Blood SuperDirectᵀᴹ PCR Kit-EDTA Blood Direct PCR Master Mix for Genotyping of Blood
Nkọwa ngwa:
Jiri EDTA zuru oke anticoagulated ozugbo dị ka ndebiri maka nkwalite PCR, yana mmetụta dị elu yana arụmọrụ nkwalite dị elu.
◮Ọ dịghị mkpa maka ịdị ọcha DNA na-ewe oge ma dị oke ọnụ, enweghị usoro ọgwụgwọ, na iji ọbara dum mee ihe ozugbo dị ka ndebiri PCR.
◮Usoro ahụ nwere ikike mmụba siri ike ma nwee ike ịchọpụta genome na iberibe DNA lekwasịrị anya nke mba ọzọ n'ime ọbara.
◮2× Dị mfeTMMix-EDTA Nnukwu nnabata ọbara: ruo 45% n'ọbara mmadụ yana 20% n'ọbara òké.
◮A na-emechi ihe nlele ahụ nke ọma, yabụ na ọ dịghị mkpa ichegbu onwe gị maka mmetọ nlele na nsonaazụ PCR adịgboroja.
Nkọwapụta
200 × 20μl rxns, 2000 × 20μl rxns
2× Dị mfeTMMix-EDTA: Nwere D-Taq DNA Polymerase emezigharịrị pụrụ iche, dNTPs, MgCl2, ihe nchekwa mmeghachi omume, nkwalite mmeghachi omume PCR, optimizer na stabilizer, wdg. Na mmeghachi omume PCR, tinye naanị ọbara zuru oke anticoagulated kwesịrị ekwesị, primers, na ddH.2O na 2× SuperEasy kwekọrọTMMix-EDTA ga-eji na nzaghachi PCR.
6× DNA Loading Buffer: Ebe nchekwa ihe enweghị SDS.A na-atụ aro ka iji 6 × DNA Loading Buffer gụnyere na kit mgbe ị na-eme agarose gel electrophoresis iji nweta nsonaazụ electrophoresis dị mma.Ejila Loading Buffer nwere SDS, ma ọ bụghị ya, a ga-enwe nnukwu ọdụ ọkụ na-egbuke egbuke n'okporo ụzọ n'oge electrophoresis, nke ga-emetụta nsonaazụ nnwale.
Ngwa ngwa
| 2× Dị mfeTMNgwakọta-EDTA |
| Ihe nchekwa 6 × DNA na-ebufe |
| Ntuziaka |
Atụmatụ & uru
-Ọ dịghị mkpa maka ịdị ọcha DNA na-ewe oge ma dị oke ọnụ, ọ dịghị pretreatment, na iji ọbara dum mee ihe kpọmkwem dị ka ihe atụ PCR.
- Usoro ahụ nwere ikike mmụba siri ike ma nwee ike ịchọpụta genome na iberibe DNA lekwasịrị anya nke mba ọzọ n'ime ọbara.
-2× SuperEasyTMMix-EDTA Nnukwu nnabata ọbara: ruo 45% n'ọbara mmadụ yana 20% n'ọbara òké.
- A na-emechi ihe nlele ahụ n'ụzọ zuru ezu, yabụ na ọ dịghị mkpa ichegbu onwe gị maka mmetọ nlele na nsonaazụ PCR adịgboroja.
Ngwa ngwa
A raara onwe ya nye iji duzie njirimara PCR nke EDTA na-egbochi ọbara ọgbụgba.(Gụnyere: ọbara mmadụ, ọbara òké, ọbara ọkụkọ, ọbara nnụnụ, ọbara ehi, ọbara nkịta, wdg)
Usoro ọrụ
Nchekwa na Shelf ndụ
Ekwesịrị ịchekwa ngwa a na -20 Celsius.
2× Dị mfeTMMix-EDTA, ọ bụrụ na-eji ya ugboro ugboro, ọ nwekwara ike ịchekwa na 4 ℃ maka obere oge nchekwa (jiri elu n'ime 10 ụbọchị).
6 × DNA Loading Buffer, enwere ike ịchekwa ya na 4°C ma ọ bụ -20°C ogologo oge.
