Ngwa kewapụrụ RNA nke viral maka ịdị ọcha nke RNA sitere na Plasma, Serum na ihe nlele ndị ọzọ.
Nkọwa ngwa:
Cat.No.RE-02011/02014
Maka ịsachapụ nje virus RNA site na plasma, serum, mmiri ahụ na-enweghị sel, ihe ndị na-ahụ maka ọdịnala.
Wepụ ngwa ngwa ma sachaa nje virus RNA site na nlele dịka plasma, serum, mmiri ahụ na-enweghị sel na ihe ndị na-ahụ maka ọdịbendị cell.
- Ọ dịghị mkpa ichegbu onwe gị maka mmebi RNA.Ngwa niile bụ RNase-efu
-Mfe-arụ ọrụ niile na-agwụ agwụ na ụlọ okpomọkụ
- Ngwa ngwa-arụ ọrụ nwere ike mechaa n'ime nkeji iri abụọ
-Mkpụrụ RNA dị elu: naanị kọlụm RNA na usoro pụrụ iche nwere ike sachaa RNA nke ọma
-Nchekwa — ọ dịghị organic reagent eji
-Ike nhazi nhazi nke ukwuu-ihe ruru 200μl nwere ike ịhazi oge ọ bụla.
Ọdịmma dị elu - RNA emechara dị ọcha nke ukwuu, enweghị protein na adịghị ọcha ndị ọzọ, ọ nwekwara ike zute ngwa nnwale dị iche iche nke ala.
Nkọwa
Ngwa ahụ na-eji kọlụm atụgharị na usoro nke Foregene mepụtara, nke nwere ike wepụta RNA dị ọcha dị elu na nke dị elu site na nlele dịka plasma, serum, mmiri ahụ na-enweghị sel, na ọdịnala sel nke na-enweghị atụ.Ngwa ahụ na-agbakwụnye kpọmkwem Linear Acrylamide, nke nwere ike ijide obere RNA n'ụzọ dị mfe na nlele.RNA-naanị kọlụm nwere ike jikọta RNA nke ọma.Ngwa ahụ nwere ike ịhazi ọnụ ọgụgụ dị ukwuu nke ihe nlele n'otu oge.
Ngwa ahụ niile enweghị RNase, yabụ RNA ahụ emechara agaghị eweda ya ala.Buffer viRW1 na Buffer viRW2 nwere ike hụ na viral nucleic acid enwetara enweghị protein, nuclease ma ọ bụ adịghị ọcha ndị ọzọ, nke enwere ike iji ya mee ihe ozugbo maka nyocha ihe ọmụmụ ihe omimi nke ala.
Nkọwapụta
Nkwadebe 50, 200 Preps
Ngwa ngwa
| Acrylamide linear |
| Ihe nchekwa viRL |
| Buffer viRW1, ihe nchekwa viRW2 |
| ddH na-efu RNase2O |
| RNA-naanị kọlụm |
| Ntuziaka |
Atụmatụ & uru
- Ọ dịghị mkpa ichegbu onwe gị maka mmebi RNA.Ngwa niile bụ RNase-efu
-Mfe-arụ ọrụ niile na-agwụ agwụ na ụlọ okpomọkụ
- Ngwa ngwa-arụ ọrụ nwere ike mechaa n'ime nkeji iri abụọ
-Mkpụrụ RNA dị elu: naanị kọlụm RNA na usoro pụrụ iche nwere ike sachaa RNA nke ọma
-Nchekwa — ọ dịghị organic reagent eji
-Ike nhazi nhazi nke ukwuu-ihe ruru 200μl nwere ike ịhazi oge ọ bụla.
Ọdịmma dị elu - RNA emechara dị ọcha nke ukwuu, enweghị protein na adịghị ọcha ndị ọzọ, ọ nwekwara ike zute ngwa nnwale dị iche iche nke ala.
Ngwa ngwa
Ngwa ngwa:
Ọ dabara maka mmịpụta na ịdị ọcha nke RNA viral n'ụdị dị ka plasma, serum, mmiri anụ ahụ na-enweghị sel na ọdịnala sel.
Usoro ọrụ
Ọnọdụ nchekwa
- Enwere ike ịchekwa ngwa ahụ maka ọnwa 24 na okpomọkụ nke ụlọ (15-25 ℃), ma ọ bụ 2-8 ℃ maka ogologo oge.
-Linear Acrylamide ngwọta nwere ike ịchekwa na ụlọ okpomọkụ maka ụbọchị 7.Mgbe ị nwetachara ngwa ahụ, biko wepụ ihe ngwọta Linear Acrylamide ma chekwaa ya na -20 ℃.
Mgbe ịgbakwunye Linear Acrylamide na Buffer viRL, enwere ike ịchekwa ya na 2-8℃ ruo 48h.Biko jiri ngwọta akwadoro ọhụrụ.
Ntuziaka maka nyocha nsogbu
The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail：Tech@foregene.com。
Enweghị RNA enweghị ike ịwepụta ma ọ bụ mkpụrụ nke nucleic acid dị ala
A na-enwekarị ọtụtụ ihe na-emetụta arụmọrụ mgbake, dị ka: sample ọdịnaya RNA, usoro ọrụ, elution volume, wdg.
Nyocha nke ihe na-akpatakarị:
1.Ice bath ma ọ bụ obere okpomọkụ (4 Celsius C) centrifugation n'oge ọrụ.
Aro: Arụ ọrụ ụlọ okpomọkụ (15-25 Celsius C), ọ dịghị mgbe ịsa ahụ na ice na obere okpomọkụ centrifuge.
2. Nchekwa ihe nlere na-ezighi ezi ma ọ bụ nchekwa nchekwa maka ogologo oge.
Aro: Chekwaa nlele na -80 Celsius ma ọ bụ ifriizi na mmiri mmiri nitrogen, ma zere iji friza eme ihe ugboro ugboro;nwaa iji ihe nlele anakọtara ọhụrụ maka mmịpụta RNA.
3.Ezughị ezu sample lysis
Nkwanye: Biko hụ na agwakọtala ihe nlele na ihe ngwọta na-arụ ọrụ (Linear Acrylamide) ma tinye ya maka nkeji 10 n'ime ụlọ okpomọkụ (15-25 Celsius C).
4.Agbakwunyere eluent na-ezighi ezi
Nkwanye: Gbaa mbọ hụ na agbakwunyere RNase-Free ddH2O n'etiti akpụkpọ ahụ nke kọlụm nchacha.
5.Ezigbo olu nke anhydrous ethanol na Buffer viRW2
Aro: Biko soro ntuziaka a, tinye nha kwesịrị ekwesị nke ethanol anhydrous na Buffer viRW2 wee gwakọta ha nke ọma tupu i jiri ngwa ahụ.
6.Ezigbo sample ojiji.
Aro: 200µl nke nlele kwa 500μl nke ihe nchekwa viRL.Oke sample oke ga-ebute mbelata mmịpụta RNA.
7.Ezigbo elution olu ma ọ bụ ezughị ezu elution.
Aro: Oke eluent nke kọlụm nchacha bụ 30-50μl;Ọ bụrụ na mmetụta elution adịghị afọ ojuju, a na-atụ aro ka ịtinye ddH na-enweghị ọkụ ọkụ nke RNase.2O ma gbatịa oge idowe na ụlọ okpomọkụ, dị ka 5-10min
8.Purification kọlụm nwere ethanol residue mgbe rinsing na Buffer viRW2.
Aro: Ọ bụrụ na ethanol ka dịgidere ka emechara ya na Buffer viRW2 na centrifugation tube efu maka nkeji 2, enwere ike ịhapụ kọlụm dị ọcha na ụlọ okpomọkụ maka 5min ka centrifugation na-enweghị tube ga-ewepụ kpamkpam ethanol fọdụrụnụ.
Mmebi nke ụmụ irighiri ihe RNA dị ọcha
Ọdịmma RNA emechara dị ọcha metụtara ihe ndị dị ka nchekwa nlele, mmetọ RNase, na ọrụ.
Nyocha nke ihe na-akpatakarị:
1. A naghị echekwa ihe ndị a na-anakọta n'oge.
Aro: Ọ bụrụ na ejighị ihe nlele ahụ n'oge nchịkọta, biko chekwaa ya na -80 ℃ ma ọ bụ nitrogen mmiri mmiri ozugbo.Maka mmịpụta nke ụmụ irighiri ihe RNA, gbalịa iji ihe nlele anakọtara ọhụrụ mgbe ọ bụla enwere ike.
2.Achịkọtara samples bụ kefriza na thawing ugboro ugboro.
Aro: Zenarị oyi na agbaze ugboro ugboro (ọ dịghị ihe karịrị otu ugboro) n'oge nchịkọta na nchekwa, ma ọ bụghị ya, mkpụrụ nke nucleic acid ga-ebelata.
E webatara 3.RNase n'ime ụlọ ịsa ahụ ma ọ bụ enweghị uwe, ihe mkpuchi, wdg.
Aro: Mwepụta nke nnwale ụmụ irighiri ihe RNA kacha mma n'ime ụlọ ọrụ RNA dị iche, a na-ehichakwa tebụl nnwale tupu nnwale ahụ.Yiri gloves na masks enwere ike iwepụ n'oge nnwale ahụ ka ịzenarị mmebi RNA nke iwebata RNase kpatara.
4.The reagent na-emerụ site RNase n'oge ojiji.
Aro: Dochie na Viral RNA Isolation Kit ọhụrụ maka nnwale ndị metụtara ya.
5.The RNase mmetọ nke centrifuge tubes, pipette ndụmọdụ, wdg Aro: Jide n'aka na centrifuge tubes, pipette ndụmọdụ, na pipettes niile bụ RNase-free.
Ụmụ irighiri ihe RNA emechara emetụta nnwale ndị dị n'okpuru ala
Ngwakọta RNA ndị a sachara site na kọlụm dị ọcha ga-emetụta nnwale ndị dị n'okpuru ma ọ bụrụ na enwere ion nnu ma ọ bụ protein dị ukwuu, dị ka: ntụgharị ntụgharị, Northern Blot, wdg.
1.There fọdụrụ ion nnu na ụmụ irighiri RNA eluted.
Nkwanye: Gbaa mbọ hụ na agbakwunyere oke nke ethanol anhydrous na Buffer viRW2, wee sachaa kọlụm nke ịdị ọcha ugboro abụọ dịka ọsọ centrifugation ziri ezi na ntuziaka ọrụ; Ọ bụrụ na ion nnu ka fọdụrụ, ị nwere ike itinye Buffer viRW2 na kọlụm nchacha, wee hapụ ya na ụlọ okpomọkụ maka 5min.Mgbe ahụ mee centrifugation iji wepụ mmetọ ion nnu ruo n'ókè kasị ukwuu
2.There fọdụrụ ethanol na eluted RNA ụmụ irighiri
Aro: ozugbo na-akwado na Buffer viRW2 sachara kọlụm nchacha, mee centrifugation tube efu dịka ọsọ centrifugal na ntuziaka ọrụ.Ọ bụrụ na ethanol ka fọdụrụ, enwere ike ịhapụ ya maka nkeji 5 n'ime ụlọ okpomọkụ mgbe centrifugation na-adịghị na tube ga-ewepụ ethanol fọdụrụnụ ruo n'ókè kasị ukwuu.
Akwụkwọ ntuziaka:
Akwụkwọ ntuziaka ngwa mwepu RNA dịpụrụ adịpụ
