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RT-PCR Easyᵀᴹ I (Otu Nzọụkwụ)
◮Ngwa otu nzọụkwụ na-enyere aka ịtụgharị ntụgharị na PCR ka eme ya n'otu tube ahụ.Naanị ọ ga-achọ ịgbakwunye RNA template, kpọmkwem PCR primers na RNase-Free ddH2O.
◮Enwere ike ime nyocha ọnụọgụ RNA ozugbo na ngwa ngwa.
◮Ngwa ahụ na-eji ihe ntụgharị ntụgharị pụrụ iche nke Foregene na Foregene HotStar Taq DNA Polymerase jikọtara ya na sistemu mmeghachi omume pụrụ iche iji melite arụmọrụ nkwalite na nkọwapụta mmeghachi omume ahụ nke ọma.
◮Usoro mmeghachi omume kachasị mma na-eme ka mmeghachi omume ahụ nwee mmetụta nchọpụta dị elu, nkwụsi ike okpomọkụ siri ike, na ndidi ka mma.
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