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Ké ọdụ Direct PCR Kit
Ngwa a na-eji sistemụ lysis pụrụ iche iji wepụta DNA genomic ngwa ngwa na ọdụ, ntị, mọzụlụ na anụ ahụ ndị ọzọ maka mmeghachi omume PCR, yabụ ọ dabara adaba maka nnwale mkpụrụ ndụ buru ibu.
Usoro nke ịhapụ DNA genomic site na nchekwa lysis ka emechara n'ime 10-30 nkeji na 65 Celsius. Enweghị usoro ndị ọzọ dị ka protein na mwepụ RNA achọrọ, na DNA nwere ike iji dị ka ndebiri maka mmeghachi omume PCR.
2 × M1-PCR Easy TM Mix nwere nnagide siri ike nye ndị mmechi mmeghachi omume PCR, ma nwee ike iji lysate nke nlele ahụ nwalee ya dị ka ndebiri maka mmụba nke ọma na nke a kapịrị ọnụ. Ihe nyocha a nwere Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, nchekwa nchekwa, PCR optimizer na stabilizer.
D-Taq DNA polymerase bụ DNA polymerase nke mepụtara nke Foregene maka mmeghachi omume PCR kpọmkwem. D-Taq DNA polymerase nwere nnagide siri ike nye ọtụtụ ndị mmeghachi omume PCR, ma nwee ike ịkọwapụta DNA dị iche iche n'ụzọ dị iche iche na usoro mmeghachi omume dị mgbagwoju anya, yana mmụbawanye ngwa ngwa nwere ike iru 2Kb / min, nke dabara adaba maka mmezi Direct PCR.
