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  • RT Easyᵀᴹ II Master Premix for first-strand cDNA synthesis for Real Time PCR

    RT Easyᵀᴹ II Master Premix maka njikọ cDNA mbụ maka PCR ozugbo.

    - Sistemụ ntụgharị ntụgharị nke ọma, ọ na-ewe naanị nkeji iri abụọ iji mezue njikọ nke eriri cDNA mbụ.

    - Sistemụ ntụgharị ntụgharị uche dị elu, ndebiri ọkwa pg nwekwara ike nweta cDNA dị elu.

    - Usoro ntụgharị ntụgharị nwere nkwụsi ike dị elu, okpomọkụ mmeghachi omume nke sistemụ nwere ike ịdị elu dị ka 50 ℃, yana ọ nwere arụmọrụ ntụgharị ntụgharị dị mma.

    - Ọbụna ụdị RNA na-adịghị ọcha (mmanya ruo 60%, nnu guanidine ruo 750mM) nwere ike ịdaba na mmeghachi omume ntụgharị.

    - 2 × RT ma ọ bụ dị mfeTM Ngwakọta agbakwunyela primers reverse transcription (Random Primer, Oligo(dT)18 Ihe mbụ).

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