Ihe karịrị ọkara nke 2022, bịa hụ ihe akụkọ dị elu nke Foeregene biputere !
01
Ọ bụrụ: 17.694
Biputere ya: Ụlọ Ọgwụ Mgbakwunye, Mahadum Sun Yat-sen
Isi ọdịnaya: Esophageal squamous cell carcinoma (ESCC) bụ isi subụdị nke ọrịa kansa esophageal.Ịmata ebumnuche ọgwụgwọ dị irè dị oke mkpa maka ọgwụgwọ ESCC ka mma.Ụfọdụ ọmụmụ egosila na RNA mismodification nwere ike ịkwalite okwu na ntụgharị mRNA ndị metụtara mkpụrụ ndụ kansa, mana usoro mkpụrụ ndụ ahụ ka edoghị anya.Ọmụmụ ihe a gosiri na tRNA m7G methyltransferase protein mgbagwoju anya metụtara METTL1 na WDR4 abawanyela nke ukwuu na anụ ahụ ESCC ma nyere aka na prognosis na-adịghị mma nke ESCC.Kemgbe ahụ, METTL1 na WDR4 metụtara gene knockout ọmụmụ na ESCC ụdị ụmụ oke ekwenyela na nkwalite ọrụ nke tRNA m7G methyltransferase na-eduga n'ịchịkwa okwu nke protein abụọ a, nke n'aka nke ya na-akwalite mmụba nke mkpụrụ ndụ ESCC.Ọmụmụ ihe a gosipụtara ọrụ oncogenic dị mkpa nke mgbanwe tRNA m7G na-ezighi ezi na ESCC ma na-atụ aro na ịchebe METTL1 na ụzọ mgbama ala ya nwere ike ịbụ ebumnuche ọgwụgwọ maka ọgwụgwọ ESCC.
Ngwaahịa enyere aka:
Mouse Tail Direct PCR Kit Foregene#TP-01333
Njikọ izizi:
https://www.nature.com/articles/s41467-022-29125-7
02
Ọ bụrụ: 14.903
E bipụtara ya: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau
Isi ọdịnaya:
Site na mmepe nke teknụzụ sịntetik, mRNA abụrụla klas ọhụrụ nke ọgwụ ọgwụgwọ.Agbanyeghị, ịnweta nnyefe intracellular nke ọma nke ọgwụ mRNA ka bụ ihe ịma aka n'ihi nghọta nke mRNA na RNases.Ọmụmụ ihe a na-atụpụta atụmatụ nnyefe dabara adaba nke nanostructures jikọtara polyphenol nke nwere ike ịnapụta IL-10 mRNA nke ọma na vivo.Mgbe ejiri nanostructure a na-ebuga ọgwụ mRNA, ọkwa IL-10 na-achịkwa nke ọma, na-egbochi nkwupụta nke ihe ndị na-akpasu iwe, na-akwalite nrụzi mucosal, na-echebe mkpụrụ ndụ epithelial colonic site na apoptosis, na igbochi dextran sodium sulfate na-akpata nnukwu na obere obere na-adịghị ala ala The murine colitis model nwere mmetụta dị ịrịba ama maka ọgwụgwọ ọrịa ọhụrụ.
Ngwaahịa enyere aka:
Ngwakọta RNA dịpụrụ adịpụ nke anụmanụ Foregene# RE-03011
Njikọ izizi:
03
Ọ bụrụ: 12.499
E bipụtara ya: Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University
Isi ọdịnaya:
Nchegbu oxidative nke spam bụ isi ihe na-eduga n'ịmụ nwoke, ya mere ọ bụ nhọrọ dị irè ma dị irè iji guzobe ụdị anụmanụ na-amụghị nwa site n'ịgbanwe usoro antioxidant n'ụzọ ezubere iche.Otú ọ dị, a kọwabeghị usoro antioxidant pụrụ iche nke ụmụ nwoke germ cell.Ọmụmụ ihe a kwadoro na mkpụrụ ndụ LanCL1 bụ mkpụrụ ndụ antioxidant kpọmkwem na mkpụrụ ndụ germ nwoke, na okwu ya na-ebelata ma ọ bụ jikọtara ya na amụghị nwa nwoke.Ụmụ oke oke na-enweghi ụkọ na mkpụrụ ndụ LanCL1 na-egosipụta mmebi sperm oxidative na enweghị ọmụmụ.N'ime usoro sperm dị iche iche nke mkpụrụ ndụ germ, ozugbo ọchịchọ oxidation pụtara, protein protein 1 (SP1) nke ederede ga-edozi okwu nke LanCL1 gene, si otú ahụ na-egbochi usoro oxidation nke spam.Ọmụmụ ihe a na-egosi na ụzọ SP1-LanCL1 na-arụ ọrụ dị mkpa n'ịchịkwa homeostasis testicular na ọmụmụ nwoke na-edozi site na redox itule, ma na-atụ aro na ụmụ oke LanCL1 gbanwere nwere ngwa na-adọrọ adọrọ dị ka ụdị anụmanụ nke enweghị ọmụmụ nwoke.
Ngwaahịa enyere aka:
Mouse Tail DNA Mini Kit Foregene#DE-05211
Njikọ izizi:
http://europepmc.org/article/MED/35469022
Ngwa PCR nke Mouse Tail Direct
◮ Ọ dịghị mkpa DNA na-ewe oge na ọnụ ahịa ọcha.
◮ Ihe nlele achọrọ dị ntakịrị, were naanị otu mkpụrụ.
◮Ọ dịghị ọgwụgwọ pụrụ iche dị ka igweri na nchipịa a chọrọ, na ọrụ dị mfe.
◮ Usoro PCR kachasị mma na-enyere PCR aka inwe nkọwa dị elu yana nnabata siri ike nye ndị na-egbochi mmeghachi omume PCR.
◮ Ọ dịghị mkpa ichegbu onwe gị maka mbibi RNA.Sistemụ a niile enweghị RNase.
◮ Wepụ DNA nke ọma site na iji kọlụm DNA- Cleaning.
◮Wepụ DNA na-agbakwunyeghị DNAse.
◮ Dị mfe - arụ ọrụ niile na-arụcha na ụlọ okpomọkụ.
◮ Ngwa ngwa-enwere ike ịrụcha ọrụ n'ime nkeji iri atọ.
◮ Nchekwa — ọ dịghị organic reagent eji.
◮ Ịdị ọcha dị elu-OD260/280≈1.8-2.1.
◮Enweghị mmetọ RNase:Ogidi DNA-naanị nke ngwa ahụ na-enye na-eme ka o kwe omume iwepụ RNA na DNA genomic na-agbakwunyeghị RNase n'oge nnwale ahụ, na-egbochi ụlọ nyocha ahụ site na RNase exogenous metọọ.
◮Ọsọ ọsọ:Foregene Protease nwere ọrụ dị elu karịa proteases yiri ya, ma na-agbari ihe nlele anụ ahụ ngwa ngwa;arụ ọrụ ahụ dị mfe, yana ọrụ mmịpụta DNA nke genomic nwere ike mechaa n'ime nkeji 20-80.
◮Dị mma:A na-eme centrifugation na okpomọkụ nke ụlọ, ọ dịghịkwa mkpa maka 4 Celsius C obere okpomọkụ centrifugation ma ọ bụ ethanol precipitation nke DNA.
◮Nchekwa:Ọ dịghị organic reagent mmịpụta a chọrọ.
◮Ịdị mma Dị elu:DNA genomic nke ewepụtara nwere nnukwu iberibe, enweghị RNA, enweghị RNase, yana ọdịnaya ion dị oke ala, nke nwere ike izute ihe nyocha dị iche iche chọrọ.
◮Sistemụ Micro-elution:Ọ nwere ike ịbawanye ntinye nke DNA genomic, nke dị mma maka nchọpụta ala ma ọ bụ nnwale.
Oge nzipu: Sep-13-2022
