Ngwa Mwepụta Gel Ngwa DNA Gel Extraction Kit
Nkọwa ngwa:
Ngwa ngwa na nke ọma nwetaghachi iberibe DNA 20bp-10kb sitere na gel agarose.
◮Ogologo mgbake DNA dị iche iche:Enwere ike nwetaghachi iberibe DNA dị mkpụmkpụ dịka 30bp yana buru ibu dịka 10kb.
◮Ịrụ mgbake dị elu:Ọrụ mgbake kachasị elu nwere ike iru karịa 80%.
◮Ngosipụta nke obere sistemu:ọ dịkarịa ala 30μl elution ngwọta nwere ike iji maka elution, nke nwere ike ime ka ntinye nke iberibe DNA nwetaghachi nke ọma.
◮Ọsọ ọsọ:Ọ dị mfe ịrụ ọrụ, mgbake DNA nwere ike mechaa n'ime nkeji iri na ise.
◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.
◮Ịdị mma Dị elu:Iberibe DNA ndị ahụ enwetara dị oke ọcha, nke nwere ike izute nnwale dị iche iche na-esote.
Nkọwa
Ngwa a na-eji kọlụm na usoro nke ụlọ ọrụ anyị mepụtara, nke nwere ike nwetaghachi iberibe DNA dị ọcha nke ọma site na gel agarose.Ngwa ahụ nwere ike nwetaghachi iberibe DNA n'ọtụtụ buru ibu.N'okpuru ọnọdụ nkịtị, enwere ike nwetaghachi iberibe DNA nke 30bp-10kb.Enwere ike iji opekempe 30μl nke eluent mee ka mkpokọta DNA nwetagoro dị elu.
Ngwa ahụ dị mfe iji rụọ ọrụ ma nwee usoro ole na ole.Ọ chọrọ naanị centrifugation dị iche iche iji hazie ọtụtụ ihe nlele n'otu oge, enwere ike nweta iberibe DNA enwetara dị ọcha na nkeji iri na ise.
Nkọwapụta
50 nkwadebe
Buffer GE: Gbanyụọ ngọngọ gel na 60 Celsius ka ịhapụ iberibe DNA na gel.
Buffer WB1: Wepu ion nnu fọdụrụ na DNA.
Buffer EB: wepụta DNA na akpụkpọ ahụ kọlụm dị ọcha.
Kọlụm DNA-naanị: Mgbasa ozi a kapịrị ọnụ nke iberibe DNA na gel.
Ngwa ngwa
| Ihe nchekwa GE* |
| Ihe nchekwa WB1 |
| Ihe nchekwa EB |
| DNA-naanị kọlụm |
| Ntuziaka |
Atụmatụ & uru
- Ogologo mgbake DNA zuru oke: enwere ike nwetaghachi iberibe DNA dị mkpụmkpụ dịka 30bp yana buru ibu dịka 10kb.
-Ike mgbake dị elu: arụmọrụ mgbake kachasị elu nwere ike iru karịa 80%.
-Eletion nke obere usoro: ọ dịkarịa ala 30μl ngwọta elution nwere ike iji maka elution, nke nwere ike ịbawanye ntinye nke iberibe DNA enwetara nke ọma.
-Ọsọ ọsọ ọsọ: Ọ dị mfe iji rụọ ọrụ, enwere ike mezue mgbake DNA n'ime nkeji iri na ise.
-Safety: Ọ dịghị organic reagent mmịpụta a chọrọ.
-Ego dị elu: Iberibe DNA ndị ahụ enwetara dị oke ọcha, nke nwere ike izute nnwale dị iche iche na-esote.
Ngwa ngwa
Ngwa a dabara adaba maka iweghachi iberibe DNA (30bp-10kb) na gels agarose.
Usoro ọrụ
Nchekwa na Shelf ndụ
Enwere ike ịchekwa ngwa a maka ọnwa 12 n'okpuru ọnọdụ akọrọ na ụlọ okpomọkụ (15-25 ° C), ọ bụrụ na ọ dị mkpa ka ịchekwa ya ogologo oge, enwere ike ịchekwa ya na 2-8 ° C.
Rịba ama: Ọ bụrụ na echekwara ya na obere okpomọkụ, ngwọta na-adịkarị mfe mmiri ozuzo.Tupu ojiji, jide n'aka na ị ga-etinye ihe ngwọta na kit na ụlọ okpomọkụ maka oge.Ọ bụrụ na ọ dị mkpa, kpoo ya na mmiri ịsa mmiri 37 Celsius C maka nkeji 10 iji gbazee mkpọda ahụ, ma gwakọta ya tupu ejiri ya.
