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Akwụkwọ osisi Direct PCR gbakwunyere kit-UNG (na-enweghị Ngwa Nlereanya) Protocol Direct PCR sitere na ihe ọkụkụ.

Nkọwa ngwa:

Jiri polysaccharide na polyphenol leaf lysate mee ihe ozugbo dị ka ndebiri maka nkwalite PCR, na-enweghị mmịpụta DNA dị iche iche, ngwa ngwa na nghọta dị elu.Agbakwunyela usoro mgbochi mmetọ nke UNG iji gbochie ihe dị mma.

Ọ dịghị ọcha DNA na-eri oge na ọnụ (oge, ọrụ na ọnụ ahịa echekwara!)

Ihe dị obere

Mfe - enwere ike idobe ihe nlele ahụ na mmeghachi omume PCR ma ọ bụ mmeghachi omume lysis na-enweghị ịkpụ ma ọ bụ egweri

2 X Mix — ibelata njehie nbudata ihe nlele na oge nkwadebe usoro mmeghachi omume

Enwere ike ịmecha nkwadebe ngwa ngwa n'ime nkeji iri, PCR mmeghachi omume nwere ike mechaa na nkeji 50

Enwere ike mezue mmeghachi omume lysis dị elu na efere PCR 96 nke ọma

ike foregene


Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwa

Dabere na ngwa Plant Leaf Direct PCR Plus, a na-eji dUTP kama dTTP, yana UNG enzyme nke nwere ike imebi template nwere dUTP na-agbakwunye.N'ụzọ dị otú a, a na-achọpụta kpọmkwem na izi ezi nke mmụba, na nsogbu nke mmetọ ngwaahịa PCR nke nwere ike ime n'oge ule mkpụrụ ndụ ihe nketa buru ibu na-egbochi.

 

Nkọwapụta

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ngwa ngwa

Akụkụ I

Ihe nchekwa P1

Ihe nchekwa P2

6×Ihe nchekwa DNA na-ebu

Akụkụ II

2× Akwụkwọ PCR MfeTMNgwakọta (UNG)

Atụmatụ & uru

∎ Ọ dịghị ọcha DNA na-ewe oge yana ọnụ
■ Ihe dị obere
∎ Mfe - enwere ike idobe ihe nlele ahụ na mmeghachi omume PCR ma ọ bụ mmeghachi omume lysis na-egbutughị ma ọ bụ egweri
∎ 2 × Mix, na-ebelata mperi mperi ihe nlele na oge nkwadebe usoro mmeghachi omume
■ Enwere ike ịmechaa nkwadebe ngwa ngwa n'ime nkeji 10, enwere ike mechaa mmeghachi omume PCR n'ime opekata mpe nkeji iri ise.
∎ Enwere ike mechaa mmeghachi omume nnabata dị elu na efere PCR 96 nke ọma

Ngwa ngwa

Ngwa: njirimara gbanwetụrụ na mkpụrụ ndụ ihe nketa, genotyping, wdg.
Ihe atụ: akwukwo osisi
Usoro onunu ogwu: dayameta 2-3mm (usoro ozugbo), dayameta 5-7mm (usoro lysis)
Oke nchọpụta: iberibe ebumnuche ≤1kb

Usoro ọrụ

Akwụkwọ osisi Direct PCR Plus Kit-UNG4

Electropherogram

Akwụkwọ osisi Direct PCR Plus Kit-UNG5

Nkọwapụta

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ngwa ngwa

Akụkụ I

Ihe nchekwa P1

Ihe nchekwa P2

6×Ihe nchekwa DNA na-ebu

Akụkụ II

2× Akwụkwọ PCR MfeTMNgwakọta (UNG)

Atụmatụ & uru

∎ Ọ dịghị ọcha DNA na-ewe oge yana ọnụ
■ Ihe dị obere
∎ Mfe - enwere ike idobe ihe nlele ahụ na mmeghachi omume PCR ma ọ bụ mmeghachi omume lysis na-egbutughị ma ọ bụ egweri
∎ 2 × Mix, na-ebelata mperi mperi ihe nlele na oge nkwadebe usoro mmeghachi omume
■ Enwere ike ịmechaa nkwadebe ngwa ngwa n'ime nkeji 10, enwere ike mechaa mmeghachi omume PCR n'ime opekata mpe nkeji iri ise.
∎ Enwere ike mechaa mmeghachi omume nnabata dị elu na efere PCR 96 nke ọma

Ngwa ngwa

Ngwa: njirimara gbanwetụrụ na mkpụrụ ndụ ihe nketa, genotyping, wdg.
Ihe atụ: akwukwo osisi
Usoro onunu ogwu: dayameta 2-3mm (usoro ozugbo), dayameta 5-7mm (usoro lysis)
Oke nchọpụta: iberibe ebumnuche ≤1kb

Usoro ọrụ

Akwụkwọ osisi Direct PCR Plus Kit-UNG4

Electropherogram

Akwụkwọ osisi Direct PCR Plus Kit-UNG5

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