China Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mouse Tail)(maka Genotyping) Onye nrụpụta na onye na-eweta |Mbido

Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mouse Tail)(maka Genotyping)

Nkọwa ngwa:

Jiri lysate ọdụ òké ozugbo dị ka ndebiri maka nkwalite PCR, na-enweghị mmịpụta DNA dị iche iche, ngwa ngwa na nghọta dị elu.

◮Ọ dịghị DNA na-ewe oge na ọnụ ahịa ọcha achọrọ.

◮Ọchịchọ nlele dị ntakịrị, were naanị otu mkpụrụ.

◮Ọ dịghị ọgwụgwọ pụrụ iche dị ka egweri na ịkụpịa ihe a chọrọ, na ọrụ dị mfe.

◮Usoro PCR kachasị mma na-enyere PCR aka inwe nkọwa dị elu yana nnabata siri ike nye ndị na-egbochi mmeghachi omume PCR.

Zitere anyị email

Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwapụta

Ngwa a na-eji sistemu nchekwa lysis pụrụ iche iji wepụta DNA genomic ngwa ngwa site na ọdụ òké na ihe nlele ntị òké maka mmeghachi omume PCR, yabụ na ọ dabara adaba maka nnwale mkpụrụ ndụ ihe nketa buru ibu.

Usoro nke ịtọhapụ DNA genomic site na nchekwa lysis agwụlan'ime 10-30 nkejinaokpomọkụ (20-25).Celsius C).Ọ dịghị kpo oku a chọrọ, na usoro ndị ọzọ dị ka protein na RNA mwepụ adịghị mkpa.Enwere ike iji ọnụọgụ DNA ewepụtara dị ka Template maka mmeghachi omume PCR.

2 × M-PCR dị mfe^TMNgwakọta nwere nnabata siri ike na ndị na-egbochi mmeghachi omume PCR, ma nwee ike iji lysate nke ihe nlele a nwalere dị ka ndebiri maka nkwalite dị mma na nke akọwapụtara.Nke a reagent nwere Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, ihe nchekwa mmeghachi omume, PCR optimizer na stabilizer.

D-Taq DNA polymerase bụ DNA polymerase nke Foregene mepụtara maka mmeghachi omume PCR kpọmkwem.D-Taq DNA polymerase nwere nnabata siri ike maka ọtụtụ ihe mgbochi mmeghachi omume PCR, ma nwee ike ịbawanye ọnụọgụ DNA nke ọma na sistemụ mmeghachi omume dị mgbagwoju anya, yana ọsọ nkwalite nwere ike iru 2Kb / min, nke dabara adaba maka mmeghachi omume PCR Direct.

Nkọwapụta

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ngwa ngwa

Akụkụ I	Buffer NP
	Foregene Protease Plus I
	Ihe nchekwa 6 × DNA na-ebufe
Akụkụ II	2× M PCR EasyTM Mix
Ntuziaka

Atụmatụ & uru

■Ọ dịghị DNA na-ewe oge na ọnụ ahịa ọcha achọrọ.

■ Ihe nlele achọrọ dị ntakịrị, were naanị ọdụ òké (ma ọ bụ ntị òké nwere dayameta nke 2-5mm) karịa 2mm.

∎ Ọ dị mfe iji rụọ ọrụ, enwere ike ịsa ya na-enweghị kpo oku iji mee ka protease rụọ ọrụ, na ngwaahịa lysed nwere ike itinye ya ozugbo na mmeghachi omume PCR.

■ Enwere ike iji Kit Foregene Mouse Tail Genomic DNA Extraction Kit (DE-05211) wepụ DNA genomic n'ọdụ oke a na-ahazi.

∎ Sistemu PCR kachasị mma na-eme ka PCR nwee nkọwa dị elu yana nnabata siri ike nye ndị na-egbochi mmeghachi omume PCR.