Ngwa PCR dị ọcha PCR Sistemụ nhichapụ maka DNA dị ọcha
Nkọwa
Ngwa a na-eji kọlụm na usoro nke ụlọ ọrụ anyị mepụtara, nke nwere ike nwetaghachi iberibe DNA dị ọcha nke ọma na sistemụ PCR.Kọlụm na-adị ọcha nwere ike jikọta DNA nke ọma, ma dakọtara ya na usoro reagent pụrụ iche iji wepụ primer dimer ozugbo na-enweghị mgbake gel.
Ngwa ahụ na-eweghachi ọtụtụ iberibe DNA.N'okpuru ọnọdụ nkịtị, enwere ike nwetaghachi iberibe DNA dị mkpụmkpụ dịka 60bp.Enwere ike iji opekempe 30μl nke eluent mee ka mkpokọta DNA nwetagoro dị elu.
Ngwa ahụ dị mfe iji rụọ ọrụ ma nwee usoro ole na ole.Ọ chọrọ naanị centrifugation dị iche iche iji hazie ọtụtụ ihe nlele n'otu oge, enwere ike nweta iberibe DNA enwetara dị ọcha na nkeji iri na ise.
Nkọwapụta
Nkwadebe 50, 100 nkwadebe, 250 nkwadebe
Ngwa ngwa
Ihe nchekwa BD* |
Ihe nchekwa BD-S |
Ihe nchekwa WB1 |
Ihe nchekwa EB |
DNA-naanị kọlụm |
Ntuziaka |
Atụmatụ & uru
- Ogologo mgbake DNA zuru oke: enwere ike nwetaghachi iberibe DNA dị mkpụmkpụ dịka 60bp yana buru ibu dịka 10kb.
-Ike mgbake dị elu: arụmọrụ mgbake na-adịkarị karịa 80%, nke kachasị elu nwere ike iru karịa 95%.
-Ọsọ ọsọ ọsọ: Ọ dị mfe iji rụọ ọrụ, enwere ike mechaa mgbake DNA n'ime nkeji iri na ise na primer dimer nwere ike wepu ozugbo na-enweghị mgbake gel.
-Safety: Ọ dịghị organic reagent mmịpụta a chọrọ.
-Ego dị elu: Iberibe DNA ndị ahụ enwetara dị oke ọcha, nke nwere ike izute nnwale dị iche iche na-esote.
Ngwa ngwa
Ngwa a dabara adaba maka iweghachi iberibe DNA (60bp-10kb) na ngwaahịa PCR.
Usoro ọrụ
Nchekwa na Shelf ndụ
Enwere ike ịchekwa ngwa a maka ọnwa 12 n'okpuru ọnọdụ akọrọ na ụlọ okpomọkụ (15-25 ° C);Ọ bụrụ na achọrọ ịchekwa ya ogologo oge, enwere ike ịchekwa ya na 2-8 Celsius.
Rịba ama: Ọ bụrụ na echekwara ya na obere okpomọkụ, ngwọta na-adịkarị mfe mmiri ozuzo.Tupu ojiji, jide n'aka na ị ga-etinye ihe ngwọta na kit na ụlọ okpomọkụ maka oge.Ọ bụrụ na ọ dị mkpa, kpoo ya na mmiri ịsa mmiri 37 Celsius C maka nkeji 10 iji gbazee mkpọda ahụ, ma gwakọta ya tupu ejiri ya.