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Cell Direct RT qPCR Kit
Ngwa a na-eji sistemụ lysis pụrụ iche nke nwere ike ịhapụ RNA ngwa ngwa site na mkpụrụ ndụ sel ka etolite maka mmeghachi omume RT-qPCR, wee si otú ahụ wepụ usoro ịdị ọcha RNA na-eri oge ma na-arụsi ọrụ ike. Enwere ike ịnweta template RNA naanị na nkeji 7. Ihe ngwanrọ 5 × Direct RT Mix na 2 × Direct qPCR Mix-SYBR reagents nyere site na kit nwere ike ị nweta ngwa ngwa PCR ọnụọgụ ezigbo oge.
5, Direct RT Mix na 2 × Direct qPCR Mix-SYBR nwere nnwere onwe mmechi siri ike, na lysate nke ihe atụ ahụ nwere ike iji dị ka ndebiri maka RT-qPCR ozugbo. Ngwa a nwere ihe pụrụ iche RNA dị elu Foregene na-agbanwe transcriptase, na Hot D-Taq DNA polymerase, dNTPs, MgCl2, ihe mmeghachi omume mmeghachi omume, PCR optimizer na stabilizer.
