PCR Hero (Ya na Dye)
Nkọwa ngwa:
◮Ikwesị ntụkwasị obi dị elu: 6 ugboro nke enzyme Taq nkịtị;
◮Ọsọ nkwalite ngwa ngwa
◮Mgbanwe template ọzọ
◮Ịrụ ọrụ nkwalite dị elu
◮Nkwekọrịta gburugburu ebe obibi siri ike: etinye ya na 37 ° C maka otu izu, na-ejigide ihe karịrị 90% ọrụ;
◮Ọ nwere 5'→3' DNA polymerase ọrụ na 5'→ 3' exonuclease ọrụ, na-enweghị 3'→5' exonuclease ọrụ.
Nkọwapụta
5ml, 10ml, 40ml
PCR Hero (Ya na Dye) ngwa mmịpụta gụnyere ọgbọ ọhụrụ pụrụ iche nke Foregene Taq-Foregene Taq Hero, dNTP kachasị mma, MgCl2, ihe nchekwa mmeghachi omume, ihe nkwalite mmeghachi omume PCR, njikarịcha na ihe nkwụsi ike.Usoro 2 × PCR HeroTM Mix (ede) nwere nnabata dị elu nye ndị na-egbochi PCR karịa usoro PCR Mix nkịtị, ma nwee ike ịnagide PCR nkwalite dị iche iche dị mgbagwoju anya.Usoro mmeghachi omume pụrụ iche na Taq Hero dị elu na-eme ka mmeghachi omume PCR nwee arụmọrụ nkwalite dị elu, nkọwapụta na uche.
Ngwa ngwa
| 0.1U Taq dike /μl |
| 400μM dNTPs |
| 20mM Tris-HCl (pH8.8) |
| 100mM KCl |
| 5mM mgCl2 |
| Ndị ọzọ stabilizers na enhancers |
| Ihe nchekwa DNA na-ebu |
Atụmatụ & uru
■Ikwesị ntụkwasị obi dị elu: 6 ugboro nke enzyme Taq nkịtị;
■ Ọsọ nkwuwa okwu ngwa ngwa: ọsọ nkwalite bụ 5-10sec/kb, nke bụ 6-12 ugboro nke enzyme Taq nkịtị;
∎ Mgbanwe nke ndebiri: Ọ nwere ike ịkwalite ndebiri DNA dị mgbagwoju anya dị elu nke ọmaGC ọdịnaya;
■ Ịrụ ọrụ nkwalite dị elu: okirikiri mmụba dị ala karịa enzyme Taq nkịtị;
■ Nnagide gburugburu ebe obibi siri ike: etinye ya na 37°C maka otu izu, na-ejigide ihe karịrị 90% ọrụ;
■ Ọ nwere 5'→3'Ọrụ DNA polymerase na 5'→3'ọrụ exonuclease, na-enweghị 3'→5'ọrụ exonuclease.
■ A na-ejikọta ngwa ahụ na Electrophoresis Loading Buffer, mgbe PCR gwụchara, enwere ike ibunye ihe nlele ahụ ozugbo maka electrophoresis;ọrụ ahụ dị mfe ma dị mfe.
Ngwa ngwa
Mmụba PCR nke iberibe DNA, akara DNA, usoro DNA, PCR tinyere A ọdụ
Nchekwa na Shelf ndụ
Enwere ike ịchekwa ya ogologo oge na -20°C;maka iji ya ugboro ugboro, enwere ike ịchekwa ya na 4°C maka obere oge.
