Akwụkwọ osisi Direct PCR gbakwunyere kit-UNG (na-enweghị Ngwa Nlereanya) Protocol Direct PCR sitere na ihe ọkụkụ.
Nkọwa
Dabere na ngwa Plant Leaf Direct PCR Plus, a na-eji dUTP kama dTTP, yana UNG enzyme nke nwere ike imebi template nwere dUTP na-agbakwunye.N'ụzọ dị otú a, a na-achọpụta kpọmkwem na izi ezi nke mmụba, na nsogbu nke mmetọ ngwaahịa PCR nke nwere ike ime n'oge ule mkpụrụ ndụ ihe nketa buru ibu na-egbochi.
Nkọwapụta
50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns
Ngwa ngwa
Akụkụ I | Ihe nchekwa P1 |
Ihe nchekwa P2 | |
6×Ihe nchekwa DNA na-ebu | |
Akụkụ II | 2× Akwụkwọ PCR MfeTMNgwakọta (UNG) |
Atụmatụ & uru
∎ Ọ dịghị ọcha DNA na-ewe oge yana ọnụ
■ Ihe dị obere
∎ Mfe - enwere ike idobe ihe nlele ahụ na mmeghachi omume PCR ma ọ bụ mmeghachi omume lysis na-egbutughị ma ọ bụ egweri
∎ 2 × Mix, na-ebelata mperi mperi ihe nlele na oge nkwadebe usoro mmeghachi omume
■ Enwere ike ịmechaa nkwadebe ngwa ngwa n'ime nkeji 10, enwere ike mechaa mmeghachi omume PCR n'ime opekata mpe nkeji iri ise.
∎ Enwere ike mechaa mmeghachi omume nnabata dị elu na efere PCR 96 nke ọma
Ngwa ngwa
Ngwa: njirimara gbanwetụrụ na mkpụrụ ndụ ihe nketa, genotyping, wdg.
Ihe atụ: akwukwo osisi
Usoro onunu ogwu: dayameta 2-3mm (usoro ozugbo), dayameta 5-7mm (usoro lysis)
Oke nchọpụta: iberibe ebumnuche ≤1kb
Usoro ọrụ
Electropherogram
Nkọwapụta
50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns
Ngwa ngwa
Akụkụ I | Ihe nchekwa P1 |
Ihe nchekwa P2 | |
6×Ihe nchekwa DNA na-ebu | |
Akụkụ II | 2× Akwụkwọ PCR MfeTMNgwakọta (UNG) |
Atụmatụ & uru
∎ Ọ dịghị ọcha DNA na-ewe oge yana ọnụ
■ Ihe dị obere
∎ Mfe - enwere ike idobe ihe nlele ahụ na mmeghachi omume PCR ma ọ bụ mmeghachi omume lysis na-egbutughị ma ọ bụ egweri
∎ 2 × Mix, na-ebelata mperi mperi ihe nlele na oge nkwadebe usoro mmeghachi omume
■ Enwere ike ịmechaa nkwadebe ngwa ngwa n'ime nkeji 10, enwere ike mechaa mmeghachi omume PCR n'ime opekata mpe nkeji iri ise.
∎ Enwere ike mechaa mmeghachi omume nnabata dị elu na efere PCR 96 nke ọma
Ngwa ngwa
Ngwa: njirimara gbanwetụrụ na mkpụrụ ndụ ihe nketa, genotyping, wdg.
Ihe atụ: akwukwo osisi
Usoro onunu ogwu: dayameta 2-3mm (usoro ozugbo), dayameta 5-7mm (usoro lysis)
Oke nchọpụta: iberibe ebumnuche ≤1kb