◮Ikwesị ntụkwasị obi dị elu: 6 ugboro nke enzyme Taq nkịtị;
◮Ọsọ nkwalite ngwa ngwa
◮Mgbanwe template ọzọ
◮Ịrụ ọrụ nkwalite dị elu
◮Nkwekọrịta gburugburu ebe obibi siri ike: etinye ya na 37 ° C maka otu izu, na-ejigide ihe karịrị 90% ọrụ;
◮Ọ nwere 5'→3' DNA polymerase ọrụ na 5'→ 3' exonuclease ọrụ, na-enweghị 3'→5' exonuclease ọrụ.