-
Anụ ahụ Na-eduzi PCR kit
Ngwa a na-eji sistemu nchekwa lysis pụrụ iche iji wepụta DNA ngwa ngwa site na ntanetị anụ ahụ maka mmeghachi omume PCR, yabụ ọ dabara adaba maka nnwale mkpụrụ ndụ buru ibu.
Usoro nke ịhapụ DNA genomic site na nchekwa lysis ka emechara n'ime nkeji 10-30 na 65°C. Enweghị usoro ndị ọzọ dị ka protein na iwepụ RNA, achọrọ iji DNA wepụtara dị ka ndebiri maka mmeghachi omume PCR.
2× PCR mfeNwachukwu Mix nwere nkwenye siri ike nye ndị mmeghachi omume mmeghachi omume PCR, ma nwee ike iji lysate nke ihe nlele iji nwalee dị ka ndebiri maka mmụba nke ọma na nke ọma. Ihe nyocha a nwere Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, mmeghachi omume nchekwa, PCR optimizer na stabilizer.
D-Taq DNA polymerase bụ DNA polymerase nke mepụtara nke Foregene maka mmeghachi omume PCR kpọmkwem. D-Taq DNA polymerase nwere nnagide siri ike nye ọtụtụ ndị mmeghachi omume PCR, ma nwee ike ịkọwapụta DNA dị iche iche n'ụzọ dị iche iche na usoro mmeghachi omume dị mgbagwoju anya, yana mmụbawanye ngwa ngwa nwere ike iru 2Kb / min, nke dabara adaba maka mmezi Direct PCR.
-
Ké ọdụ SuperDirect PCR Kit
Ngwa a na - eji sistemu nchekwa lysis pụrụ iche iji wepụta DNA ngwa ngwa site na ọdụ oke na ntị ntị maka PCR mmeghachi omume, yabụ ọ dabara adaba maka nnwale mkpụrụ ndụ buru ibu.
Usoro nke ịhapụ DNA genomic site na nchekwa lysis agwụla n'ime 10-30 nkeji na ụlọ okpomọkụ (20-25 ° C). Enweghị kpo oku achọrọ, usoro ndị ọzọ dịka protein na iwepụ RNA adịghị mkpa. Enwere ike iji DNA enwere ike wepụtara dị ka Template maka mmeghachi PCR.
2, M-PCR Easy TM Mix nwere nnagide siri ike nye ndị mmechi mmeghachi omume PCR, ma nwee ike iji lysate nke nlele ahụ nwalee ya dị ka ndebiri maka mmụba nke ọma na nke ọma. Ihe nyocha a nwere Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, nchekwa nchekwa, PCR optimizer na stabilizer.
D-Taq DNA polymerase bụ DNA polymerase nke mepụtara nke Foregene maka mmeghachi omume PCR kpọmkwem. D-Taq DNA polymerase nwere nnagide siri ike nye ọtụtụ ndị mmeghachi omume PCR, ma nwee ike ịkọwapụta DNA dị iche iche n'ụzọ dị iche iche na usoro mmeghachi omume dị mgbagwoju anya, yana mmụbawanye ngwa ngwa nwere ike iru 2Kb / min, nke dabara adaba maka mmezi Direct PCR.
-
Anụ Ahụ Na-eduzi PCR kit-UNG
Ngwa a na-eji sistemu nchekwa lysis pụrụ iche iji wepụta DNA ngwa ngwa site na ntanetị anụ ahụ maka mmeghachi omume PCR, yabụ ọ dabara adaba maka nnwale mkpụrụ ndụ buru ibu.
Usoro nke ịhapụ DNA genomic site na nchekwa lysis ka emechara n'ime nkeji 10-30 na 65°C. Enweghị usoro ndị ọzọ dị ka protein na mwepụ RNA achọrọ, a ga-ejikwa DNA nke wepụtara dị ka ndebiri maka mmeghachi omume PCR.
2×PCR mfeNwachukwu Mix (UNG) jiri dUTP kama dTTP na ndabere nke 2×PCR mfeNwachukwu Gwakọta, ma gbakwunye enzyme UNG (Uracil-N-glycosylase) nke nwere ike iweda ndebiri nwere dUTP n'otu oge. Tupu mmeghachi omume PCR, a na-eji enzyme UNG mebie ngwaahịa PCR nwere uracil. UNG enzyme agaghị enwe mmetụta ọ bụla na ndebiri nke na-enweghị uracil, si otú ahụ na-achọpụta kpọmkwem na njupụta nke mmụba ma na-egbochi ohere nke PCR ngwaahịa mmetọ n'oge nnukwu mkpụrụ ndụ ihe nketa.
D-Taq DNA polymerase bụ DNA polymerase nke mepụtara nke Foregene maka mmeghachi omume PCR kpọmkwem. D-Taq DNA polymerase nwere nnagide siri ike nye ọtụtụ ndị mmeghachi omume PCR, ma nwee ike ịkọwapụta DNA dị iche iche n'ụzọ dị iche iche na usoro mmeghachi omume dị mgbagwoju anya, yana mmụbawanye ngwa ngwa nwere ike iru 2Kb / min, nke dabara adaba maka mmezi Direct PCR.
-
Cell Direct RT qPCR Kit
Ngwa a na-eji sistemụ lysis pụrụ iche nke nwere ike ịhapụ RNA ngwa ngwa site na mkpụrụ ndụ sel ka etolite maka mmeghachi omume RT-qPCR, wee si otú ahụ wepụ usoro ịdị ọcha RNA na-eri oge ma na-arụsi ọrụ ike. Enwere ike ịnweta template RNA naanị na nkeji 7. Ihe ngwanrọ 5 × Direct RT Mix na 2 × Direct qPCR Mix-SYBR reagents nyere site na kit nwere ike ị nweta ngwa ngwa PCR ọnụọgụ ezigbo oge.
5, Direct RT Mix na 2 × Direct qPCR Mix-SYBR nwere nnwere onwe mmechi siri ike, na lysate nke ihe atụ ahụ nwere ike iji dị ka ndebiri maka RT-qPCR ozugbo. Ngwa a nwere ihe pụrụ iche RNA dị elu Foregene na-agbanwe transcriptase, na Hot D-Taq DNA polymerase, dNTPs, MgCl2, ihe mmeghachi omume mmeghachi omume, PCR optimizer na stabilizer.
-
Ké ọdụ Direct PCR Kit
Ngwa a na-eji sistemụ lysis pụrụ iche iji wepụta DNA genomic ngwa ngwa na ọdụ, ntị, mọzụlụ na anụ ahụ ndị ọzọ maka mmeghachi omume PCR, yabụ ọ dabara adaba maka nnwale mkpụrụ ndụ buru ibu.
Usoro nke ịhapụ DNA genomic site na nchekwa lysis ka emechara n'ime 10-30 nkeji na 65 Celsius. Enweghị usoro ndị ọzọ dị ka protein na mwepụ RNA achọrọ, na DNA nwere ike iji dị ka ndebiri maka mmeghachi omume PCR.
2 × M1-PCR Easy TM Mix nwere nnagide siri ike nye ndị mmechi mmeghachi omume PCR, ma nwee ike iji lysate nke nlele ahụ nwalee ya dị ka ndebiri maka mmụba nke ọma na nke a kapịrị ọnụ. Ihe nyocha a nwere Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, nchekwa nchekwa, PCR optimizer na stabilizer.
D-Taq DNA polymerase bụ DNA polymerase nke mepụtara nke Foregene maka mmeghachi omume PCR kpọmkwem. D-Taq DNA polymerase nwere nnagide siri ike nye ọtụtụ ndị mmeghachi omume PCR, ma nwee ike ịkọwapụta DNA dị iche iche n'ụzọ dị iche iche na usoro mmeghachi omume dị mgbagwoju anya, yana mmụbawanye ngwa ngwa nwere ike iru 2Kb / min, nke dabara adaba maka mmezi Direct PCR.
-
Ké Ta Direct PCR Kit-UNG
Ngwa a na-eji sistemụ lysis pụrụ iche iji wepụta DNA genomic ngwa ngwa na ọdụ, ntị, mọzụlụ na anụ ahụ ndị ọzọ maka mmeghachi omume PCR, yabụ ọ dabara adaba maka nnwale mkpụrụ ndụ buru ibu.
Usoro nke ịhapụ DNA genomic site na nchekwa lysis ka emechara n'ime 10-30 nkeji na 65 Celsius. Enweghị usoro ndị ọzọ dị ka protein na mwepụ RNA achọrọ, na DNA nwere ike iji dị ka ndebiri maka mmeghachi omume PCR.
2 × M1-PCR Easy TM Mix (UNG) na-eji dUTP kama dTTP na ndabere nke 2 × M1-PCR Easy TM Mix (UNG), ma gbakwunye enzyme UNG (Uracil-N-glycosylase) nke nwere ike iweda ndebiri nke nwere dUTP na otu oge. Tupu mmeghachi omume PCR, a na-eji enzyme UNG mebie ngwaahịa PCR nwere uracil. UNG enzyme agaghị enwe mmetụta ọ bụla na ndebiri nke na-enweghị uracil, si otú a na-ahụ oke nkọwa na izi ezi nke mmụba ma gbochie ohere nke nnwale mkpụrụ ndụ buru ibu. Mgbawa nke ngwaahịa PCR mere.
D-Taq DNA polymerase bụ DNA polymerase nke mepụtara nke Foregene maka mmeghachi omume PCR kpọmkwem. D-Taq DNA polymerase nwere nnagide siri ike nye ọtụtụ ndị mmeghachi omume PCR, ma nwee ike ịkọwapụta DNA dị iche iche n'ụzọ dị iche iche na usoro mmeghachi omume dị mgbagwoju anya, yana mmụbawanye ngwa ngwa nwere ike iru 2Kb / min, nke dabara adaba maka mmezi Direct PCR.
-
Ahịhịa Zebra Kwụsị Kit PCR
Ngwa a na-eji sistemu nchekwa lysis pụrụ iche iji wepụta DNA genomic ngwa ngwa site na zebrafish na anụ ndị ọzọ dị na azụ, nku ọdụ ma ọ bụ akwa akwa azụ maka mmeghachi omume PCR, yabụ ọ dabara adaba maka nnwale mkpụrụ ndụ buru ibu. Usoro nke ịhapụ DNA genomic site na nchekwa lysis ka emechara n'ime 10-30 nkeji na 65 Celsius. Enweghị usoro ndị ọzọ dị ka protein na mwepụ RNA achọrọ, na DNA nwere ike iji dị ka ndebiri maka mmeghachi omume PCR.
2, PCR Easy TM Mix nwere nnagide siri ike nye ndị mmeghachi omume PCR, ma nwee ike iji lysate nke nlele ahụ nwalee ya dị ka ndebiri maka mmụba nke ọma na nke ọma. Ihe nyocha a nwere ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, nchekwa nchekwa, PCR optimizer na stabilizer. Ejiri ya na nchekwa lysis nwere ike ịchọpụta ngwa ngwa na ngwa ngwa ọ nwere njirimara nke nwere nnukwu uche, nkọwa siri ike, na ezi nkwụsi ike.
D-Taq DNA polymerase bụ DNA polymerase nke mepụtara nke Foregene maka mmeghachi omume PCR kpọmkwem. D-Taq DNA polymerase nwere nnagide siri ike nye ọtụtụ ndị mmeghachi omume PCR, ma nwee ike ịkọwapụta DNA dị iche iche n'ụzọ dị iche iche na usoro mmeghachi omume dị mgbagwoju anya, yana mmụbawanye ngwa ngwa nwere ike iru 2Kb / min, nke dabara adaba maka mmezi Direct PCR.
-
Azụ Zebra Na-eduzi PCR Kit-UNG
Ngwa a na-eji sistemu nchekwa lysis pụrụ iche iji wepụta DNA genomic ngwa ngwa site na zebrafish na anụ ndị ọzọ dị na azụ, nku ọdụ ma ọ bụ akwa akwa azụ maka mmeghachi omume PCR, yabụ ọ dabara adaba maka nnwale mkpụrụ ndụ buru ibu. Usoro nke ịhapụ DNA genomic site na nchekwa lysis ka emechara n'ime 10-30 nkeji na 65 Celsius. Enweghị usoro ndị ọzọ dị ka protein na mwepụ RNA achọrọ, na DNA nwere ike iji dị ka ndebiri maka mmeghachi omume PCR.
2, PCR Easy TM Mix (UNG) na-eji dUTP kama dTTP na ndabere nke 2 × PCR Easy TM Mix, ma na-agbakwunye UNG enzyme (Uracil-N-glycosylase) nke nwere ike iweda ndebiri nwere dUTP n'otu oge. Tupu mmeghachi omume PCR, a na-eji enzyme UNG mebie ngwaahịa PCR nwere uracil. UNG enzyme agaghị enwe mmetụta ọ bụla na ndebiri nke na-enweghị uracil, si otú a na-ahụ oke nkọwa na izi ezi nke mmụba ma gbochie ohere nke nnwale mkpụrụ ndụ buru ibu. Mgbawa nke ngwaahịa PCR mere.
D-Taq DNA polymerase bụ DNA polymerase nke mepụtara nke Foregene maka mmeghachi omume PCR kpọmkwem. D-Taq DNA polymerase nwere nnagide siri ike nye ọtụtụ ndị mmeghachi omume PCR, ma nwee ike ịkọwapụta DNA dị iche iche n'ụzọ dị iche iche na usoro mmeghachi omume dị mgbagwoju anya, yana mmụbawanye ngwa ngwa nwere ike iru 2Kb / min, nke dabara adaba maka mmezi Direct PCR.
-
Osisi akwukwo Direct PCR Plus kit
Ngwaahịa a na-eji usoro nchekwa lysis pụrụ iche iji kpochapụ ahịhịa osisi. Enwere ike iji lysate dị ka ndebiri na-enweghị ọcha. Mgbe ịtinye ihe mbido, enwere ike iji PCR Mix nke kit a maka mmụba.
-
Mkpụrụ Osisi Direct PCR Kit M / II
Ngwa a na-eji sistemụ lysis pụrụ iche iji wepụta mkpụrụ osisi. Enwere ike iji lysate dị ka ndebiri na-enweghị ọcha. Mgbe ịtinye ihe mbido, enwere ike iji PCR Mix nke kit a maka mmụba.
-
Osisi akwukwo Direct PCR tinyere kit-UNG
Dabere na Osisi Leaf Direct PCR Plus kit, a na-eji dUTP kama dTTP, na UNG enzyme nke nwere ike iweda template nwere dUTP. N'ụzọ dị otú a, a na-ahụpụta nkọwapụta na izi ezi nke mmụba, na nsogbu nke mmetọ ngwaahịa PCR nke nwere ike ịpụta n'oge nnukwu mkpụrụ ndụ ihe nketa na-egbochi.
-
Osisi akwukwo Direct PCR kit-UNG
Dabere na Osisi Leaf Direct PCR kit, a na-eji dUTP kama dTTP, na UNG enzyme nke nwere ike iweda template nwere dUTP. N'ụzọ dị otú a, a na-ahụpụta nkọwapụta na izi ezi nke mmụba, na nsogbu nke mmetọ ngwaahịa PCR nke nwere ike ịpụta n'oge nnukwu mkpụrụ ndụ ihe nketa na-egbochi.
