PCR ozugbo

Kedu ihe bụ PCR Direct

PCR Direct bụ usoro nke mmụba DNA ozugbo site naọbara, anụ ahụ anụ ahụ,oke oke,cell, nti oke,zebra azụ, akwa azụ,osisi epupụta,mkpụrụma ọ bụ ihe nlele anụ ahụ mbụ dị ndụ ndị ọzọ na-enweghị ime ikewapụ DNA na usoro nchacha.Usoro PCR ozugbo nwere ike ibelata oge nnwale, yana ọnụ ahịa genotyping na nnukwu ọrụ.Ọ na-enyekwa nhọrọ ka mma ma ọ bụrụ na ọ na-eche ihe ịma aka ndị dị n'ịmụba obere ihe nlele anya ebe nzọụkwụ nchacha nwere ike iduga n'efu.

Ngwakọta FOREGENE's PCR nwere nguzogide nrụgide siri ike, n'ihi ike nke FOREGENE ike patented Taq enzyme (patent nyere ikike: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)).Enzyme Taq ya na-eji teknụzụ recombination DNA iji jikọta mpaghara nhazi njikọ DNA nke protein nucleic acid nke archaeal na Taq DNA polymerase na-eguzogide okpomọkụ iji wuo DNA ndebiri nwere mmekọrịta dị ukwuu.Taq mmalite na-ekpo ọkụ ka mma nke na-ejigide ọrụ dị iche iche nke DNA polymerase mbụ. Nke a Taq enzyme nwere ike jikọta na DNA DNA ma malite njikọ DNA na ntinye uche dị ala na gburugburu ebe mgbagwoju anya.

For ọmụmaatụ,itdị mfe dị ka ịgbakwunye gịcell,osisi (obere akwukwo, mgbọrọgwụ ma ọ bụ mkpụrụ) ma ọ bụ anụmanụ sample kaihe nchekwa, na-agbakwunye pụrụ ichenna ukwu mix nakpọmkwemprimers na PCR tubes na-agba ọsọ ya site a thermal cycler.Kit arụmọrụ dị mma maka nyocha nlele buru ibu ebe ịchekwa oge dị oke mkpa.

A na-ahọrọ sistemu mmeghachi omume PCR nwere UNG enzyme na dUTP dịka nnwale chọrọ, nke nwere ike zere mmetọ nke ngwaahịa nkwalite PCR kpatara.

asdada (5)
asdada (1)

Uru nkeDPCR dị elu

 

 

PCR ozugbo

Usoro ọdịnala

(RNA dị ọcha+ PCR omenala)

Ihe oriri

oriri anụ ahụ

Mpekarị

Ọtụtụ

Nkwadebe template

Ụdị ihe

Ọ dịghị egweri chọrọ

Ihe atụ egweri

Ọrụ

Ụdị otu tube, 10min

arụ ọrụ mgbagwoju anya, 1h

Flux

1-96

1-24

Mmeghachi omume PCR

usoro mmeghachi omume

Ngwakọta 2 × PCR kachasị mma

Dntp, mgCl2,10 × PCR ihe nchekwa, Taq enzyme,

Angwa

01 Nchọpụta nke microorganisms pathogenic

02 Nchọpụta mkpụrụ ndụ ihe nketa gbanwere, mkpụrụ ndụ ihe nketa

03 Multiplex PCR / SNP nchọpụta / PCR-RFLP

04 Usoro / cloning

Usoro ngwaahịa

 

*Covid Nucleic acid RT-PCR kit, maka ORF1ab, N, E,S, na mutant ndị ọzọ, dị ka Delta variant, wdg.

96Rxns/kit

Uru:

-Mezue ule n'ime ihe dịka otu elekere

- Ọ dịghị mkpa maka mmịpụta RNA na ikewapụ

-Elu nkọwa na uche

Nkọwa ngwa nyocha Covid pịa ebe a

 

 

 

asdada (3)

Usoro

Aha ngwaahịa

Nkọwapụta

Nọmba katalọgụ

Ọnọdụ Nchekwa

Direct RT-qPCR Series

Ọ dị mfeTMNgwa Cell Direct RT-qPCR – SYBR Green I

200T

DRT-01011

Nkeji I 4℃, Nkeji II -20℃

1000T

DRT-01012

asdada (12)

 

Usoro

Aha ngwaahịa

Nkọwapụta

Nọmba katalọgụ

Ọnọdụ Nchekwa

 

 

 

Usoro ọbara Direct PCR

Ọbara SuperDirectTMNgwa PCR (EDTA)

200 × 20μl rxns

TP-04111

-20 ℃

2000 × 20μl rxns

TP-04113

Ọbara SuperDirectTMPCR Kit (EDTA) -UNG

200 × 20μl rxns

TP-04121

-20 ℃

2000 × 20μl rxns

TP-04123

Ọbara SuperDirectTMPCR ngwa
(Heparin)

200 × 20μl rxns

TP-04211

-20 ℃

2000 × 20μl rxns

TP-04213

Ọbara SuperDirectTMPCR Kit (Heparin) -UNG

200 × 20μl rxns

TP-04221

-20 ℃

2000 × 20μl rxns

TP-04223

 

asdada (7)
asdada (4)

 

Seri

Aha ngwaahịa

Nkọwapụta

Nọmba katalọgụ

Ọnọdụ Nchekwa

Animal Direct PCR Series

Ngwa anụ ahụ Direct PCR (65℃ lysis)

200 × 20μl rxns

TP-01111

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-01113

Ngwa anụ ahụ Direct PCR (65℃ lysis) -UNG

200 × 20μl rxns

TP-01121

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-01123

Mouse Tail SuperDirectTMPCR ngwa
(ọnụ ụlọ okpomọkụ lysis)

200 × 20μl rxns

TP-01311

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-01313

Mpempe PCR nke Mouse Tail Direct (65℃ lysis)

200 × 20μl rxns

TP-01331

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-01333

Mpempe PCR nke Mouse Tail Direct (65℃ lysis) - UNG

200 × 20μl rxns

TP-01341

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-01343

Azụ Zebra Direct PCR Kit (65℃ lysis)

200 × 20μl rxns

TP-01411

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-01413

Azụ Zebra Direct PCR Kit (65℃ lysis) - UNG

200 × 20μl rxns

TP-01421

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-01423

asdada (8)
asdada (10)
asdada (9)
asdada (11)

Usoro

Aha ngwaahịa

Nkọwapụta

Nọmba katalọgụ

Ọnọdụ Nchekwa

Osisi Direct PCR Series

Akwụkwọ osisi Direct PCR Kit

200 × 20μl rxns

TP-02111

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-02113

Akwụkwọ osisi Direct PCR Kit -UNG

200 × 20μl rxns

TP-02121

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-02123

Akwụkwọ osisi Direct PCR Plus Kit
(Polysaccharide polyphenol osisi)

200 × 20μl rxns

TP-02131

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-02133

Akwụkwọ osisi Direct PCR Plus Kit -UNG
(Polysaccharide polyphenol osisi)

200 × 20μl rxns

TP-02141

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-02143

Ngwa mkpụrụ osisi Direct PCR Kit I

200 × 20μl rxns

TP-03111

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-03113

Ngwa ihe ọkụkụ Direct PCR I-UNG

200 × 20μl rxns

TP-03121

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-03123

Mkpụrụ osisi Direct PCR Kit II

200 × 20μl rxns

TP-03131

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-03133

Ngwa ihe ọkụkụ Direct PCR II -UNG

200 × 20μl rxns

TP-03141

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-03143

Ngwa mkpụrụ osisi Direct PCR Plus Kit I (Polysaccharide polyphenol osisi)

200 × 20μl rxns

TP-03151

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-03153

Ngwa ihe ọkụkụ Direct PCR Plus I - UNG(Polysaccharide polyphenol osisi)

200 × 20μl rxns

TP-03161

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-03163

Mkpụrụ osisi Direct PCR Plus Kit II

200 × 20μl rxns

TP-03171

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-03173

Mkpụrụ osisi Direct PCR Plus Kit II - UNG(Polysaccharide polyphenol osisi)

200 × 20μl rxns

TP-03181

Nkeji I 4℃, Nkeji II -20℃

2000 × 20μl rxns

TP-03183