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Lnc-RT Heroᵀᴹ I (Na gDNase) (Super Premix maka njikọ mbụ cDNA sitere na lncRNA)
Lnc-RT dikeNwachukwu M (Na gDNase) bu sistemụ ntụgharị ntụgharị nke emepụtara maka lncRNA iji wepu nje DNA na-akpata genomic. 5 × gDNase Mix nwere ike iwepụ ngwa ngwa genome na RNA na 42 Celsius C maka nkeji 2, na-ezere nnyonye anya nke genome na nsonaazụ qPCR.
5 × L-RT dikeNwachukwu Ngwakọta nwere Foregene LncRNA Reverse Transcriptase nke Foregene mepụtara, nke bụ ụdị ọhụrụ nke transcriptase maka lncRNA na usoro ndebiri RNA ndị ọzọ dị ogologo, nke nwere njikọ RNA siri ike karị na arụmọrụ ndekọ ederede dị elu. Usoro kachasị mma na-eme ka ọnụọgụ ntụgharị ntụgharị ngwa ngwa ma nwee ike ịdepụta ndebiri RNA na ọdịnaya GC dị elu na usoro nke abụọ dị mgbagwoju anya Enwere ike imecha njikọ mbụ nke cDNA na 42 Celsius C na 15 nkeji.
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Real Time PCR Easyᵀᴹ-Taqman
Ihe 2X Real PCR dị mfeNwachukwu Mix-Taqman nke Real Time PCR Easy nyereNwachukwu-Taqman kit bụ sistemụ ọhụụ ọhụrụ nke na-eji nyocha ọhụụ akọwapụtara maka mmeghachi mmụba nke PCR Real Time, nke nwere ike melite nkọwa ngwaahịa na mmeghachi omume nke ọma. ROX na-nyere dị ka esịtidem akara dai.
2X Real PCR Dị MfeNwachukwu Mix-Taqman nwere Foregene pụrụ iche na-ekpo ọkụ-mbido Taq DNA Polymerase. Tụnyere nkịtị Taq enzymes, o nwere uru nke elu amplification arụmọrụ, ike kpọmkwem amplification ike na ala mismatch ọnụego. O nwere ike belata mmụba na-enweghị nkọwa ma melite izi ezi nke PCR.
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Real Time PCR Easyᵀᴹ-SYBR Green I kit
Ihe 2X Real PCR dị mfeNwachukwu Mix-SYBR nke Real Time PCR Easy nyereNwachukwu-SYBR Green I kit bụ sistemụ ọhụụ ọhụrụ nke na-eji SYBR Green I maka Real Time PCR amplification mmeghachi omume, nke nwere ike melite oke ngwaahịa na mmeghachi omume mmetụta. ROX na-nyere dị ka esịtidem akara dai. Igwe ọkụ nke ihe a bụ oge 3-5 nke ngwaahịa ndị yiri ya, nke nwere ike ịdị nhịahụ ma jiri nlezianya gosipụta uche nke template template DNA.
2X Real PCR Dị MfeNwachukwu Mix-SYBR nwere ihe dị iche iche mmalite mmalite nke Taq DNA Polymerase nke Foregene. Tụnyere nkịtị Taq enzymes, o nwere uru nke elu amplification arụmọrụ, ike kpọmkwem amplification ike na ala mismatch ọnụego. O nwere ike belata mmụba na-enweghị nkọwa ma melite izi ezi nke PCR.
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PCR Heroᵀᴹ (na dai)
Ihe 2X Real PCR dị mfeNwachukwu Mix-SYBR nke Real Time PCR Easy nyereNwachukwu-SYBR Green I kit bụ sistemụ ọhụụ ọhụrụ nke na-eji SYBR Green I maka Real Time PCR amplification mmeghachi omume, nke nwere ike melite oke ngwaahịa na mmeghachi omume mmetụta. ROX na-nyere dị ka esịtidem akara dai. Igwe ọkụ nke ihe a bụ oge 3-5 nke ngwaahịa ndị yiri ya, nke nwere ike ịdị nhịahụ ma jiri nlezianya gosipụta uche nke template template DNA.
2X Real PCR Dị MfeNwachukwu Mix-SYBR nwere ihe dị iche iche mmalite mmalite nke Taq DNA Polymerase nke Foregene. Tụnyere nkịtị Taq enzymes, o nwere uru nke elu amplification arụmọrụ, ike kpọmkwem amplification ike na ala mismatch ọnụego. O nwere ike belata mmụba na-enweghị nkọwa ma melite izi ezi nke PCR.
