Real Time PCR Easyᵀᴹ-SYBR Green I kit
Nkọwa ngwa:
Ngwakọta dị mfe-2X PCR iji belata njehie nnwale na oge ọrụ
Ihe nchekwa akọwapụtara nke ọma na mmalite Taq enzyme nwere ike igbochi nkwalite na-abụghị nke akọwapụtara yana nguzobe primer dimer.
Mmetụta dị elu-nwere ike ịchọpụta obere ndebiri nke ndebiri
Ọdịiche dị mma—dakọtara na ngwa PCR kacha adị adị
Nkọwa Kit
2 × Real PCR dị mfeTMNgwakọta-SYBR nke Real Time PCR dị mfeTM-SYBR Green I kit bụ usoro premix ọhụrụ nke na-eji SYBR Green I maka mmeghachi omume nkwalite PCR oge, nke nwere ike melite nkọwapụta ngwaahịa na mmeghachi omume mmeghachi omume.A na-enye ROX ka ọ bụrụ ihe eji agba agba n'ime.The fluorescence ike nke kit a bụ 3-5 ugboro nke ngwaahịa ndị yiri ya, nke nwere ike na-enwe mmetụta karịa na kensinammuo na-egosipụta ịta nke lekwasịrị template DNA.
2 × Ezigbo PCR dị mfeTMMix-SYBR nwere mmalite mmalite Taq DNA Polymerase pụrụ iche nke Foregene.N'iji ya na enzymes Taq nkịtị, ọ nwere uru dị na nrụpụta nkwalite dị elu, ikike nkwalite siri ike yana ọnụ ọgụgụ na-adaghị adaba.Ọ nwere ike belata mmụba na-abụghị nke akọwapụtara ma melite izi ezi nke PCR.
Nkọwapụta
| Ezigbo oge PCR Easyᵀᴹ-SYBR Green I Kit | ||||
| Ngwakọta ngwa (usoro 20μl) | QP-01011 | QP-01012 | QP-01013 | QP-01014 |
| 200T | 500T | 1000T | 2000T | |
| 2 × Ezigbo PCR dị mfeTMNgwakọta-SYBR | 1 ml × 2 | 1.7 ml × 3 | 1.7 ml × 6 | 1.7 ml × 12 |
| 50× ROX ntụaka agbaji | 400 μl | 1 ml | 1 ml × 2 | 1 ml × 4 |
| ddH na-efu DNAase2O | 1.7 ml | 1.7 ml × 2 | 10 ml | 20 ml |
| Ntuziaka | 1 | 1 | 1 | 1 |
Atụmatụ & uru
∎ Mfe—2X PCR Ngwakọta iji belata njehie nnwale na oge ọrụ
∎ Specific—nkwadebe nke kachasị na enzyme Taq mmalite na-ekpo ọkụ nwere ike igbochi nkwalite na-abụghị nke akọwapụtara na nhazi ọkwa mbụ.
∎ Mmetụta dị elu-nwere ike ịchọpụta obere ndebiri nke ndebiri
∎ Ọdịiche dị mma—dakọtara na ọtụtụ ngwa PCR ọnụ ọgụgụ dị adị
Ngwa ngwa
qPCR nyocha
Usoro ọrụ
Eserese
Nchekwa na ndụ nchekwa
Ekwesịrị ịchekwa ngwa a n'ebe dịpụrụ adịpụ na ọkụ ma chekwaa ya na -20 ℃.Ọ bụrụ na ejiri ya ugboro ugboro, enwere ike ịchekwa ya na 4 ℃ maka obere oge (ụbọchị iri).
