RT-qPCR Easyᵀᴹ (Otu Nzọụkwụ) -SYBR Green I
Nkọwa
RT-qPCR dị mfeTM (Otu Nzọụkwụ) -SYBR Green I na-eji usoro pụrụ iche iji jikọta mmeghachi omume RT na Real Time PCR nke ọma, nke nwere ike mezue nchọpụta ọnụọgụ nke mkpụrụ ndụ ihe nketa ngwa ngwa.Ngwaahịa ahụ nwere nnabata siri ike, nkọwa dị elu na nkwụsi ike.Ngwaahịa a nwere Foregene HotStar Taq DNA Polymerase nke ụlọ ọrụ pụrụ iche, nke nwere uru nke mmụba ngwa ngwa (2Kb/min), nrụpụta nkwalite dị elu, ikike nkwalite akọwapụtara nke ọma, yana ọnụ ọgụgụ na-adaghị adaba ma e jiri ya tụnyere enzymes Taq nkịtị.A na-eji ya ebe a maka Real Time RT-PCR iji belata mmụba na-abụghị nke akọwapụtara yana melite izi ezi nke PCR.
Nkọwapụta
100T (20μl usoro)
500T (20μl usoro)
Ngwa ngwa
RT-qPCR dị mfeTM(Otu Nzọụkwụ) -SYBR Green I | ||
Ọdịnaya ngwa | RT-02111 | RT-02112 |
100T (20μl usoro) | 500T (20μl usoro) | |
2 × RT-qPCR dị mfeTM Ngwakọta-SYBR | 1 ml | 1.7ml × 3 |
50× ROX ntụaka agbaji | 200μl | 1 ml |
ddH na-efu RNase2O | 1.7 ml | 1.7ml × 3 |
Akwụkwọ ntuziaka | 1 ibe | 1 ibe |
Atụmatụ & uru
- Ngwa otu nzọụkwụ na-eme ntụgharị ntụgharị na qPCR mmeghachi omume abụọ n'otu tube, naanị ịkwesịrị ịgbakwunye RNA template, kpọmkwem PCR primers na RNase-Free ddH.2O.
- Ngwa ahụ nwere ike nyochaa ngwa ngwa RNA viral ma ọ bụ trace RNA.
- Ngwa ahụ na-eji ihe ntụgharị ntụgharị pụrụ iche nke Foregene na Foregene HotStar Taq DNA Polymerase jikọtara ya na sistemu mmeghachi omume pụrụ iche iji melite arụmọrụ nkwalite na nkọwapụta nke mmeghachi omume ahụ nke ọma.
- Usoro mmeghachi omume kachasị mma na-eme ka mmeghachi omume nwee mmetụta nchọpụta dị elu, nkwụsi ike okpomọkụ siri ike, na ndidi ka mma.
- RT-qPCR dị mfeTM(Otu Nzọụkwụ) -SYBR Green I kit na-abịa na ROX n'ime ntụ ntụ ntụ, nke enwere ike iji wepụ ihe mgbaàmà na njehie mgbaàmà n'etiti olulu mmiri, nke dị mma maka ndị ahịa iji na ụdị dị iche iche nke akụrụngwa PCR.
Usoro ọrụ
Ọnọdụ nchekwa
1.Ụgbọ njemọnọdụ
Usoro dum nke ụgbọ njem igbe ice dị ala, iji hụ na ngwa ahụ dị na ọnọdụ <4 ℃.
2.Nchekwa ọnọdụ
A na-echekwa ngwa ahụ na -20 ℃.Chekwaa ngwaahịa a na refrjiraeto na-ekpo ọkụ mgbe niile-20 ℃ ozugbo nnata.Ọ bụrụ na ọnọdụ nchekwa kwesịrị ekwesị, ngwaahịaagaghị eweda arụmọrụ ọ bụla n'ime oge nkwado afọ 1.