N'oge mmalite nke ntiwapụ ahụ, n'ihi mmepe ngwa ngwa, nyocha ngwa ngwa nke ndị ọrịa a na-enyo enyo bụ isi ihe na-egbochi COVID-19.Ụfọdụ reagents nchọpụta nucleic acid akwadoro nwere obere oge mmepe, yana enwere nsogbu dịka nkwenye arụmọrụ ngwa ngwa, njikarịcha reagent ezughi oke, na nnukwu ọdịiche dị n'etiti batches;Nsogbu nke ụlọ nyocha ụlọ ọgwụ dị iche iche na akụkụ dị iche iche nke usoro nchọpụta nucleic acid nwekwara ike imetụta izi ezi nke nchọpụta nucleic acid.Edemede a ga-elekwasị anya na isi njikọ na isi ihe dị na nchọpụta nucleic acid SARS-CoV-2 ugbu a, wee nyochaa nsogbu nke nyochaghachi nke ọma na nke ọma nke nchọpụta nucleic acid ụlọ nyocha na enweghị nkwekọrịta ụlọ ọgwụ.

Ụkpụrụ nke nchọpụta SARS-CoV-2 nucleic acid

SARS-CoV-2 bụ nje RNA nwere usoro genome nke ihe dịka 29 kb, nwere mkpụrụ ndụ ihe nketa iri, nke nwere ike itinye protein 10 nke ọma.Ihe mejupụtara nje bụ RNA na protein, na oyi akwa dị n'elu bụ mkpuchi mpụta nke lipids na glycoproteins mejupụtara.N'ime ya, capsid protein na-ekechi RNA na ya, si otú ahụ na-echebe RNA (P1) dị mfe imebi.

Ọdịdị P1 nke SARS-COV-2

Nje Virus na-abanye n'ime sel site na ndị na-anata elu sel kpọmkwem iji bute ọrịa, ma na-eji sel ndị ọbịa na-emepụtaghachi.

Ụkpụrụ nke nchọpụta nucleic acid viral bụ ikpughe nje RNA site na cell lysate, wee jiri ezigbo oge fluorescent reverse transcription-polymerase chain reaction (RT-PCR) maka nchọpụta.

Isi ihe na ụkpụrụ nchọpụta bụ iji primers na nyocha iji nweta "ezubere iche dakọtara" nke usoro nucleic acid, ya bụ, ịchọta usoro nucleic acid nke SARS-CoV-2 nke dị iche na nje ndị ọzọ na ihe dịka 30,000 bases (myirịta nke nucleic acid na nje ndị ọzọ) "Low" mpaghara), ndị na-emepụta ihe na ndị na-emepụta ihe.

Ihe ndị na-emepụta ihe na nyocha dabara nke ọma na mpaghara SARS-CoV-2 nucleic acid, ya bụ, nkọwapụta siri ike.Ozugbo ezigbo fluorescent RT-PCR amplification nsonaazụ nlele a ga-anwale dị mma, ọ na-egosi na SARS-CoV-2 dị na nlele ahụ.Lee P2.

Nzọụkwụ P2 nke SARS-CoV-2 nucleic acid mkpebi siri ike (ezigbo fluorescent RT-PCR)

Ọnọdụ na ihe achọrọ nke ụlọ nyocha maka nchọpụta SARS-CoV-2 nucleic acid

Ụlọ nyocha nyocha nke Nucleic acid bụ ihe kachasị mma maka gburugburu nrụgide na-adịghị mma, na ha kwesịrị ịṅa ntị na nlekota nrụgide, na-eme ka ikuku na-eru, ma wepụ ikuku aerosols.Ndị na-ahụ maka ule Nucleic acid ga-enwerịrị ntozu ndị kwekọrọ, nata ọzụzụ mmeghachi omume polymerase dị mkpa ma gafere nyocha ahụ.Ekwesịrị ijikwa ụlọ nyocha ahụ nke ọma, kechie ya ebe, machibido ndị ọrụ na-adịghị mkpa ịbanye.Ebe dị ọcha kwesịrị ka ikuku na-ekpochapụ ya ma kpochapụ ya.A na-etinye ihe ndị dị mkpa na mpaghara, a na-ekewapụ ihe dị ọcha na nke ruru unyi, dochie ya n'oge, ma metọọ ya n'ebe.Ngwọta a na-eme kwa oge: ọgwụ mgbochi nwere chlorine bụ isi ihe ngwọta maka mpaghara buru ibu, enwere ike iji mmanya 75% mee obere mpaghara.Ụzọ dị mma isi mee ihe banyere ikuku ikuku bụ imeghe windo maka ikuku ikuku, a pụkwara ime nsị ikuku site na ụzarị ultraviolet, filtration, na nsị ikuku.

Isi njikọ na oke nke SARS-CoV-2 nucleic acid mkpebi (ezigbo fluorescent RT-PCR)

Ọ bụ ezie na ụlọ nyocha n'ozuzu na-aṅa ntị nke ọma na nucleic acid "nchọpụta", n'ezie, nucleic acid "mwepụta" bụkwa otu n'ime usoro ndị dị mkpa maka nchọpụta ihe ịga nke ọma, nke nwere njikọ chiri anya na nchịkọta na nchekwa nke ihe nlele nje.

Ka ọ dị ugbu a, ihe nlere iku ume ndị a na-ejikarị eme ihe, dị ka nasopharyngeal swabs, na-eji usoro nke abụọ, nke bụ ihe nkwụsị (nchekwa) ngwọta nke a kwadebere na-adabere na mmịpụta nucleic acid na ngwọta lysis.N'otu aka ahụ, ihe ngwọta nchekwa nje a nwere ike denature protein nke nje ahụ, hapụ ọrụ ya ma gharakwa ibufe ọrịa, ma melite nchekwa nke njem na nchọpụta ogbo;N'aka nke ọzọ, ọ nwere ike ịgbawa nje virus ozugbo iji wepụta nucleic acid, wepụ enzyme nucleic acid na-emebi emebi, ma gbochie nje ahụ.A na-emebi RNA.

Ihe ngwọta nlele nje akwadoro na ndabere nke nucleic acid extract lysis solution.Isi ihe ndị bụ isi bụ salts kwesịrị ekwesị, ethylenediaminetetraacetic acid chelating agent, guanidine nnu (guanidine isothiocyanate, guanidine hydrochloride, wdg), anionic surfactant (dodecane) Sodium sulfate), cationic surfactant (tetradecyl trimethyl ammonium oxalate), phenol, 8-hydroksyreline ma ọ bụ protein oxide moreKa ọ dị ugbu a, e nwere ọtụtụ ụdị ngwa mịpụta nucleic acid, a na-ejikwa ihe dị iche iche nke mmịpụta nucleic acid na reagents dị ọcha.Ọ bụrụgodị na a na-eji otu mmịpụta nucleic acid na reagent dị ọcha, usoro mmịpụta nke ngwa ọ bụla dị iche.

Ka ọ dị ugbu a, a na-ahọrọ ngwaahịa nchọpụta nucleic acid nke National Medical Product Administration kwadoro dabere na mkpụrụ ndụ ORF1ab, E na N na genome SARS-CoV-2.Ụkpụrụ nchọpụta nke ngwaahịa dị iche iche bụ otu n'ime isi, ma primers ha na nyocha nyocha dị iche iche.Enwere ngalaba ebumnuche otu (ORF1ab), akụkụ abụọ ebumnuche (ORF1ab, N ma ọ bụ E), yana akụkụ atọ (ORF1ab, N na E).Ọdịiche dị n'etiti nchọpụta na nkọwa, mmịpụta nucleic acid na ezigbo fluorescent RT-PCR mmeghachi omume kwesịrị izo aka na ntuziaka ngwa dị mkpa, a na-atụ aro ka ndị ọrụ gbasoo usoro nkọwa akọwapụtara na ntuziaka ngwa maka nkọwa.Mpaghara a na-ahụkarị, primers na usoro nyocha agbagoro site na ezigbo fluorescent RT-PCR na-egosi na P3.

P3 Ọnọdụ SARS-CoV-2 amplicon lekwasịrị anya na genome na usoro nke primers na nyocha.

Nkọwa nke nsonaazụ SARS-CoV-2 nucleic acid mkpebi siri ike (Rala-Time fluorescent RT-PCR)

"Atụmatụ Mgbochi na Mgbochi Ọrịa oyi baa maka ọrịa SARS-CoV-2 (mbibi nke abụọ)" na nke mbụ kọwara ụkpụrụ maka ikpebi nsonaazụ otu mkpụrụ ndụ ihe nketa:

1. Ọ dịghị Ct ma ọ bụ Ct≥40 adịghị mma;

2. Ct <37 dị mma;

3. Uru Ct nke 37-40 bụ mpaghara isi awọ.A na-atụ aro ka ịmegharị nnwale ahụ.Ọ bụrụ na nsonaazụ nke ịmegharị Ct <40 na usoro nkwalite ahụ nwere ọnụ ọgụgụ doro anya, a na-ekpebi ihe nlele ahụ dị ka nke ọma, ma ọ bụghị na ọ dị njọ.

Mbipụta nke atọ nke ntuziaka na mbipụta nke anọ nke ntuziaka ahụ gara n'ihu na njirisi dị n'elu.Agbanyeghị, n'ihi ebumnuche dị iche iche ejiri na ngwa azụmaahịa, mbipụta 3 nke e kwuru n'elu nke ntuziaka enyeghị njirisi maka ikpebi nchikota ebumnuche, na-ekwusi ike ntuziaka ndị nrụpụta nyere ga-emeri.Malite na mbipụta nke ise nke ụkpụrụ nduzi, a kọwapụtala ebumnuche abụọ, karịsịa usoro ikpe maka otu ebumnuche nke siri ike ikpe.Ya bụ, ọ bụrụ na ụlọ nyocha chọrọ igosipụta na ikpe dị mma maka nchọpụta SARS-CoV-2 nucleic acid, ihe ndị a kwesịrị imezu ọnọdụ 1 n'ime 2:

(1) A na-anwale ebumnuche abụọ nke SARS-CoV-2 (ORF1ab, N) n'otu nlele site na ezigbo fluorescent RT-PCR.Ọ bụrụ na otu ebumnuche dị mma, a ga-achọ nyochagharị na nyocha ọzọ.Ọ bụrụ na nsonaazụ ule ahụ dị Ọ bụrụ na otu ebumnuche ka dị mma, a na-ewere ya dị ka ihe dị mma.

(2) Ihe nlele abụọ nke ezigbo fluorescent RT-PCR gosipụtara otu ebumnuche dị mma n'otu oge ma ọ bụ ihe nlele abụọ nke otu ụdị gosipụtara otu nsonaazụ nyocha dị mma, nke enwere ike ikpebi dị ka ihe dị mma.Agbanyeghị, ntuziaka a na-ekwusi ike na nsonaazụ ọjọọ nke nnwale nucleic acid enweghị ike wepu ọrịa SARS-CoV-2.Ihe ndị nwere ike ime ka ihe ndị na-adịghị mma dị mkpa ka ewepụ ya, gụnyere àgwà adịghị mma (ntụgharị iku ume sitere na oropharynx na akụkụ ndị ọzọ), nchịkọta ihe nlele n'oge ma ọ bụ n'oge na-adịghị anya, A naghị echekwa ihe nlele, bufee, na nhazi nke ọma, na nkà na ụzụ n'onwe ya nwere nsogbu (ngbanwe nje, mgbochi PCR), wdg.

Ihe na-akpata adịghị mma na nchọpụta SARS-CoV-2

Echiche nke "ụgha ụgha" na ule nucleic acid nke a na-eche ugbu a, na-ezokarị aka na "adịghị mma" nke nsonaazụ ule nucleic acid na-ekwekọghị na ngosipụta ụlọ ọgwụ, ya bụ, mgbaàmà ụlọ ọgwụ na nsonaazụ onyonyo bụ nke a na-enyo enyo nke COVID-19, mana ule nucleic acid na-abụkarị "adịghị mma" ọtụtụ oge.Clinical Laboratory Center nke National Health Commission kọwara nnwale SARS-CoV-2 nke “adịghị mma”.

(1) Enwere ụfọdụ nje n'ime mkpụrụ ndụ nke onye bu ọrịa ahụ.Ihe data dị adị na-egosi na mgbe nje butere ahụ ahụ, nje a na-esi na imi na ọnụ na-abanye n'akpịrị, wee banye trachea na bronchi, wee rute alveoli.Onye butere ọrịa ahụ ga-enweta oge nnabata, mgbaàmà dị nro, yana usoro nke mgbaàmà siri ike, yana ọkwa dị iche iche nke ọrịa ahụ.Na ọnụọgụ nje dị n'akụkụ dị iche iche nke ahụ dị iche.

N'ihe gbasara ibu viral nke ụdị cell, alveolar epithelial cell (obere akụkụ iku ume tract)> ikuku epithelial cell (n'elu iku ume tract)> fibroblasts, endothelial cell, na macrophages, wdg;site na ụdị ihe atụ, mmiri mmiri alveolar lavage (kacha mma)> sputum dị omimi> nasopharyngeal swab>oropharyngeal swab>ọbara.Tụkwasị na nke ahụ, a pụkwara ịhụ nje a na nsị.Otú ọ dị, n'ịtụle ịdị mma nke ọrụ na ịnakwere ndị ọrịa, usoro ihe nlele ahụike a na-ejikarị eme ihe bụ oropharyngeal swab>nasopharyngeal swab>bronchial lavage fluid (arụ ọrụ mgbagwoju anya) na sputum miri emi (na-emekarị ụkwara akọrọ, siri ike inweta) .

Ya mere, ọnụọgụ nje na sel nke oropharynx ma ọ bụ nasopharynx nke ụfọdụ ndị ọrịa dị ntakịrị ma ọ bụ dị oke ala.Ọ bụrụ na a na-ewere naanị ihe atụ nke oropharynx ma ọ bụ nasopharynx maka nnwale, a gaghị achọpụta nje nucleic acid.

(2) Ọ dịghị mkpụrụ ndụ nwere nje a chịkọtara n'oge a na-achịkọta ihe atụ, ma ọ bụ n'ime nje virus nucleic acid echekwaghị nke ọma.

[① Ebe nchịkọta na-ezighị ezi, dịka ọmụmaatụ, mgbe ị na-anakọta swabs oropharyngeal, omimi nchịkọta ezughị ezu, a naghị achịkọta swabs nasopharyngeal swabs n'ime oghere imi, wdg. Ọtụtụ n'ime mkpụrụ ndụ anakọtara nwere ike ịbụ mkpụrụ ndụ na-enweghị nje;

②A na-eji swabs sample eme ihe na-ezighi ezi.Dịka ọmụmaatụ, a na-atụ aro fibers sịntetik dị ka PE fiber, polyester fiber na polypropylene fiber maka ihe nke isi swab.A na-eji eriri eke dị ka owu na-arụ ọrụ n'ezie (protein siri ike na ọ dịghị mfe ịsacha) na eriri naịlọn (mmiri mmiri na-adịghị mma, na-eduga na oke nlele zuru oke);

③ Iji nje nchekwa tubes eme ihe n'ụzọ na-ezighi ezi, dị ka iji polypropylene ma ọ bụ polyethylene plastic nchekwa tubes dị mfe ịmịkọrọ acid nucleic (DNA/RNA), na-ebute mbelata nke nucleic acid na ngwọta nchekwa.Na omume, a na-atụ aro ka iji polyethylene-propylene polymer plastic na ụfọdụ arịa rọba polypropylene a na-emeso ya nke ọma iji chekwaa acid nucleic viral.]

[① Ebe nchịkọta na-ezighị ezi, dịka ọmụmaatụ, mgbe ị na-anakọta swabs oropharyngeal, omimi nchịkọta ezughị ezu, a naghị achịkọta swabs nasopharyngeal swabs n'ime oghere imi, wdg. Ọtụtụ n'ime mkpụrụ ndụ anakọtara nwere ike ịbụ mkpụrụ ndụ na-enweghị nje;

②A na-eji swabs sample eme ihe na-ezighi ezi.Dịka ọmụmaatụ, a na-atụ aro fibers sịntetik dị ka PE fiber, polyester fiber na polypropylene fiber maka ihe nke isi swab.A na-eji eriri eke dị ka owu na-arụ ọrụ n'ezie (protein siri ike na ọ dịghị mfe ịsacha) na eriri naịlọn (mmiri mmiri na-adịghị mma, na-eduga na oke nlele zuru oke);

③ Iji nje nchekwa tubes eme ihe n'ụzọ na-ezighi ezi, dị ka iji polypropylene ma ọ bụ polyethylene plastic nchekwa tubes dị mfe ịmịkọrọ acid nucleic (DNA/RNA), na-ebute mbelata nke nucleic acid na ngwọta nchekwa.Na omume, a na-atụ aro ka iji polyethylene-propylene polymer plastic na ụfọdụ arịa rọba polypropylene a na-emeso ya nke ọma iji chekwaa acid nucleic viral.]

(4) A naghị ahazi ọrụ ụlọ nyocha ụlọ ọgwụ.Nlereanya njem na ọnọdụ nchekwa, ọrụ ahaziri ahazi nke ụlọ nyocha ụlọ ọgwụ, nkọwa nsonaazụ na njikwa mma bụ isi ihe iji hụ na izi ezi na ntụkwasị obi nke nsonaazụ ule.Dabere na nsonaazụ nke nyocha ịdị mma mpụga nke Clinical Laboratory Center nke National Health Commission mere na Machị 16-24, 2020, nke ụlọ nyocha 844 nwetara nsonaazụ ziri ezi, 701 (83.1%) tozuru oke, yana 143 (16.9%) erughị eru.Ndị tozuru oke, ọnọdụ nnwale ụlọ nyocha n'ozuzu dị mma, mana ụlọ nyocha dị iche iche ka nwere ndịiche na ikike ọrụ ndị ọrụ, ikike nlegharị anya nlere otu ebumnuche na njikwa mma.

Kedu otu esi ebelata ihe ọjọọ nke SARS-CoV-2 nucleic acid nchọpụta?

Ibelata ihe na-adịghị mma na nchọpụta nucleic acid kwesịrị ka emeziwanye ya site na akụkụ anọ nke ịmepụta ihe ọjọọ.

(1) Enwere ụfọdụ nje n'ime mkpụrụ ndụ nke onye butere ọrịa ahụ.Nleba nje virus n'akụkụ dị iche iche nke ahụ ndị a na-enyo enyo na ọ ga-adị iche n'oge dị iche iche.Ọ bụrụ na enweghị pharynx, ọ nwere ike ịbụ na mmiri mmiri nke lavage bronchial ma ọ bụ nsị.Ọ bụrụ na enwere ike ịnakọta ọtụtụ ụdị ihe nlele n'otu oge ma ọ bụ na ọkwa dị iche iche nke ọganihu ọrịa maka nyocha, ga-enyere aka zere ihe ndị na-adịghị mma.

(2) Ekwesịrị ịnakọta mkpụrụ ndụ nwere nje n'oge anakọta ihe nlele.Enwere ike idozi nsogbu a ruo n'ókè dị ukwuu site n'ịkwalite ọzụzụ nke ndị nchịkọta ihe nlele.

(3) Reagents IVD ntụkwasị obi.Site na-eme nnyocha na nchọpụta arụmọrụ nke nyocha nke reagents na ọkwa mba, na-atụle nsogbu ndị dị ugbu a, a ga-eme ka nchọpụta nchọpụta nke reagents dịkwuo mma ma nwee ike imeziwanye mmetụta nke nyocha.

(4) Ọrụ nhazi nke ụlọ nyocha ụlọ ọgwụ.Site n'ịkwalite ọzụzụ nke ndị ọrụ ụlọ nyocha, na-aga n'ihu na-emeziwanye usoro nlekọta ụlọ nyocha ụlọ nyocha, na-ahụ maka nkewa ezi uche dị na ya, na imeziwanye ikike nke ndị ọrụ ịchọpụta, ọ ga-ekwe omume ibelata ihe ndị na-adịghị mma n'ihi ọrụ ụlọ nyocha na-ezighị ezi.

Ihe kpatara nyochaghachi nke ọma nke SARS-CoV-2 nucleic acid test n'ime ndị ọrịa gbakere na ewepụrụ.

“Atụmatụ nyocha na ọgwụgwọ COVID-19 (Nnwale nke asaa)” na-ekwupụta n'ụzọ doro anya na otu n'ime njirisi maka ndị ọrịa COVID-19 ga-agwọta ma wepụ ya n'ụlọ ọgwụ bụ na ihe nlele traktị iku ume abụọ na-esochi nwere ule nucleic acid na-adịghị mma (opekata mpe awa 24), mana enwere ole na ole The SARS-CoV-2 nucleic acid butere ọzọ n'ihi nnwale dị iche iche nke ndị ọrịa SARS.

(1)SARS-CoV-2 bụ nje ọhụrụ.Ọ dị mkpa ịghọtakwu usoro nje ya, foto zuru oke nke ọrịa kpatara na njirimara nke usoro ọrịa ahụ.Ya mere, n'otu aka ahụ, ọ dị mkpa iji mee ka nlekọta nke ndị ọrịa na-apụ apụ ma mee nchọpụta ahụike ụbọchị 14.Na-eme nleba anya, nlekọta ahụike na nduzi ahụike iji mee ka nghọta nke usoro dum nke ihe omume, mmepe na pụta ọrịa ahụ.

(2) Onye ọrịa ahụ nwere ike bute nje ahụ ọzọ.Ọkammụta Zhong Nanshan kwuru, sị: N'ihi na ndị ọrịa gwọrọ nwere ọgwụ mgbochi ọrịa, SARS-CoV-2 nwere ike iwepụ ya site na ọgwụ mgbochi mgbe ha wakporo ọzọ.Enwere ọtụtụ ihe kpatara ya, nke nwere ike bụrụ ihe kpatara onye ọrịa ahụ gbakere, ma ọ bụ ọ nwere ike jikọta ya na ngbanwe nke nje, ma ọ bụ ọbụna ihe kpatara nyocha ụlọ nyocha.Ọ bụrụ na ọ bụ nje ahụ n'onwe ya, mgbanwe SARS-CoV-2 nwere ike ime ka ọgwụ mgbochi nke onye ọrịa gbakere ghara ịdị irè megide nje mutated.Ọ bụrụ na onye ọrịa ahụ butere nje virus mutated ọzọ, nnwale nucleic acid nwere ike ịdị mma ọzọ.

(3) N'ihe gbasara usoro nyocha ụlọ nyocha, usoro nyocha ọ bụla nwere oke ya.Nchọpụta SARS-CoV-2 nucleic acid bụ n'ihi nhọrọ nke usoro mkpụrụ ndụ ihe nketa, ihe mejupụtara reagents, ikike nke usoro ahụ na ihe ndị ọzọ, na-eduga na ngwa ndị dị ugbu a nwere oke nchọpụta dị ala.Mgbe a gwọchara onye ọrịa ahụ, nje dị n'ime ahụ na-ebelata.Mgbe ibu nje dị na nlele a ga-anwale dị n'okpuru njedebe nke nchọpụta, nsonaazụ "adịghị mma" ga-apụta.Agbanyeghị, nsonaazụ a apụtaghị na nje dị n'ime ahụ apụọla kpamkpam.Nje nwere ike ịbụ mgbe a kwụsịrị ọgwụgwọ ahụ.Mwelite”, gaa n'ihu na-eṅomi.Ya mere, a na-atụ aro ka nyochaa otu ugboro n'izu n'ime izu 2 ruo 4 mgbe a kwụsịrị.

(4) Nucleic acid bụ mkpụrụ ndụ ihe nketa nke nje.A na-egbu nje ahụ mgbe a gbasịrị onye ọrịa ahụ ọgwụgwọ antiviral, mana iberibe RNA viral nke fọdụrụ ka na-edobe n'ahụ mmadụ, a naghị ewepụ ya kpamkpam n'ahụ.Mgbe ụfọdụ, n'okpuru ọnọdụ ụfọdụ, enwere ike idowe ya karịa.Ogologo oge, na n'oge a ule nucleic acid ga-abụ "na-agafe agafe" nke ọma.Site na ịgbatị oge mgbake nke onye ọrịa, mgbe iberibe RNA ndị fọdụrụ n'ime ahụ ji nwayọọ nwayọọ gwụ, nsonaazụ ule nucleic acid nwere ike gbanwee na-adịghị mma.

(5) Nsonaazụ ule nucleic acid nke SARS-CoV-2 na-egosipụta naanị ọnụnọ ma ọ bụ enweghị RNA viral, na enweghị ike igosipụta ọrụ nje yana ma nje a na-ebufe ya.Ọ dị mkpa iji gosi ma onye ọrịa nwere ezigbo ule nucleic acid ga-aghọkwa isi iyi nke ọrịa ọzọ.Ọ dị mkpa iji mepụta omenala nje na nlele ụlọ ọgwụ wee mepụta nje "dị ndụ" iji gosi na ọ na-efe efe.

Nchịkọta

Na nchịkọta, SARS-CoV-2 nucleic acid na-anwale ihe ọjọọ na-adịghị mma, nyochaghachi ihe ọma na ọnọdụ ndị ọzọ na-ekwekọghị na ngosipụta ụlọ ọgwụ enweghị ike izere kpamkpam.Na nyocha na nyocha n'ezie, a na-atụ aro ka ikpokọta mgbaàmà ụlọ ọgwụ, nyocha onyonyo (CT) na nnwale Laboratory test (nyocha nucleic acid + nje kpọmkwem antibody test) nsonaazụ maka nyocha zuru oke iji gbochie nchoputa efu na nchọpụta na-ezighi ezi.Ọ bụrụ na achọpụtara na nsonaazụ ule ahụ doro anya na ekwekọghị na ngosipụta ụlọ ọgwụ, a na-atụ aro ka ịme nyocha zuru oke nke njikọ nnwale niile (nchịkọta nlele, mgbasa ozi na njikọ nhazi) iji wepụ nje SARS-CoV-2 n'oge ọrịa, ọrịa na-emegharị ugboro ugboro ma ọ bụ jikọtara ya na nje virus ndị ọzọ na-eku ume, wdg.Ọ bụrụ na ọnọdụ kwere, a na-atụ aro ka ịnakọta ihe nlele ndị ọzọ nwere mmetụta dị ka sputum ma ọ bụ mmiri lavage alveolar maka nyocha ọzọ.

Ngwaahịa ndị emetụtara:

Ngwa SARS-CoV-2 Nchọpụta Nucleic Acid (Usoro nyocha Fluorescent PCR Multiplex)