Blood SuperDirectᵀᴹ PCR Kit (UNG) -Heparin Blood Direct PCR Master Mix for Genotyping of Blood
Nkọwa ngwa:
Jiri heparin anticoagulated ọbara niile ozugbo dị ka ndebiri maka nkwalite PCR, yana mmetụta dị elu yana arụmọrụ nkwalite dị elu.Agbakwunyela usoro mgbochi mmetọ nke UNG iji gbochie ihe dị mma.
◮Usoro ahụ nwere ikike mmụba siri ike ma nwee ike ịchọpụta genome na iberibe DNA lekwasịrị anya nke mba ọzọ n'ime ọbara.
◮2× Dị mfeTMMix (UNG) - Heparin High ọbara ndidi: ruo 45% n'ime ọbara mmadụ na 20% na ọbara òké.
◮A na-emechi ihe nlele ahụ nke ọma, yabụ na ọ dịghị mkpa ichegbu onwe gị maka mmetọ nlele na nsonaazụ PCR adịgboroja.Sistemụ PCR na-egbochi mmetọ
◮2×SuperEasyTMNgwakọta (UNG), kpochapụ mmetọ nke ngwaahịa PCR na-ebute nke ọma, wee hụ na nkọwapụta na izi ezi nke nkwalite.
Nkọwapụta
200 × 20μl rxns, 2000 × 20μl rxns
2× Dị mfeTMMix (UNG) -Heparin: Na ndabere nke 2 × SuperEasyTMMix-Heparin, dUTP na-eji kama dTTP, na UNG enzyme nke nwere ike imebi ihe dUTP template na-agbakwunyere n'otu oge ahụ, nke nwere ike igbochi mmetọ nke PCR amplification ngwaahịa.Nwere D-Taq DNA Polymerase emezigharịrị pụrụ iche, dNTPs, MgCl2, ihe nchekwa mmeghachi omume, ihe nkwalite mmeghachi omume PCR, optimizer na stabilizer, wdg. Na mmeghachi omume PCR, tinye naanị ọbara zuru oke anticoagulated kwesịrị ekwesị, primers, na ddH.2O na 2 × SuperEasyTM Mix (UNG) -Heparin dabara adaba na mmeghachi omume PCR.
6× DNA Loading Buffer: Ebe nchekwa ihe enweghị SDS.A na-atụ aro ka iji 6 × DNA Loading Buffer gụnyere na kit mgbe ị na-eme agarose gel electrophoresis iji nweta nsonaazụ electrophoresis dị mma.Ejila Loading Buffer nwere SDS, ma ọ bụghị ya, a ga-enwe nnukwu ọdụ ọkụ na-egbuke egbuke n'okporo ụzọ n'oge electrophoresis, nke ga-emetụta nsonaazụ nnwale.
Ngwa ngwa
| 2× Dị mfeTMNgwakọta (UNG) - Heparin |
| Ihe nchekwa 6 × DNA na-ebufe |
| Ntuziaka |
Atụmatụ & uru
-Usoro ahụ nwere ikike mmụba siri ike ma nwee ike ịchọpụta genome na iberibe DNA lekwasịrị anya nke mba ọzọ n'ime ọbara.
-2× SuperEasyTMMix (UNG) - Heparin High ọbara ndidi: ruo 45% n'ime ọbara mmadụ na 20% na ọbara òké.
- A na-emechi ihe nlele ahụ n'ụzọ zuru ezu, yabụ na ọ dịghị mkpa ichegbu onwe gị maka mmetọ nlele na nsonaazụ PCR adịgboroja.Sistemụ PCR na-emegide mmetọ 2 × SuperEasyTMNgwakọta (UNG), kpochapụ mmetọ nke ngwaahịa PCR na-ebute nke ọma, wee hụ na nkọwapụta na izi ezi nke nkwalite.
Ngwa ngwa
A raara onwe ya nye maka iduzi PCR njirimara nke Heparin anticoagulated ọbara dum.(Gụnyere: ọbara mmadụ, ọbara òké, ọbara ọkụkọ, ọbara nnụnụ, ọbara ehi, ọbara nkịta, wdg)
Usoro ọrụ
Nchekwa na Shelf ndụ
Usoro dum nke ụgbọ njem igbe ice dị ala, iji hụ na ngwa ahụ dị na ọnọdụ <4 ℃.
2× Dị mfeTMMix (UNG) -Heparin, ọ bụrụ na ejiri ya ugboro ugboro, enwere ike ịchekwa ya na 4 Celsius maka nchekwa oge dị mkpirikpi (jiri n'ime ụbọchị 10).
6 × DNA Loading Buffer, enwere ike ịchekwa ya na 4°C ma ọ bụ -20°C ogologo oge.
