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Ngwakọta Mkpokọta ihe ọkụkụ RNA

Nkọwa ngwa:

RNase-efu

Wepu DNA nke ọma site na iji kọlụm DNA- Cleaning

Wepu DNA na-agbakwunyeghị DNAse

Mfe-arụ ọrụ niile na-arụcha na ụlọ okpomọkụ

Ngwa ngwa-enwere ike ịrụcha ọrụ n'ime nkeji iri atọ

Safe — ọ dịghị organic reagent eji

foregene strength


Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwapụta

Nkwadebe 50, 200 nkwadebe

Ngwa ahụ na-eji kọlụm ogho na usoro nke Foregene mepụtara, nke nwere ike wepụta mkpokọta RNA dị ọcha na ịdị elu nke ọma site na anụ ahụ osisi dị iche iche nwere polysaccharides dị ala na ọdịnaya polyphenols.Maka nlele osisi nwere nnukwu polysaccharides ma ọ bụ polyphenols, a na-atụ aro ka iji Plant Total RNA Isolation Plus Kit nweta nsonaazụ mmịpụta RNA ka mma.Ngwa ahụ na-enye kọlụm DNA-Cleaning nke nwere ike wepu DNA genomic ngwa ngwa na lysate nke anụ ahụ.Kọlụm RNA naanị nwere ike jikọta RNA nke ọma.Ngwa ahụ nwere ike ịhazi ọnụ ọgụgụ dị ukwuu nke ihe nlele n'otu oge.

Sistemu niile enweghị RNase, yabụ na RNA emechara adịghị eme ka ọ dị ala.Buffer PRW1 na Buffer PRW2 nwere ike hụ na RNA enwetaghị protein, DNA, ion, na ogige organic emerụghị ya.

Ngwa ngwa

Buffer PSL1, ihe nchekwa PS, ihe nchekwa PSL2

Ihe nchekwa PRW1, ihe nchekwa PRW2

ddH na-efu RNase2O, kọlụm na-ehicha DNA

RNA-naanị kọlụm

Ntuziaka

Atụmatụ & uru

■ Arụ ọrụ na ụlọ okpomọkụ (15-25 ℃) n'ime usoro dum, na-enweghị ice bat na obere okpomọkụ centrifugation.
∎ Ngwa RNase anaghị akwụ ụgwọ zuru oke, ọ nweghị mkpa ichegbu onwe gị maka mmebi RNA.
∎ kọlụm DNA- Cleaning na-ejikọta kpọmkwem na DNA, ka ngwa ahụ wee nwee ike wepụ nje nje DNA na-etinyeghị DNA.
∎ Mkpụrụ RNA dị elu: Kọlụm naanị RNA nwere ike sachaa RNA nke ọma.
∎ Ọsọ ọsọ: dị mfe iji rụọ ọrụ ma nwee ike mechaa n'ime nkeji iri atọ.
■ Nchekwa: ọ dịghị organic reagenti a chọrọ.
■ Ọdịmma dị elu: Iberibe RNA ndị a sachara dị ọcha, enweghị protein na adịghị ọcha ndị ọzọ, ma nwee ike zute ngwa nnwale dị iche iche nke ala.

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Ngwa ngwa

Ọ dabara maka mmịpụta na ịdị ọcha nke mkpokọta RNA site na nlele anụ ahụ dị ọhụrụ ma ọ bụ oyi kpọnwụrụ (karịsịa anụ ahụ ahịhịa ọhụrụ) nwere obere polysaccharide na ọdịnaya polyphenol.

Usoro ọrụ

plant total RNA-simple workflow

Eserese

Plant Total RNA Isolation Kit6

Plant Total RNA Isolation Kit Plus na-ahazi 50mg nke akwụkwọ ọhụrụ nke polysaccharides na polyphenols, na 5% RNA dị ọcha ka ejiri electrophoresis nwalee.
1: banana
2: Ginkgo
3: owu
4: pọmigranet

Nchekwa na ndụ nchekwa

Enwere ike ịchekwa ngwa ahụ maka ọnwa 12 n'ime ụlọ okpomọkụ (15-25 ℃) na ebe akọrọ, yana 2-8 ℃ maka ogologo oge (ọnwa 24).

Enwere ike ịchekwa ihe nchekwa PSL1 na 4 ℃ maka ọnwa 1 mgbe agbakwunyere 2-hydroxy-1-ethanethiol (nhọrọ).


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