Blood SuperDirectᵀᴹ PCR Kit (UNG) -EDTA Blood Direct PCR Master Mix for Genotyping of Blood
Nkọwa ngwa:
Jiri EDTA zuru oke anticoagulated ozugbo dị ka ndebiri maka nkwalite PCR, yana mmetụta dị elu yana arụmọrụ nkwalite dị elu.Agbakwunyela usoro mgbochi mmetọ nke UNG iji gbochie ihe dị mma.
◮Ọ dịghị mkpa maka ịdị ọcha DNA na-ewe oge ma dị oke ọnụ, enweghị usoro ọgwụgwọ, na iji ọbara dum mee ihe ozugbo dị ka ndebiri PCR.
◮Usoro ahụ nwere ikike mmụba siri ike ma nwee ike ịchọpụta genome na iberibe DNA lekwasịrị anya nke mba ọzọ n'ime ọbara.
◮2× Dị mfeTMMix (UNG) -EDTA nwere oke nnabata ọbara: ruo 45% n'ọbara mmadụ yana 20% n'ọbara òké.
◮A na-emechi ihe nlele ahụ nke ọma, yabụ na ọ dịghị mkpa ichegbu onwe gị maka mmetọ nlele na nsonaazụ PCR adịgboroja.Sistemụ PCR na-egbochi mmetọ
◮2×SuperEasyTMMix (UNG) -EDTA, na-ewepụ mmetọ nke ngwaahịa PCR nke ọma, wee hụ na nkọwapụta na izi ezi nke nkwalite.
Nkọwapụta
200 × 20μl rxns, 2000 × 20μl rxns
2× Dị mfeTMNgwakọta (UNG) -EDTA: Na ndabere nke 2 × SuperEasyTMMix-EDTA, dUTP na-eji kama dTTP, na UNG enzyme nke nwere ike imebi template nwere dUTP na-agbakwunyere n'otu oge ahụ, nke nwere ike igbochi nke ọma mmetọ nke PCR amplification ngwaahịa.Nwere D-Taq DNA Polymerase emezigharịrị pụrụ iche, dNTPs, MgCl2, ihe nchekwa mmeghachi omume, ihe nkwalite mmeghachi omume PCR, optimizer na stabilizer, wdg. Na mmeghachi omume PCR, tinye naanị ọbara zuru oke anticoagulated kwesịrị ekwesị, primers, na ddH.2O na 2× SuperEasy kwekọrọTMNgwakọta (UNG) -EDTA ga-eji na mmeghachi omume PCR.
6 × DNA Loading Buffer: Ihe nkpuchi anaghị enwe SDS.A na-atụ aro ka iji 6 × DNA Loading Buffer gụnyere na kit mgbe ị na-eme agarose gel electrophoresis iji nweta nsonaazụ electrophoresis dị mma.Ejila Loading Buffer nwere SDS, ma ọ bụghị ya, a ga-enwe nnukwu ọdụ ọkụ na-egbuke egbuke n'okporo ụzọ n'oge electrophoresis, nke ga-emetụta nsonaazụ nnwale.
Ngwa ngwa
| 2× Dị mfeTMNgwakọta (UNG) -EDTA |
| Ihe nchekwa 6 × DNA na-ebufe |
| Ntuziaka |
Atụmatụ & uru
- Ọ dịghị mkpa maka ịdị ọcha DNA na-ewe oge ma dị oke ọnụ, enweghị ọgwụgwọ ọ bụla, na iji ọbara dum mee ihe kpọmkwem dị ka ụdị PCR.
- Usoro ahụ nwere ikike mmụba siri ike ma nwee ike ịchọpụta genome na iberibe DNA lekwasịrị anya nke mba ọzọ n'ime ọbara.
-2× SuperEasyTMMix (UNG) -EDTA nwere oke nnabata ọbara: ruo 45% n'ọbara mmadụ yana 20% n'ọbara òké.
- A na-emechi ihe nlele ahụ n'ụzọ zuru ezu, yabụ na ọ dịghị mkpa ichegbu onwe gị maka mmetọ nlele na nsonaazụ PCR adịgboroja.Sistemụ PCR na-emegide mmetọ 2 × SuperEasyTMMix (UNG) -EDTA, na-ewepụ mmetọ nke ngwaahịa PCR nke ọma, wee hụ na nkọwapụta na izi ezi nke nkwalite.
Ngwa ngwa
A raara onwe ya nye iji duzie njirimara PCR nke EDTA na-egbochi ọbara ọgbụgba.(Gụnyere: ọbara mmadụ, ọbara òké, ọbara ọkụkọ, ọbara nnụnụ, ọbara ehi, ọbara nkịta, wdg)
Usoro ọrụ
Nchekwa na Shelf ndụ
A na-echekwa ngwa a na -20 Celsius.
2× Dị mfeTMMix(UNG) -EDTA ， Ọ bụrụ na ejiri ya ugboro ugboro, enwere ike ịchekwa ya na 4 Celsius maka nchekwa obere oge (jiri n'ime ụbọchị iri).
6 × DNA Loading Buffer, Enwere ike ịchekwa ya na 4°C ma ọ bụ -20°C ogologo oge.
