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe achọrọ nnwale dị iche iche.
Ngwa ngwa
Ọ dabara maka mmịpụta na sachaa DNA genomic site na ọbara ọhụrụ anticoagulated ọhụrụ ma ọ bụ oyi kpọnwụrụ.
Usoro ọrụ
Nchekwa na Shelf ndụ
Enwere ike ịchekwa ngwa a maka ọnwa 12 n'okpuru ọnọdụ akọrọ na ụlọ okpomọkụ (15-25℃);Ọ bụrụ na ọ dị mkpa ka ịchekwa ya ogologo oge, enwere ike ịchekwa ya na 2-8℃.
Rịba ama: Ọ bụrụ na echekwara ya na obere okpomọkụ, ngwọta na-adịkarị mfe mmiri ozuzo.Tupu ojiji, jide n'aka na ị ga-etinye ihe ngwọta na kit na ụlọ okpomọkụ maka oge.Ọ bụrụ na ọ dị mkpa, kpoo ya na mmiri ịsa mmiri 37 Celsius C maka nkeji 10 iji gbazee mkpọda ahụ, ma gwakọta ya tupu ejiri ya.
-Foregene Protease Plus ngwọta nwere usoro pụrụ iche, nke na-arụ ọrụ mgbe a na-echekwa ya na ụlọ okpomọkụ ruo ogologo oge (ọnwa 3), ọrụ ya na nkwụsi ike ya ga-aka mma mgbe echekwara ya na 4 Celsius C, ya mere a na-atụ aro ka ịchekwa ya na 4 Celsius C, cheta na ị gaghị edebe ya na -20.℃.
