• facebook
  • njikọ
  • youtube
ibe_banner

Mpempe akwụkwọ PCR Direct (na-enweghị Ngwa Nlereanya) Protocol Direct PCR sitere na ihe ọkụkụ.

Nkọwa ngwa:

Jiri akwụkwọ lysate leaf ma ọ bụ akwụkwọ ozugbo mee ihe dị ka ndebiri maka mmụba PCR, na-enweghị mmịpụta DNA dị iche iche, ngwa ngwa na nghọta dị elu.

Ọ dịghị ọcha DNA na-eri oge na ọnụ (oge, ọrụ na ọnụ ahịa echekwara!)

Ihe dị obere

Mfe - enwere ike idobe ihe nlele ahụ na mmeghachi omume PCR ma ọ bụ mmeghachi omume lysis na-enweghị ịkpụ ma ọ bụ egweri

2 X Mix — ibelata njehie nbudata ihe nlele na oge nkwadebe usoro mmeghachi omume

Enwere ike ịmecha nkwadebe ngwa ngwa n'ime nkeji iri, PCR mmeghachi omume nwere ike mechaa na nkeji 50

Enwere ike mezue mmeghachi omume lysis dị elu na efere PCR 96 nke ọma

ike foregene


Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwa

Ngwaahịa a na-eji usoro nchekwa lysis pụrụ iche iji lyse akwụkwọ osisi.Enwere ike iji lysate mee ihe dị ka template na-enweghị ọcha.Mgbe ịgbakwunye primers, PCR Mix nke kit a nwere ike iji mee ka ọ dịkwuo elu.

Nkọwapụta

0 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ngwa ngwa

Akụkụ I

Ihe nchekwa P1

Ihe nchekwa P2

6×Ihe nchekwa DNA na-ebu

Akụkụ II

2× Akwụkwọ PCR MfeTMNgwakọta

Atụmatụ & uru

∎ Ọ dịghị ọcha DNA na-ewe oge yana ọnụ
■ Ihe dị obere
∎ Mfe - enwere ike idobe ihe nlele ahụ na mmeghachi omume PCR ma ọ bụ mmeghachi omume lysis na-egbutughị ma ọ bụ egweri
∎ 2 × Mix, na-ebelata mperi mperi ihe nlele na oge nkwadebe usoro mmeghachi omume
■ Enwere ike ịmechaa nkwadebe ngwa ngwa n'ime nkeji 10, enwere ike mechaa mmeghachi omume PCR n'ime opekata mpe nkeji iri ise.
∎ Enwere ike mechaa mmeghachi omume nnabata dị elu na efere PCR 96 nke ọma

Ngwa ngwa

Ngwa: njirimara gbanwetụrụ na mkpụrụ ndụ ihe nketa, genotyping, wdg.
Ihe atụ: akwukwo osisi
Usoro onunu ogwu: dayameta 2-3mm (usoro ozugbo), dayameta 5-7mm (usoro lysis)
Oke nchọpụta: iberibe ebumnuche ≤1kb

Usoro ọrụ

Akwụkwọ osisi Direct PCR Kit04

Electropherogram

Akwụkwọ osisi Direct PCR Kit05

  • Nke gara aga:
  • Osote:

  • Dee ozi gị ebe a ziga anyị ya