Obere ngwa DNA
Nkọwa ngwa:
Mee ngwa ngwa DNA genomic dị elu site na ọbara anticoagulated (<1ml).
◮Enweghị mmetọ RNase:Ogidi DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase exogenous metọọ.
◮Ọsọ ọsọ:Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;arụ ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 20-80.
◮Dị mma:A na-eme centrifugation na okpomọkụ nke ụlọ, ọ dịghịkwa mkpa maka 4 Celsius C obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA.
◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.
◮Ịdị mma Dị elu:DNA genomic nke ewepụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe nyocha dị iche iche chọrọ.
◮Sistemụ Micro-elution:Ọ nwere ike ịbawanye ntinye nke DNA genomic, nke dị mma maka nchọpụta ala ma ọ bụ nnwale.
Nkọwa Kit
Ngwa a na-anabata Foregene Protease Plus ọhụrụ yana sistemu nchekwa BL1 na BL2 pụrụ iche, nke nwere ike gbarie ihe nlele ọbara na-egbu egbu kpamkpam n'ime obere oge, si otú a na-ezere mbibi DNA ruo n'ogo kachasị ma nweta oke DNA genomic.Usoro nhazi ọbara ngwa ngwa na ọrụ DNA-naanị kọlụm dị mfe na-eme ka mmịpụta nke genome ọbara dị mfe, na-eme ka DNA genomic nwee ike nweta nke dị elu na ịdị ọcha dị elu n'ime nkeji 40.
Ihe DNA-naanị silica gel membrane nke ejiri na kọlụm centrifuge nke kit a bụ ụdị ihe ọhụrụ pụrụ iche nke ụlọ ọrụ anyị, nke nwere ike jikọta DNA nke ọma na kpọmkwem, ma nwee ike wepụ RNA, adịghị ọcha, protein, ions na ihe ndị ọzọ na-emepụta ihe na sel ruo n'ókè kachasị.Iberibe DNA enwetara buru ibu, ịdị ọcha dị elu, kwụsiri ike na ịdị mma a pụrụ ịdabere na ya.Oke kacha ibu nke kọlụm DNA-naanị bụ 80 μg DNA.Enwere ike iji DNA enwetara na nyocha ihe omimi ihe omimi dị ka mgbaze enzyme, PCR, ngwakọ ndịda na iwu ụlọ ọba akwụkwọ.
Ngwa ahụ nwere ike ịhazi ihe ruru 1ml nke ọbara n'otu oge iji nweta ọnụọgụ DNA dị elu nke dị elu zuru oke.
Nkọwapụta
| Obere ngwa DNA | |||
| Ngwa ọdịnaya | DE-05111 | DE-05112 | DE-05113 |
| 50 T | 100 T | 250 T | |
| Ihe nchekwa BL1 | 15 ml | 30 ml | 75 ml |
| Ihe nchekwa BL2 * | 15 ml | 30 ml | 75 ml |
| Ihe nchekwa DC | 100 ml | 200 ml | 500 ml |
| Ihe nchekwa PW* | 25 ml | 50 ml | 125 ml |
| Ihe nchekwa WB1 | 15 ml | 30 ml | 75 ml |
| Ihe nchekwa EB | 10 ml | 20 ml | 50 ml |
| Foregene Protease Plus | 1.25 ml | 2.5 ml | 6.5 ml |
| DNA-naanị kọlụm | 50 | 100 | 250 |
| Akwụkwọ ntuziaka | 1 | 1 | 1 |
*: Buffer BL2 na Buffer PW nwere nnu mmiri mmiri na-akpasu iwe.Biko yikwasị uwe aka ma mee usoro nchebe dị mkpa mgbe ị na-arụ ọrụ.
Atụmatụ & uru
-Ọnweghị mmetọ RNase: Otu kọlụm DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepu RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-ezere ụlọ nyocha ahụ ka ọ ghara imerụ ya site na RNase dịpụrụ adịpụ.
-Ọsọ ọsọ ọsọ: Foregene Protease Plus nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji iri anọ.
-dị mma: A na-eme centrifugation na ụlọ okpomọkụ, 4 Celsius C centrifugation ma ọ bụ ethanol precipitation nke DNA adịghị achọ.
-Safety: Ọ dịghị organic reagent mmịpụta a na-eji.
-Ego dị elu: Iberibe DNA genomic amịpụtara buru ibu, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe achọrọ nnwale dị iche iche.
Ngwa ngwa
Ọ dabara maka mmịpụta na sachaa DNA genomic site na ọbara ọhụrụ anticoagulated ọhụrụ ma ọ bụ oyi kpọnwụrụ.
Usoro ọrụ
Nchekwa na ndụ nchekwa
Enwere ike ịchekwa ngwa a maka ọnwa 12 n'okpuru ọnọdụ akọrọ na ụlọ okpomọkụ (15-25 ° C);Ọ bụrụ na achọrọ ịchekwa ya ogologo oge, enwere ike ịchekwa ya na 2-8 Celsius.
Rịba ama: Ọ bụrụ na echekwara ya na obere okpomọkụ, ngwọta na-adịkarị mfe mmiri ozuzo.Tupu ojiji, jide n'aka na ị ga-etinye ihe ngwọta na kit na ụlọ okpomọkụ maka oge.Ọ bụrụ na ọ dị mkpa, kpoo ya na mmiri ịsa mmiri 37 Celsius C maka nkeji 10 iji gbazee mkpọda ahụ, ma gwakọta ya tupu ejiri ya.
Ihe ngwọta Foregene Protease Plus nwere usoro pụrụ iche, nke na-arụ ọrụ mgbe a na-echekwa ya na ụlọ okpomọkụ ruo ogologo oge (ọnwa 3);ọ ga-adịkwu ike ma kwụsie ike mgbe echekwara ya na 4 Celsius C, ya mere, a na-atụ aro ka ịchekwa ya na 4 Celsius C, cheta ka ọ ghara idobe ya na -20 Celsius.
