Ngwa DNA kewapụ mmiri DNA mmịpụta na ihe dị ọcha maka mmiri
Nkọwapụta
50 nkwadebe
- Lysozyme: enzymatically hydrolyze cell mgbidi nke nti bacteria.
-Buffer TE: eji akwadebe 100mg / ml Lysozyme ngwọta ma nye lysozyme enzymatic hydrolysis gburugburu ebe obibi.
-Buffer SG1 & Buffer SG2: Nye ihe nlele gburugburu mgbaze protease.
-Foregene Protease: Enzymatically hydrolyze samples na protease enzymatic gburugburu iji hapụ genomic DNA.
-Buffer SG3: Inactivate Foregene Protease na-enye ebe ntinye DNA.
-Buffer SG4: Mgbakwunye na-enye gburugburu DNA loading.
-Buffer PW: Wepu adịghị ọcha dị ka protein na RNA na DNA.
-Buffer WB: Wepu ion nnu fọdụrụ na DNA.
-Buffer EB: elute DNA na akpụkpọ ahụ kọlụm dị ọcha.
-DNA-na kọlụm: kpọmkwem adsorb DNA genomic na lysate.
Ngwa ngwa
Ihe nchekwa SG1 |
Ihe nchekwa SG2 |
Ihe nchekwa SG3 |
Ihe nchekwa SG4 |
Ihe nchekwa PW |
Ihe nchekwa WB |
Ihe nchekwa EB |
Buffer TE |
Protease nke mbụ |
Lysozyme |
DNA-naanị kọlụm |
Ntuziaka |
Atụmatụ & uru
-Ọnweghị mmetọ RNase: Otu kọlụm DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepu RNA na DNA genomic na-enweghị RNase agbakwunyere n'oge nnwale ahụ, na-ezere ụlọ nyocha ahụ ka emetọọ ya site na RNase dịpụrụ adịpụ.
-Ọsọ ọsọ ọsọ: Ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 40.
-Ọ dị mma: A na-eme centrifugation na ụlọ okpomọkụ, ọ dịghịkwa mkpa maka 4 Celsius C dị ala ma ọ bụ ethanol precipitation nke DNA.
-Safety: ọ dịghị mkpa organic reagent mmịpụta.
-Ego dị elu: Iberibe DNA genomic amịpụtara buru ibu, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe achọrọ nnwale dị iche iche.
Ngwa ngwa
Ọ dabara maka ịdị ọcha nke DNA genomic nke ihe atụ ndị a: mmiri ọdọ mmiri aquaculture, mmiri ọdọ mmiri, mmiri mgbata, mmiri ọdọ mmiri, mmiri osimiri, mmiri ọdọ mmiri lotus na ihe atụ ndị ọzọ.
Usoro ọrụ
Nchekwa na Shelf ndụ
-Enwere ike ịchekwa ngwa a maka ọnwa 12 n'okpuru ọnọdụ akọrọ na ụlọ okpomọkụ (15-25 ° C), ọ bụrụ na ọ dị mkpa ka echekwara ya ogologo oge, enwere ike ịchekwa ya na 2-8 ° C.
Rịba ama: Ọ bụrụ na echekwara ya na obere okpomọkụ, ngwọta na-adịkarị mfe mmiri ozuzo.Tupu iji ya, jide n'aka na ị ga-etinye ngwọta na kit na ụlọ okpomọkụ ruo oge ụfọdụ.Ọ bụrụ na ọ dị mkpa, kpochaa ya na nkeji 37°C mmiri ịsa ahụ maka nkeji 10 iji gbazee mmiri ozuzo, ma gwakọta ya tupu ejiri ya.
-Foregene Protease ngwọta nwere usoro pụrụ iche, nke na-arụ ọrụ mgbe echekwara na ụlọ okpomọkụ ruo ogologo oge (ọnwa 3);mgbe echekwara na 4 ℃, ọrụ ya na nkwụsi ike ga-aka mma, ya mere a na-atụ aro ka ịchekwa ya na 4 ℃, cheta na ị gaghị echekwa ya na -20 ℃.
-A na-echekwa ntụ ntụ akọrọ Lysozyme na -20 Celsius;A na-ekewa ngwọta Lysozyme a kwadebere n'ime obere akụkụ ma chekwaa ya na -20 Celsius.