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Ụzọ PCR na mmetọ aerosol nke nucleic acid na ụlọ nyocha nyocha nucleic acid dị ka akụkụ abụọ nke otu mkpụrụ ego.Anyị nwere ike họrọ naanị ịnwe ya ma ọ bụ na anyị enweghị, mana anyị enweghị ike ịhọrọ ma anyị chọrọ ya ma ọ bụ na-emefu ya.

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1. Nyochaa ihe na-ewepụ DNA

Iji nweta mwepụta oghere nke nucleic acid aerosol mmetọ, ọ dị mkpa na mbụ enyocha ihe na-ewepụ DNA nke nwere ike wepu acid nucleic na ọnọdụ mmiri mmiri.N'ihi na ọ bụghị ọtụtụ ndị na-ewepụ DNA na-arụ ọrụ n'ezie.Maka usoro nnwale, biko rụtụ aka na: Onye na-ewepụ DNA enweghị ike ịbụ "akụkụ nzuzo" nke ụlọ nyocha!

N'ime nnwale a, 100 mbipụta / μL (CT dị ka 31) nke ASFV plasmid na DNA remover nke ejiri PCR dijitalụ jikọtara ya na mpịakọta nha anya, wee meghachi omume na okpomọkụ ụlọ maka 10min, 20min na 30min n'otu n'otu.Mgbe mmịpụta nucleic acid gasịrị, a na-eme nkwalite qPCR.A na-atụnyere njikwa dị mma jikọtara ya na plasmid na mmiri.Ebe ọ bụ na emechabeghị nnwale ahụ n'otu oge ahụ, enwere ike inwe ọdịiche dị n'etiti nsonaazụ ya, mana ọ naghị emetụta njedebe nke nnwale ahụ.Ka ọ dị ugbu a, enyochala m ngwaahịa mkpochapụ DNA 10 azụmahịa.Naanị ngwaahịa 1, nke 6 na nke 8 nwere ike imebi DNA plasmid nke ọma na steeti mmiri mmiri.Ngwaahịa ndị ọzọ ọ fọrọ nke nta ka ọ bụrụ enweghị mmetụta.

Tebụl 1 Nucleic acid mmetụta mwepụ nke ihe mkpochapụ DNA dị na ahịa

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2. Nnwale mmetụta mwepụ DNA nke ọgwụ nje na-ejikarị eme ihe

1. Nyochaa ọgwụ nje ndị a na-ejikarị eme ihe

Enwere ọtụtụ ụdị ọgwụ nje a na-ejikarị na ụlọ nyocha: aldehydes, phenols, alcohols, quaternary ammonium salts, peroxides, chlorine preparations, acids and bases.Nlere enyochala mmetụta mkpochapụ nke ihe ndị a na-efe efe na ụmụ nje, mana enweghị data nnwale zuru oke na mmetụta mbibi nke acid nucleic.Ụlọ nyocha nyocha nke Nucleic acid chọrọ ọgwụ na-egbu egbu nke na-abụghị nanị na-enwe mmetụta dị mma nke nje nje na microorganisms, kamakwa nwere ike imebi DNA.Nsonaazụ nnwale ahụ gosiri na naanị nkwadebe chlorine abụọ nke ọgwụ nje 84 na trichloroisocyanuric acid na 1 M hydrochloric acid na-emezu ihe achọrọ.Ebe ọ bụ na 1 M hydrochloric acid na-emebi emebi nke ukwuu, a na-atụ aro ka ejiri nkwadebe chlorine mee ihe mkpofu n'ụlọ nyocha nyocha nucleic acid.Agbanyeghị, nkwadebe chlorine na-emebi ọla na enweghị ike iji ya mee ihe maka mkpochapụ ngwa na akụrụngwa.

Isiokwu 2 DNA mwepụ mmetụta nke ụdị disinfectants dị iche iche

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2. Opekempe dị irè ntinye nke nkwadebe chlorine

Ndị na-ekpo ọkụ na-emepụta ihe na-emepụta chlorine nwere mmetụta mmebi DNA siri ike, ma n'ihi nrụrụ ígwè ha na mgbakasị ahụ, a pụrụ iji ha na-ehicha ala, ihe ndị na-abụghị ígwè, ndụmọdụ ntinye, tubes centrifuge na ihe ndị ọzọ ule.

Dị ka "New Coronary Pneumonia Epidemic Ngwọta Nkà na Ụzụ Ntuziaka": Mmetọ (ọbara ndị ọrịa, secretions na vomit) dị chlorine 5g/L-10g/L chlorine-nwere disinfectant;ala, mgbidi na elu ihe na-eji chlorine 1g/L chlorine disinfectant: uwe, akwa na akwa ndị ọzọ na-ebu ụzọ tinye ya na chlorine disinfectant na 0.5g/L dị chlorine maka nkeji 30, wee kpochaa ya dị ka ọ dị na mbụ.

Tebụl 3 Mmetụta mwepụ DNA nke mkpokọta chlorine dị iche iche

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Nsonaazụ ule na-egosi na: mgbe mkpokọta chlorine dị irè karịrị ma ọ bụ nhata 1.2 g/L, ihe na-egbochi chlorine nwere ike imebi plasmid 100 / μL kpamkpam mgbe ejiri ya maka nkeji ise.Mgbe ị na-eme ihe maka nkeji 10, ọgwụ nje 84 nwere mkpokọta chlorine dị irè karịa ma ọ bụ nhata 0.6 g/L nwere ike imebi 100 mbipụta/μL nke plasmids kpamkpam.

3. Nnwale mwepụ ikuku nke nucleic acid aerosol meruru

Kedu ka esi ehicha ikuku ikuku nke nucleic acid aerosols metọru?Ihe ka ọtụtụ n'ime echiche bụ ịmepụta ebe aerosol na-emerụ emerụ wee kpochaa ya, ma ọ bụ hichaa acid nucleic mgbe mmetọ ahụ gasịrị.Maka m, nnwale nke na-enweghị ike ịmegharị ya na nsonaazụ nnwale ahụ enweghị ike nyochaa n'ụzọ ole na ole enweghị isi, yabụ m gbaziri ụzọ ụfọdụ iji kpochapụ ikuku ikuku.Hụ "Gb27948-2020 General Requirement for Air Disinfectants" na "Nkọwa nka nka disinfection"

1. Ihe nyocha

1.1 DNA wepụ: N'ime nnwale a, ahọpụtara DNA abụọ dị na ahịa 6 na 8 na Tebụl 1.

1.2 Nnwale plasmid: N'ịtụle na uru CT nke ọtụtụ mmetọ ụlọ nyocha ga-adị elu karịa 30, ntinye nke ASFV gene plasmid eji oge a bụ ihe dịka 100 mbipụta / μL, na fluorescent quantitative PCR CT dị ihe dịka 31.07.Tinye plasmid na swab owu na-akpụ akpụ wee kpoo ikuku.

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1.3 Ngwa ule: Ngwa disinfection Bilingkehan KVBOX, nke Shenzhen Runlian Environmental Technology Co., Ltd. na-akwado nke ọma, nwere arụmọrụ disinfection ikuku na hydrogen peroxide disinfectant, ma nwee ike igbu spores na microorganisms ndị ọzọ.

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1.4 Oghere ule: windo mbufe mechiri emechi nke ihe dịka 0.1 cubic mita.

2. Usoro ule

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Ọnụ ọgụgụ nke disinfectant etinyere na igwe disinfection bụ 10ml, na ntinye ya bụ 100ml/m3, nke dị elu karịa "GB27948-2020 General Requirements for Air Disinfectants": mgbe ị na-eji ọgwụ nje aerosol, ọnụ ọgụgụ nke disinfectant kwesịrị ịbụ ≤10 ml / m3.

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Mgbe ịgbasa ya, a na-emechi ya maka awa 2, mgbe ahụ, a na-ewepụ swab nke nwere plasmid na TE, a na-edozikwa swab na plasmid na-enweghị fumigation dị ka nchịkwa.Jiri usoro PCR nke fluorescence quantitative maka nchọpụta.

Tebụl 4 Nsonaazụ mwepụ nke ikuku nucleic acid

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3. Nsonaazụ ule

Ụdị DNA abụọ na-ewepụ ihe nwere ike imebi plasmid nke ọma na ọnọdụ mmiri mmiri enweghị mmetụta ọ bụla na nnwale mwepụ ikuku nucleic acid.Ana m atụ anya ịchọta ihe ngwọta nke enwere ike iji wepụ nucleic acid aerosol mmetọ ikuku na ikuku iji dozie nsogbu nke mmetọ ikuku nucleic acid na ụlọ nyocha nyocha nke nucleic acid, ma ọ dịghị ngwaahịa a chọtara maka oge a.Ekwenyere m na n'ọdịnihu, a ga-enwe ndị na-ewepụ ihe nucleic acid na-arụ ọrụ dị elu nke enwere ike iji wepụ ikuku nucleic acid.

4. Hapụ echiche efu ma lekwasị anya na mgbochi

N'agbanyeghị otú ụlọ ọrụ na-etu ọnụ banyere ha nucleic acid mwepụ ngwaahịa, m na-atụ aro na ọtụtụ ndị enyi na-etinye aka na nucleic acid ule na-ahapụ otu ugboro na ihe niile nro na-aghọta eziokwu: ọ bụ nnọọ ike wepụ ikuku emeru site nucleic acid aerosols, ma ọ bụ na-a siri ike mgbochi ụfọdụ na kwa ụbọchị nlekota oru!

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Oge nzipu: Sep-10-2021