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Kedu ihe dị iche n'etiti PCR, RT-PCR, qPCR na RT-qPCR
PCR bụ usoro eji eme ka DNA mụbaa site na obere ndebiri DNA.RT-PCR na-eji ntụgharị ntụgharị iji mepụta ndebiri DNA sitere na isi iyi RNA nke enwere ike ịbawanye elu.PCR na RT-PCR na-abụkarị mmeghachi omume njedebe, ebe qPCR na RT-qPCR na-eji kinetics nke ọnụego nke synt ngwaahịa ...GỤKWUO -
Ihe dị n'ihu nke nchọpụta ọgwụ: Iji obere ụmụ irighiri amụba RNA
Ọgwụ mgbochi mRNA nke Pfizer maka COVID ewelitela mmasị nke iji ribonucleic acid (RNA) dị ka ebumnuche ọgwụgwọ.Otú ọ dị, iji obere ụmụ irighiri ihe echere RNA bụ ihe ịma aka nke ukwuu.RNA nwere naanị ihe mgbochi anọ: adenine (A), cytosine (C), guanine (G), na uracil (U) nke nọchiri anya thymin ...GỤKWUO -
Kedu ihe bụ ọgwụ nucleic acid?
"Nucleic acid ọgwụ" na-eji "nucleic acid," nke na-ezo aka n'ihe ndị dị ka DNA na RNA na-achịkwa ozi mkpụrụ ndụ ihe nketa, dị ka ọgwụ.Ndị a na-enye ohere ichebe ụmụ irighiri ihe dị ka mRNA na miRNA nke enweghị ike iji ọgwụ ọdịnala dị ala dị ala na ọgwụ mgbochi mmadụ, yana enwere g...GỤKWUO -
Ahụmahụ ụfọdụ gbasara siRNA na RT-PCR
1. Basic ihe ọmụma (ma ọ bụrụ na ị chọrọ ịhụ ibuo akụkụ, biko nyefee ozugbo na nke abụọ akụkụ) Dị ka a ewepụtara mmeghachi omume nke ot PCR, Real oge PCR tumadi Monitors mgbanwe nke ego nke amplification ngwaahịa na onye ọ bụla okirikiri nke PCR amplification. mmeghachi omume ozugbo ...GỤKWUO -
Usoro njikarịcha usoro nzaghachi nnwale RT-PCR zuru oke
一, Mụbaa uche nke mmeghachi omume usoro: 1. Wepụ elu àgwà RNA: Ịga nke ọma cDNA njikọ sitere na elu-edu RNA.RNA dị elu kwesịrị opekata mpe bụrụ ogologo zuru oke yana enweghị ihe mgbochi transcriptase dị ka EDTA ma ọ bụ SDS.Ogo nke RNA na-ekpebi kacha a...GỤKWUO -
Nhazi primer PCR zuru oke yana nkọwa PCR
Nhazi nhazi nke izizi (enwere ike idozi nsogbu 99%) 1. Ogologo oge mbụ: Akwụkwọ ọgụgụ chọrọ 15-30bp, na-abụkarị ihe dịka 20bp.Ọnọdụ dị n'ezie ka mma ịbụ 18-24bp iji hụ na nkọwapụta, mana ogologo oge ka mma, ogologo primer ga-ebelata nkọwapụta, ma belata mkpụrụ.2. Nke mbụ...GỤKWUO -
Akpachara anya maka qRT-PCR nke agadi Dọkịta chịkọtara nke ọma
Onye ọ bụla na-ekwu maka ụkpụrụ nke nnwale qRT-PCR, nhazi mbụ, nkọwa nsonaazụ, wdg, mana echere m na m ga-eso gị kerịta ọrụ nnwale nke qRT-PCR.Ọ dị obere, mana ọ gbasara nsonaazụ.Tupu anyị emee qRT-PCR, anyị kwesịrị inwe nghọta doro anya banyere…GỤKWUO -
Akpachara anya maka RT-qPCR
Nnwale RT-qPCR gụnyere mmịpụta RNA na nyocha ịdị mma, ntụgharị ntụgharị na qPCR nzọụkwụ atọ, usoro nke ọ bụla nwere ọtụtụ ịkpachara anya, anyị ga-ewebata n'ụzọ zuru ezu n'okpuru.ⅠNtụle ogo RNA na nnwale RT-qPCR, ka emechara mmịpụta RNA, ...GỤKWUO -
Okwu mmeghe nke PCR nke ọma
1. Nghọta mmalite N'oge a, anyị kwesịrị ịghọta ụfọdụ echiche na okwu okwu, ka anyị wee zere imehie ihe n'ihu ndị agadi anyị, dị ka: Ajụjụ: Gịnị bụ ọdịiche dị n'etiti RT-PCR, qPCR, PCR Real-time, na ezigbo oge RT-PCR?Azịza: RT-PCR bụ PCR ntụgharị ntụgharị (atụgharị t...GỤKWUO -
PCR, otutu PCR, N'ebe PCR, Reverse PCR, RT-PCR, qPCR (1) - PCR
PCR, multiple PCR, In situ PCR, Reverse PCR, RT-PCR, qPCR (1) - PCR Anyị ga-edozi echiche, nzọụkwụ na nkọwa nke dị iche iche PCR Ⅰ.PCR Polymerase Chain Reaction, nke a na-akpọ PCR, bụ nkà na ụzụ nke ndụ ndụ nke a na-eji amụba iberibe DNA kpọmkwem.Enwere ike were ya dị ka ...GỤKWUO -
ụkpụrụ nnwale qRT-PCR
Emepụtara RT-qPCR site na teknụzụ PCR nkịtị.Ọ na-agbakwunye kemịkalụ fluorescent (ụcha fluorescent ma ọ bụ nyocha fluorescent) na sistemu mmeghachi omume PCR ọdịnala, wee chọpụta usoro mbelata PCR na ndọtị ozugbo dị ka usoro luminescent ha dị iche iche si dị.Akara fluorescent...GỤKWUO -
Usoro njikarịcha usoro nzaghachi nnwale RT-PCR zuru oke
ⅠMụbaa mmetụta nke usoro mmeghachi omume: 1. Kewapụ RNA dị elu dị elu: Nchịkọta cDNA na-aga nke ọma sitere na RNA dị elu.RNA dị elu kwesịrị ịhụ na opekata mpe ogologo oge na enweghị ihe mgbochi na-enweghị enzymes ndekọ, dị ka EDTA ma ọ bụ SDS.The qual...GỤKWUO
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