Ndị dike PCR bụ ndị na-eme ihe ike
Nkọwa ngwa:
◮Ikwesị ntụkwasị obi dị elu: 6ugboro nke nkịtị Taq enzyme;
◮Ọsọ ọsọ aỌsọ nkwuputa:ọsọ nkwalite bụ 5-10sec/kb, nke bụ 6-12 ugboro nke nkịtị Taq enzyme;
◮Mgbanwe template ọzọ: ọ nwere ike ịkwalite ndebiri DNA dị mgbagwoju anya nke ọma nwere ọdịnaya GC dị elu ma sie ike ịkwalite;
◮Nke ka eluNkwalite arụmọrụ: ọnụ ọgụgụ nke mmụba cycles dị ala karịa nke nkịtị Taq enzyme;
◮Sike nnabata gburugburu ebe obibi: etinyere na 37 Celsius C maka otu izu, na-ejigide ihe karịrị90%ọrụ;
◮Ọ nwere 5'→3' DNA polymerase ọrụ na 5'→ 3' exonuclease ọrụ, na-enweghị 3'→5' exonuclease ọrụ.
Nkọwa Kit
Ngwaahịa a gụnyere ọgbọ ọhụrụ pụrụ iche nke Foregene nke Taq——Foregene Taq Hero, dNTP kachasị mma, MgCl.2, Nchekwa mmeghachi omume, PCR mmeghachi omume enhancer, optimizer na stabilizer.2 × PCR dikeTMSistemụ ngwakọta nwere nnabata dị elu nye ndị na-egbochi PCR karịa sistemu PCR Mix nkịtị, ma nwee ike ịnagide nkwalite PCR dị iche iche dị mgbagwoju anya.Usoro mmeghachi omume pụrụ iche na Taq Hero dị elu na-eme ka mmeghachi omume PCR nwee arụmọrụ nkwalite dị elu, nkọwapụta na uche.
Nkọwapụta
Usoro ngwa
PH-01013: 2 × PCR dikeTMGwakọta 1.7ml × 3
PH-01014: 2 × PCR dikeTMGwakọta 1.7ml × 6
PH-01015: 2 × PCR dikeTMGwakọta 1.7ml × 24
Ngwa ngwa
2× PCR dikeTMNgwakọta gụnyere: 0.1U Taq Hero / μl, 400μM dNTPs, 20mM Tris-HCl (pH8.8), 100mM KCl, 5mM MgCl2, ndị ọzọ stabilizers na enhancers.
Atụmatụ & uru
-Ikwesị ntụkwasị obi dị elu:6ugboro nke nkịtị Taq enzyme;
- Ọsọ ọsọ aỌsọ nkwuputa:ọsọ nkwalite bụ 5-10sec/kb, nke bụ 6-12 ugboro nke nkịtị Taq enzyme;
-Mgbanwe template ọzọ: ọ nwere ike ịkwalite ndebiri DNA dị mgbagwoju anya nke ọma nwere ọdịnaya GC dị elu ma sie ike ịkwalite;
- Nke ka eluNkwalite arụmọrụ: ọnụ ọgụgụ nke mmụba cycles dị ala karịa nke nkịtị Taq enzyme;
-Sike nnabata gburugburu ebe obibi: etinyere na 37 Celsius C maka otu izu, na-ejigide ihe karịrị90%ọrụ;
-Ọ nwere 5'→3' DNA polymerase ọrụ na 5'→ 3' exonuclease ọrụ, na-enweghị 3'→5' exonuclease ọrụ.
Ngwa ngwa
- PCR mmụba nke iberibe DNA
- DNA labeling
- DNA usoro
- PCR gbakwunyere A ọdụ
Nchekwa na ndụ nchekwa
Enwere ike ịchekwa ya ogologo oge na -20 Celsius;maka iji ya eme ihe ugboro ugboro, enwere ike ịchekwa ya na 4 Celsius maka obere oge.
