RT-PCR bụ teknụzụ na-ejikọta ntụgharị ntụgharị RT nke RNA na polymerase chain amplification (PCR) nke cDNA.Teknụzụ RT-PCR na-enwe mmetụta dị nro ma na-agbanwe agbanwe, enwere ike iji ya chọpụta ọkwa nke okwu mkpụrụ ndụ ihe nketa na mkpụrụ ndụ / anụ ahụ, ọdịnaya nke nje RNA dị na sel, yana cloning kpọmkwem usoro cDNA nke mkpụrụ ndụ ihe nketa.
Uru isi:
Ngwa RT nke Foregene nwere nkwụsi ike siri ike na 50-65 ℃ mmeghachi omume okpomọkụ (65ºC nwere ike mepee ọkwa nke abụọ nke RNA iji mee ka mmeghachi omume ntụgharị na-aga n'ihu nke ọma)
✱ Nkwenye siri ike, nwere ike ịnabata ụfọdụ ethanol na nnu guanidine
| Sub-Usoro | Aha ngwaahịa | Nkọwapụta | Nọmba katalọgụ | Ọnọdụ Nchekwa |
| PCR Mix Series | 5ml | PH-01013 | -20 ℃ | |
| 40ml | PH-01015 | |||
| 5ml | PH-01013-B | -20 ℃ | ||
| 40ml | PH-01015-B | |||
| 2× High-arụmọrụ PCR mmeghachi omume premix usoro | 1 ml | DP-20031 | -20 ℃ | |
| 3 × 1.7 ml | DP-20032 | |||
| 3 × 1.7 ml | DP-20032-B | -20 ℃ | ||
| 50ml | DP-20034-B | |||
| 2× Ngwa ngwa PCR mmeghachi omume premix usoro 2× PCR Mix | 1 ml | DP-20041 | -20 ℃ | |
| 3 × 1.7 ml | DP-20042 | |||
| 500 × 20μl rxns | QP-01012 | -20 ℃ | ||
| 2000 × 20μl rxns | QP-01014 | |||
| 500 × 20μl rxns | QP-01022 | -20 ℃ | ||
| 2000 × 20μl rxns | QP-01024 | |||
| Usoro RT | 25× 20μl Mmeghachi omume | RT-01011 | -20 ℃ | |
| 100 × 20μl mmeghachi omume | RT-01012 | |||
| 200 × 10μl mmeghachi omume | RT-01022 | -20 ℃ | ||
| 800 × 10μl mmeghachi omume | RT-01023 | |||
| 100 × 20μl mmeghachi omume | RT-01032 | -20 ℃ | ||
| Lnc-RT dikeTM M (ya na gDNase) (Super Premix maka njikọ cDNA nke mbụ sitere na lncRNA) | 25 × 20μl mmeghachi omume | RTL-09098 | -20 ℃ | |
| 50 × 20μl mmeghachi omume | RTL-09099 | |||
| Usoro RT-PCR | 100 rxn | RT-02011 | -20 ℃ | |
| 500 rxn | RT-02012 | |||
| 100 rxn | RT-02021 | -20 ℃ | ||
| 500 rxn | RT-02022 | |||
| Usoro RT-qPCR | 100 rxn | RT-02111 | -20 ℃ | |
| 500 rxn | RT-02112 | |||
| 100 rxn | RT-02131 | -20 ℃ | ||
| 500 rxn | RT-02132 | |||
| Ihe nrịbama DNA | 50 nkwadebe | DL-20011 | 4 ℃ | |
| 200 Preps | DL-20012 | |||
| 50 nkwadebe | DL-30011 | 4 ℃ | ||
| 200 Preps | DL-30012 |
