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Azụ Zebra Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)

Azụ Zebra Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish) Foto egosipụtara
  • Azụ Zebra Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)
  • Azụ Zebra Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)
  • Azụ Zebra Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)
  • Azụ Zebra Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)

Nkọwa ngwa:

Iji zebrafish lysate mee ihe ozugbo dị ka ndebiri maka nkwalite PCR, enweghị mmịpụta DNA dị iche, ngwa ngwa na nghọta dị elu.Usoro mgbochi mmetọ UNG agbakwunyere iji kpochapụ ihe adịgboroja.

◮Ọ dịghị DNA na-ewe oge na ọnụ ahịa ọcha achọrọ.

◮Ọchịchọ nlele dị ntakịrị, were naanị otu mkpụrụ.

◮Ọ dịghị ọgwụgwọ pụrụ iche dị ka egweri na ịkụpịa ihe a chọrọ, na ọrụ dị mfe.

◮Usoro PCR kachasị mma na-enyere PCR aka inwe nkọwa dị elu yana nnabata siri ike nye ndị na-egbochi mmeghachi omume PCR.

ike foregene


Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwa

Ngwa a na-eji usoro nchekwa lysis pụrụ iche na-ahapụ ngwa ngwa DNA genomic site na zebrafish na anụ ahụ azụ mmiri ndị ọzọ, ọdụ ọdụ ma ọ bụ nsen azụ maka mmeghachi omume PCR, yabụ na ọ dabara adaba maka nyocha mkpụrụ ndụ buru ibu.Usoro nke ịtọhapụ DNA genomic site na nchekwa lysis na-agwụ n'ime nkeji 10-30 na 65 Celsius.Ọ nweghị usoro ndị ọzọ dị ka protein na mwepụ RNA achọrọ, yana DNA achọpụtara ewepụtara enwere ike iji dị ka ndebiri maka mmeghachi omume PCR.

2× PCR EasyTM Mix (UNG) na-eji dUTP kama dTTP na ndabere nke 2 × PCR EasyTM Mix, ma gbakwunye UNG enzyme (Uracil-N-glycosylase) nke nwere ike imebi template nwere dUTP n'otu oge.Tupu mmeghachi omume PCR, a na-eji enzyme UNG mebie ngwaahịa PCR nwere uracil.Enzyme UNG agaghị enwe mmetụta ọ bụla na ndebiri nke na-enweghị uracil, si otú a na-ahụ na nkọwapụta na izi ezi nke nkwalite na igbochi ohere nke nyocha mkpụrụ ndụ ihe nketa buru ibu.Mmetọ nke ngwaahịa PCR mere.

D-Taq DNA polymerase bụ DNA polymerase nke Foregene mepụtara maka mmeghachi omume PCR kpọmkwem.D-Taq DNA polymerase nwere nnabata siri ike maka ọtụtụ ihe mgbochi mmeghachi omume PCR, ma nwee ike ịbawanye ọnụọgụ DNA nke ọma na sistemụ mmeghachi omume dị mgbagwoju anya, yana ọsọ nkwalite nwere ike iru 2Kb / min, nke dabara adaba maka mmeghachi omume PCR Direct.

Nkọwapụta

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ngwa ngwa

Akụkụ I

Ihe nchekwa FP

Protease nke mbụ

Ihe nchekwa 6 × DNA na-ebufe

Akụkụ II

2 × PCR dị mfeTMNgwakọta (UNG)

Ntuziaka

Atụmatụ & uru

■ Ọnweghị ihe na-ewe oge na ọnụ ahịa DNA dị ọcha achọrọ.

■ Ihe nlele achọrọ dị ntakịrị, ọ bụ ezie na enwere ike ịnwale 1mg azụ caudal fin ma ọ bụ akwa azụ 10.

∎ Ọ dịghị ọgwụgwọ pụrụ iche dị ka egweri na ịzọpịa ihe a chọrọ, ma ọrụ ahụ dị mfe.

∎ Sistemụ PCR na-egbochi mmetọ 2 × PCR EasyTM Mix (UNG), nke nwere ike iwepụ mmetọ nke ngwaahịa PCR nke ọma wee hụ na nkọwapụta na izi ezi nke nkwalite.

∎ Sistemu PCR kachasị mma na-eme ka PCR nwee nkọwa dị elu yana nnabata siri ike nye ndị na-egbochi mmeghachi omume PCR.

Ngwa ngwa

Ngwa: azụ zebra na azụ mmiri ndị ọzọ.

DNA ewepụtara na lysis sample: naanị ejiri ya dị ka ndebiri PCR.

Enwere ike iji ngwa ahụ maka ebumnuche ndị a: njirimara transgenosis, genotyping anụmanụ, wdg.

Usoro ọrụ

azụ zebra kpọmkwem PCR

Nchekwa na Shelf ndụ

Ekwesịrị ịchekwa akụkụ I nke ngwa a na 2-8 ℃.

Enwere ike ịchekwa reagent Buffer FP maka ọnwa 12 n'okpuru ọnọdụ akọrọ;Enwere ike ịchekwa ya na 2-8 ℃ maka nchekwa ogologo oge.

v Reagent Foregene Protease nwere usoro pụrụ iche.Iji hụ na ọrụ ya na nkwụsi ike ya, biko chekwaa ya na 4 ℃ maka ọnwa 12.

Enwere ike ịchekwa ihe nchekwa vReagent 6 × DNA na 4℃ ma ọ bụ -20℃ ruo ogologo oge.

Ekwesịrị ịchekwa akụkụ II nke ngwa a na -20 ℃.

vReagent 2×PCR EasyTM Mix(UNG), ọ bụrụ na-eji ya ugboro ugboro, ọ nwekwara ike ịchekwa na 4℃ maka obere oge nchekwa (jiri elu n'ime 10 ụbọchị).

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