Nyocha na-esonụ nke nsogbu nwere ike ime naBuccalswab/FTA card DNA extraction is helpful to your experiment. In addition, for other experimental or technical problems in addition to operating instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us at: 028-83360257 or E-mali:Tech@foregene.com.

Akpọchiela kọlụm nchacha

N'ime ngwa a, na ọrụ mmịpụta DNA nke genomic, a na-etinye kọlụm dị ọcha ozugbo na ngwakọta enzymatic lysis sample na-enweghị nzọụkwụ centrifugation, na kọlụm dị ọcha nwere ike igbochi n'ihi enzymization ezughị ezu na viscosity dị elu nke ihe nlele ahụ.

Ihe ndị a nwere ike ịkpata bụ ndị a:

1. Enzymatic mgbaze nke anụ ahụ ezughị ezu.

Nkwanye: Enwere ike ịgbatị oge nhazi ihe nlele nke Foregene Protease nke ọma ma ọ bụ enwere ike iwere ihe dị elu mgbe centrifugation gasịrị na 12,000 rpm (~ 13,400).× g) maka nkeji 5.

2. Iji ihe nlele anụ ahụ ma ọ bụ nnukwu anụ ahụ mee ihe nke ukwuu.

Nkwanye: Ọ kacha mma ka ị ghara ịgafe 1Buccal swab na sample;Ọ bụrụ na ihe nlele ahụ buru ibu, mụbaa dose nke Buffer ST1, Foregene Protease, buffer ST2 otu a.

3. Ihe nlele viscosity dị oke elu.

Nkwanye: Enwere ike were 10mM nke Tris-HCl gbarie ihe nlele nke ọma tupu mmịpụta DNA genomic.

4. Arachala iberibe kaadị ọbara.

Nkwanye: Enwere ike ịgbatị oge centrifugation na-agafe agafe nke nzọụkwụ 6 nke ntụpọ ọbara (FTA Card) mmịpụta mkpụrụ ndụ ihe nketa nke ọma.

Mkpụrụ dị ala ma ọ bụ enweghị DNA

A na-enwekarị ihe dị iche iche nke na-emetụta mkpụrụ DNA genomic, gụnyere ihe atụ sitere, ọnọdụ nchekwa ihe nlele, nkwadebe nlele, nhazi, wdg.

Enweghị ike ịnweta DNA genomic n'oge mmịpụta

Ihe nwere ike ịkpata ya bụ ndị a:

1. Ichekwa ihe nlele ma ọ bụ nchekwa na-ezighi ezi maka ogologo oge na-eduga na mmebi DNA genomic.

Nkwanye: Ọkacha mma swabs ọnụ ka a ga-enyocha ya nke ọma, ọ bụghịkwa mma iji swabs echekwara maka ọrụ mmịpụta DNA genomic;Ihe nlele ntụpọ ọbara kwesịrị ijide n'aka na ogo ahụ tozuru oke yana oge nchekwa ekwesịghị ịdị ogologo.

2. Ojiji nke anụ ahụ dị nta nwere ike ime ka enweghị mmịpụta nke DNA genomic kwekọrọ.

Nkwanye: Sorobuccal ntuziaka nlele swab na ntuziaka ọrụ, wee hichaa ugboro ole o kwere mee ka mkpụrụ ndụ zuru oke wee nwee ike itinye ya na swab ọnụ maka mmịpụta DNA genomic;maka mmịpụta ntụpọ ọbara, enwere ike ịbawanye ebe mbepụ ntụpọ ọbara nke ọma.

3. A na-echekwa Foregene Protease n'ụzọ na-ekwesịghị ekwesị, na-ebute mbelata ọrụ ya ma ọ bụ arụ ọrụ ya.

Nkwanye: Kwado ọnọdụ nchekwa nkenke Foregene Protease ma ọ bụ dochie ya na Foregene Protease ọhụrụ maka mmeghachi omume enzymatic.

4. Nchekwa ezighi ezi nke ngwa ma ọ bụ oge nchekwa dị ogologo, na-ebute ọdịda nke ụfọdụ ihe dị na ngwa ahụ.

Nkwanye: Zụrụ nke ọhụrụBuccal DNA swabnnọpụ iche kit maka usoro ndị yiri ya.

5. Buffer WB anaghị etinye ethanol zuru oke.

Nkwanye: Kwenye na ihe nchekwa WB na-agbakwụnye olu ziri ezi nke ethanol zuru oke.

6. A naghị agbakwunye eluent nke ọma na ihe nkiri silicone.

Nkwanye: Tinye 65Celsius Celuent a na-ekpo ọkụ na-adaba n'etiti akpụkpọ ahụ silicone wee hapụ n'ime ụlọ maka nkeji 5 iji mee ka ọrụ elution dịkwuo elu.

LDNA mkpụrụ ndụ ihe nketa dịpụrụ adịpụ

Ihe ndị a nwere ike ịkpata bụ ndị a:

1. Ichekwa ihe nlele ma ọ bụ nchekwa na-ezighi ezi maka ogologo oge na-eduga na mmebi DNA genomic.

Nkwanye: Ọkachamma swabs ọnụ ka a na-enyocha nke ọhụrụ, na swabs echekwara ekwesịghị iji maka mmịpụta DNA genomic.

2. Ọ bụrụ na ọnụọgụ anụ ahụ dị ntakịrị, ọdịnaya DNA genomic amịpụtara ga-adị ntakịrị.

Nkwanye: Soro ntuziaka nlele ọnụ swab dị na ntuziaka ịwa ahụ, na-ehichapụ ọtụtụ oge ka enwere ike itinye mkpụrụ ndụ zuru oke na swab ọnụ maka mmịpụta DNA genomic.

3. A na-echekwa Foregene Protease n'ụzọ na-ekwesịghị ekwesị, na-ebute mbelata ọrụ ya ma ọ bụ arụ ọrụ ya.

Nkwanye: Kwado ọnọdụ nchekwa nkethe Foregene Protease ma ọ bụ jiri nke ọhụrụ dochie ya Foregene Protease maka mmeghachi omume enzymatic.

4. nsogbu ndị na-egbuke egbuke.

Nkwanye: Jiri Buffer EB maka elution;Ọ bụrụ na ị na-eji ddH₂O ma ọ bụ eluent ndị ọzọ, gosi na pH nke eluate dị n'etiti 7.0-8.5.

5. A naghị agbakwunye eluate nke ọma.

Nkwanye: Tinye eluent tụlee n'etiti akpụkpọ ahụ silicone wee hapụ na ọnụ ụlọ okpomọkụ maka 5 min iji welie elution arụmọrụ.

6. Mmiri elution na-akwakọba ntakịrị.

Nkwanye: Jiri eluent maka elution DNA genomic dị ka achọrọ na ntuziaka, opekata mpeọ dịghị obere karịa 15μl.

Lọ dị ọcha of DNA nke genomicdịpụrụ adịpụ

Ọdịdị DNA dị ala dị ala nwere ike iduga ọdịda ma ọ bụ nsonaazụ na-adịghị mma nke nyocha ndị dị n'okpuru, dị ka: enweghị ike imeghe enzymes, PCR enweghị ike ịnweta mkpụrụ ndụ mkpụrụ ndụ nke mmasị, wdg.

Ihe nwere ike ịkpata ya bụ ndị a:

1. Mmetọ heteroprotein, mmetọ RNA.

Nyocha: A naghị akwọcha kọlụm dị ọcha site na iji Buffer PW;A saghị kọlụm nchacha nke Buffer PW site na iji ọsọ centrifugal ziri ezi.

Nkwanye: Gbaa mbọ hụ na ọ dịghị mmiri ozuzo na supernatant tupu ịgbakwunye ethanol;jide n'aka na ị ga-asacha kọlụm dị ọcha dịka ntuziaka ahụ si dị, na enweghị ike ịhapụ nzọụkwụ a.

2. Mmetọ ion adịghị ọcha.

Nyocha: Ewepụla ma ọ bụ sachaa kọlụm ịsacha ihe nchekwa nke WB naanị otu ugboro, butere mmetọ ionic fọdụrụnụ.

Nkwanye: Jide n'aka na ị ga-asa Buffer WB ugboro 2 ka enyere ya ka iwepu ion fọdụrụ ka o kwere mee.

3. RNA enzyme mmetọ.

Nyocha: Agbakwunyere RNases nke mba ọzọ na nchekwa;Arụ ọrụ ịsacha PW buffer ezighi ezi, butere mfu RNase, na-emetụta ọrụ nnwale RNA nke ala, dị ka ntụgharị in vitro.

Nkwanye: Foregene usoro nucleic acidisolangwa ngwa nwere ike wepu RNA na-enweghị mgbakwunye RNase ọzọ,otu a buccal Swab/FTA Kaadị DNA Isolation Kitmkpa't tinye RNase;jide n'aka na ị ga-agbaso ntuziaka maka kọlụm ịsacha Buffer PW, na enweghị ike ịhapụ usoro a.

4. Ethanol fọdụrụnụ.

Nyocha: Buffer WB emeghị centrifugation tube efu ka ọ sachara kọlụm dị ọcha.

Nkwanye: Mee ezi ọrụ centrifugation tube efu dị ka ntuziaka si dị.

5. Mmetọ adịghị ọcha ndị ọzọ.

Analysis: Echekwara samples ma ọ bụ pụrụ iche sample na-adịghị pretreated.

Nkwanye: Hazie ihe nlele ahụ nke ọma dị ka enyere ya ntụziaka.