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Usoro iche iche RNA

Usoro nkewapụ DNA

Direct RT-qPCR usoro

Usoro ọbara kpọmkwem PCR

Osisi kpọmkwem PCR usoro

Anụmanụ kpọmkwem PCR usoro

Usoro PCR/RT-PCR

IVD akụrụngwa

Obere ngwa DNA

Mee ngwa ngwa DNA genomic dị elu site na ọbara anticoagulated (<1ml).

◮Enweghị mmetọ RNase:Ogidi DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase exogenous metọọ.

◮Ọsọ ọsọ:Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;arụ ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 20-80.

◮Dị mma:A na-eme centrifugation na okpomọkụ nke ụlọ, ọ dịghịkwa mkpa maka 4 Celsius C obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA.

◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.

◮Ịdị mma Dị elu:DNA genomic nke ewepụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe nyocha dị iche iche chọrọ.

◮Sistemụ Micro-elution:Ọ nwere ike ịbawanye ntinye nke DNA genomic, nke dị mma maka nchọpụta ala ma ọ bụ nnwale.

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General Plasmid Mini Kit General Plasmid DNA Extraction Miniprep Mini Kits

Mee ngwa ngwa dị ọcha DNA plasmid dị elu site na nje bacteria ndị gbanwere maka nyocha ihe ọmụmụ ihe ndụ dị ka mgbanwe na mgbaze enzyme.

◮Wepu RNA na-agbakwunyeghị RNase

◮Dị mma- A na-eme centrifugation na ụlọ okpomọkụ

◮Ngwa ngwa- Enwere ike ịmecha ọrụ ahụ na nkeji 20

◮Nchekwa- ọ dịghị organic reagent eji

◮Ịdị ọcha dị elu— OD260/280≈1.7-1.9

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RT Easyᵀᴹ I Master Premix maka njikọ cDNA nke mbụ

- Sistemụ ntụgharị ntụgharị nke ọma, ọ na-ewe naanị nkeji iri abụọ na ise iji mechaa njikọ nke eriri cDNA mbụ.

- Sistemụ ntụgharị ntụgharị uche dị elu, ndebiri ọkwa pg nwekwara ike nweta cDNA dị elu.

- Usoro ntụgharị ntụgharị nwere nkwụsi ike dị elu, okpomọkụ mmeghachi omume nke sistemụ nwere ike ịdị elu dị ka 50 ℃,ma ọ nwere ezigbo ọrụ ntụgharị ntụgharị.

- Ọbụna ụdị RNA adịghị ọcha (mmanya ruo 45%, nnu guanidine ruo 750mM) nwere ike idowe mmeghachi omume ntụgharị.

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RT Easy II (ya na gDNase) Master Premix maka njikọ cDNA nke mbụ maka PCR na gDNase

-Ike dị mma iwepu gDNA, nke nwere ike wepu gDNA na ndebiri n'ime nkeji 2.

Sistemụ ntụgharị ntụgharị nke ọma, ọ na-ewe naanị nkeji iri na ise iji mechaa njikọ nke eriri cDNA mbụ.

-Edebere mgbagwoju anya: ndebiri nwere ọdịnaya GC dị elu yana usoro nke abụọ dị mgbagwoju anya nwekwara ike iji ọrụ dị elu tụgharịa.

Sistemụ ntụgharị ntụgharị uche dị elu, ndebiri pg nwere ike nweta cDNA dị elu.

- Usoro ntụgharị ntụgharị nwere nkwụsi ike dị elu, ezigbo mmeghachi omume okpomọkụ bụ 42℃, ma ọ ka na-arụ ọrụ ntụgharị ntụgharị dị mma na 50℃.

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Real Time PCR Easyᵀᴹ-SYBR Green I kit

Ngwakọta dị mfe-2X PCR iji belata njehie nnwale na oge ọrụ

Ihe nchekwa akọwapụtara nke ọma na mmalite Taq enzyme nwere ike igbochi nkwalite na-abụghị nke akọwapụtara yana nguzobe primer dimer.

Mmetụta dị elu-nwere ike ịchọpụta obere ndebiri nke ndebiri

Ọdịiche dị mma—dakọtara na ngwa PCR kacha adị adị

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Ngwakọta Mkpokọta RNA Mkpokọta Anụmanụ Ngụkọta Mwepụta RNA na Ngwa Nsacha maka anụ ahụ anụmanụ & cell

Ngwa ngwa na ịrụ ọrụ nke ọma wepụta mkpokọta RNA ịdị ọcha na ịdị elu dị elu site na anụ ahụ anụmanụ dị iche iche.

Ọ dịghị mkpa ichegbu onwe gị maka mbibi RNA.Sistemụ a niile enweghị RNase

Wepu DNA nke ọma site na iji kọlụm DNA- Cleaning

Wepu DNA na-agbakwunyeghị DNAse

Mfe-arụ ọrụ niile na-arụcha na ụlọ okpomọkụ

Ngwa ngwa-enwere ike ịrụcha ọrụ n'ime nkeji iri atọ

Safe — ọ dịghị organic reagent eji

Ịdị ọcha dị elu-OD260/280≈1.8-2.1

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Ngwakọta mkpokọta cell RNA kewapụ ngwa ngwa RNA dị ọcha site na cell

Enwere ike nweta mkpokọta RNA sachachara nke ọma na nke dị elu site na sel dị iche iche emebere n'ime nkeji iri na otu.

RNase-efu

Na-arụ ọrụ na ụlọ okpomọkụ (15-25 ℃)

Site na kọlụm na-ehicha DNA

Mkpụrụ RNA dị elu

Ngwa ngwa: Mechaa mmịpụta n'ime nkeji iri na otu

Nchekwa: Ọ nweghị kemịkalụ Organic

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Ngwa Midi DNA nke ọbara (1-5ml) Ngwa Midi kewapụrụ ọbara maka DNA sitere na Ọbara

Mee ngwa ngwa genomic DNA dị elu site na ọbara anticoagulated (1-5ml).

◮Enweghị mmetọ RNase:Ogidi DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase exogenous metọọ.

◮Ọsọ ọsọ:Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;arụ ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 20-80.

◮Dị mma:A na-eme centrifugation na okpomọkụ nke ụlọ, ọ dịghịkwa mkpa maka 4 Celsius C obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA.

◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.

◮Ịdị mma Dị elu:DNA genomic nke ewepụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe nyocha dị iche iche chọrọ.

◮Sistemụ Micro-elution:Ọ nwere ike ịbawanye ntinye nke DNA genomic, nke dị mma maka nchọpụta ala ma ọ bụ nnwale.

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Buccal Swab/FTA Kaadị DNA kewapụ Kit Genomic DNA mmịpụta ma ọ bụ ihe dị ọcha sitere na Buccal Swabs

Mee ngwa ngwa DNA genomic dị elu site na nlele kaadị swab/FTA.

◮Enweghị mmetọ RNase:Ogidi DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase exogenous metọọ.

◮Ọsọ ọsọ:Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;arụ ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 20-80.

◮Dị mma:A na-eme centrifugation na okpomọkụ nke ụlọ, ọ dịghịkwa mkpa maka 4 Celsius C obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA.

◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.

◮Ịdị mma Dị elu:DNA genomic nke ewepụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe nyocha dị iche iche chọrọ.

◮Sistemụ Micro-elution:Ọ nwere ike ịbawanye ntinye nke DNA genomic, nke dị mma maka nchọpụta ala ma ọ bụ nnwale.

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Sistemụ njirimara DNA nke mbụ 27Y

MbidoSistemụ njirimara DNA 27Y na-eji teknụzụ akara fluorescent nwere agba isii iji gbasaa 27 Y-STR loci n'otu oge.Gụnyere: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS448, DYS533, DYFY387 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.

Ngwa ahụ nwere ikike mgbochi mgbochi dị mma, ma nwee ike ịbawanye akwụkwọ nzacha ozugbo, kaadị FTA, swab owu, kaadị saliva, swab ọnụ, yana enwere ike iji wepụ ma sachaa ndebiri DNA site na ihe ikpe.

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Sistemụ njirimara DNA nke mbụ 38Y-C (Ikpe)

MbidoSistemụ njirimara DNA 38Y-C na-eji teknụzụ akara fluorescent nwere agba isii iji bulie 38 Y chromosome STR mpaghara, 3 Y-Ebe obibinaabụọ esịtidem mma akara loci n'otu ogemaka nkwalite Nlele ikpe ewepụtara na ndebiri DNA emere ka ọ dị ọcha.

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Sistemụ njirimara DNA nke mbụ 30A (anaghị amịpụta)

Sistemụ njirimara DNA FOREGENE 30A na-eji teknụzụ akara fluorescent agba isiigbasaa 29autosomalSTR ebe, otuMpaghara Amel na otu mpaghara Yindel n'otu oge, ma nwee ike ịkwalite akwụkwọ nzacha ozugbo, FTAkaadị, owu swabs, kaadị saliva,na mpụọ swab.

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