RT-PCR Easyᵀᴹ II (nzọụkwụ abụọ)
Nkọwa ngwa:
◮Enwere ike ime nyocha ọnụọgụ RNA ozugbo na ngwa ngwa.
◮Ngwa ahụ na-eji ihe ntụgharị ntụgharị pụrụ iche nke Foregene na Foregene HotStar Taq DNA Polymerase jikọtara ya na sistemu mmeghachi omume pụrụ iche iji melite arụmọrụ nkwalite na nkọwapụta mmeghachi omume ahụ nke ọma.
◮ Usoro mmeghachi omume kachasị mma na-eme ka mmeghachi omume ahụ nwee mmetụta nchọpụta dị elu, nkwụsi ike okpomọkụ siri ike, na ndidi ka mma.
Nkọwapụta
RT-PCR dị mfeTM II(Nzọụkwụ abụọ) nwere reagents niile maka ụzọ abụọ RT-PCR, na-ejikọta mmeghachi omume RT na mmeghachi omume PCR n'otu ngwa ahụ, na-enye RT Mix kachasị na PCR Mix.Ngwakọta RT pụrụ iche nwere ike ijikọ cDNA eriri mbụ dị elu dị mfe na nke ọma. 2× PCRMfeTM Ngwakọtagbakwunyere nwere elu-arụmọrụ na kpọmkwem nkwalite.Ngwakọta organic nke abụọ ahụ na-eme ka nchọpụta nke mkpụrụ ndụ ihe mgbaru ọsọ dịkwuo nro.
Ngwa ngwa
| RT-PCRMfeTM II (nzọụkwụ abụọ) | ||
| Ngwa ọdịnaya | RT-02021 | RT-02022 |
| 100T(20 mlusoro) | 500T(20 mlusoro) | |
| 2 × RT MfeTM Ngwakọta | 1ml | 1.7ml ×3 |
| 2× PCRMfeTM Ngwakọtagbakwunyere | 1ml | 1.7ml ×3 |
| Random Primer (50μM) | 200μl | 1ml |
| Oligo(dT)18 nke mbụ (50μM) | 200μl | 1 ml |
| ddH na-efu RNase2O | 1.7 ml | 1.7 ml |
| Akwụkwọ ntuziaka | 1ibe | 1ibe |
Atụmatụ & uru
-Enwere ike ime nyocha ọnụọgụ RNA ozugbo na ngwa ngwa.
-Ngwa ahụ na-eji ihe ntụgharị ntụgharị pụrụ iche nke Foregene na Foregene HotStar Taq DNA Polymerase jikọtara ya na sistemu mmeghachi omume pụrụ iche iji melite arụmọrụ nkwalite na nkọwapụta mmeghachi omume ahụ nke ọma.
-Usoro mmeghachi omume kachasị mma na-eme ka mmeghachi omume ahụ nwee mmetụta nchọpụta dị elu, nkwụsi ike okpomọkụ siri ike, na ndidi ka mma.
Usoro ọrụ
Nchekwa na Shelf ndụ
1.Ọnọdụ njem
Usoro dum nke njem ụgbọ mmiri na-ekpo ọkụ dị ala, iji hụ na ngwa ahụ dị na ọnọdụ nke <4 °C.
2. Ọnọdụ nchekwa
A na-echekwa ngwa ahụ na -20 Celsius.Chekwaa ngwaahịa ahụ na refrjiraeto na-ekpo ọkụ mgbe niile na -20 Celsius C ozugbo nnatachara ya.Ọ bụrụ na ọnọdụ nchekwa kwesịrị ekwesị,tngwaahịa ya agaghị eweda arụmọrụ ọ bụla n'ime oge nkwado afọ 1.
