Ngwa SARS-CoV-2 Nchọpụta Nucleic Acid (Usoro nyocha Fluorescent PCR Multiplex)
Nkọwa ngwa:
Nke a bụ kpọmkwem PCR:Enweghị mkpa maka ngwa mkpochapụ acid nucleic acid na Sistemu, nke emechara 96 n'ime elekere 1 ma ọ bụ karịa.
◮ Ngwa akụrụngwa dị ala yana ojiji nke ukwuu na-agbanwe agbanwe:Naanị sistemụ PCR Real-Time chọrọ.
LoD na mmetụta dị elu:Nchọpụta nchọta nwere ike iru ọkwa kacha ala dị ka 500 nnomi/ml.
Ezubere iji
Ụkpụrụ nke ule
Emebere primer na nyocha nyocha maka ịchọpụta usoro echekwara nke SARS-CoV-2 (ORF1ab gene, N gene na E gene).Ngwa ahụ nwere njikwa dị n'ime nke a na-eji enyocha ogo ihe atụ.Teknụzụ PCR nke adịla adị na-eji mmeghachi omume polymerase chain (PCR) maka mmụba nke usoro ebumnuche akọwapụtara yana nyocha akọwapụtara maka nchọpụta nke RNA agbagoro.A na-akpọ nyocha ndị ahụ na onye nta akụkọ fluorescent na ihe eji esiji eme ihe.
Nkọwa ngwa ngwa
◮Nkọwapụta:48 Rxns/kit, 96 Rxns/kit
◮ Nkọwapụta:96.72%
Mmetụta:97.92%
Ngwa
| Mba. | Akụkụ | Ọnụ ego | Isi ihe | |||
| 48 Rxn | 96 Rxn | |||||
| 1 | Ntọhapụ nke Nucleic acidonye nnọchi anya | 1.5 ml / tube | 1 tube | 1.5 ml / tube | 1 tube | Surfactant |
| 2 | ihe nchekwa RNA | 27 μl / tube | 1 tube | 53 μl / tube | 1 tube | Ihe mgbochi RNase |
| 3 | SARS-CoV-2ngwọta mmeghachi omume | 800 μl / tube | 1 tube | 1600 μl / tube | 1 tube | Ndabere, nyocha, ihe nchekwa mmeghachi omume, dNTP |
| 4 | SARS-CoV-2ngwakọta enzyme | 80 μl / tube | 1 tube | 160 μl / tube | 1 tube | Hot mmalite Taq enzyme,M-MLV enzyme |
| 5 | SARS-CoV-2njikwa mma | 100 μl / tube | 1 tube | 100 μl / tube | 1 tube | plasmid recombinant nwere ebumnucheiberibe, RNA |
| 6 | SARS-CoV-2njikwa na-adịghị mma | 1200 μl / tube | 1 tube | 1200 μl / tube | 1 tube | Ihe nchekwa TE |
Atụmatụ & uru
Otu esi eji
Usoro 1: Direct PCR usoro
Usoro 2: Viral RNA Isolation + PCR
Usoro 1: Direct PCR usoro ijiIhe nnọchi anya mwepụta nlele
Usoro nchọpụta
Ụgbọ njem na Nchekwa Chain oyi
Ejiri ya site na ìhè ma chekwaa na -20 ± 5 ℃;
Ndụ nchekwa
1 afọ
Ozi ịtụ
