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Ngwa Ngwa PCR Kpọmkwem Ngwa Osisi Osisi (na-enweghị Ngwa Nlereanya) Ngwa Ngwa Ngwa Osisi Direct PCR

Nkọwa ngwa:

Ọ dịghị ọcha DNA na-eri oge na ọnụ (oge, ọrụ na ọnụ ahịa echekwara!)

Ihe dị obere

Mfe - enwere ike idobe ihe nlele ahụ na mmeghachi omume PCR ma ọ bụ mmeghachi omume lysis na-enweghị ịkpụ ma ọ bụ egweri

2 X Mix — ibelata njehie nbudata ihe nlele na oge nkwadebe usoro mmeghachi omume

Enwere ike ịmecha nkwadebe ngwa ngwa n'ime nkeji iri, PCR mmeghachi omume nwere ike mechaa na nkeji 50

Enwere ike mezue mmeghachi omume lysis dị elu na efere PCR 96 nke ọma

ike foregene


Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwa

Ngwaahịa a na-eji usoro nchekwa lysis pụrụ iche iji lyse mkpụrụ osisi.Enwere ike iji lysate mee ihe dị ka template na-enweghị ọcha.Mgbe ịgbakwunye primers, PCR Mix nke kit a nwere ike iji mee ka ọ dịkwuo elu.

Nkọwapụta

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ngwa ngwa

Ngwa mkpụrụ osisi Direct PCR Kit I

Akụkụ I

Buffer SP1

Buffer SP2

6 × DNA na-ebufe ihe nchekwa

Akụkụ II

2 × Mkpụrụ PCR dị mfeTMNgwakọta


Mkpụrụ osisi Direct PCR Kit II

Akụkụ I

Buffer SP1

Buffer SP2

6 × DNA na-ebufe ihe nchekwa

Akụkụ II

2 × Mkpụrụ PCR dị mfeTMNgwakọta

Atụmatụ & uru

∎ Ọ dịghị ọcha DNA na-ewe oge yana ọnụ

■ Ihe dị obere

■ Dị Mfe-sample nwere ike doro PCR mmeghachi omume ma ọ bụ lysis mmeghachi omume na-enweghị igbutu ma ọ bụ egweri

■ 2X Ngwakọta-mbenata sample loading njehie na mmeghachi omume usoro nkwadebe oge

■ Ngwa ngwa-Enwere ike ịme nkwadebe template na nkeji 10, enwere ike ịmechaa mmeghachi omume PCR na nkeji 50

■ Ọnụ ahịa dị elu-Enwere ike mechaa mmeghachi omume lysis na efere PCR 96 nke ọma.

Ngwa ngwa

Ngwa: njirimara nke osisi transgenic, genotyping, wdg.

Nlereanya: Osisi osisi ma ọ bụ anụ ahụ nwere polysaccharide dị ala na ọdịnaya polyphenol.

Nlereanya nha: otu mkpụrụ dum, 1-100mg anụ ahụ osisi

Oke nchọpụta: PCR ngwaahịa ≤1kb

Usoro ọrụ

osisi mkpụrụ kpọmkwem PCR

Electropherogram

mkpụrụ osisi kpọmkwem PCR-DIAGRAM

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