China Osisi akwukwo Direct PCR Plus kit (enweghị sampling Tools) Protocol Direct PCR si Plant Material Manufacturer and Supplier |Mbido

Ngwa ahịhịa Direct PCR Plus (na-enweghị Ngwa Nlereanya) Protocol Direct PCR sitere na ihe ọkụkụ.

Mpempe akwụkwọ Direct PCR Plus (na-enweghị Ngwa Nlereanya) Protocol Direct PCR sitere na ihe onyonyo egosipụtara.

Nkọwa ngwa:

Jiri polysaccharide na polyphenol leaf lysate mee ihe ozugbo dị ka ndebiri maka nkwalite PCR, na-enweghị mmịpụta DNA dị iche iche, ngwa ngwa na nghọta dị elu.

◮Ọ dịghị ọcha DNA na-eri oge na ọnụ (oge, ọrụ na ọnụ ahịa echekwara!)

◮Ihe dị obere

◮Mfe - enwere ike idobe ihe nlele ahụ na mmeghachi omume PCR ma ọ bụ mmeghachi omume lysis na-enweghị ịkpụ ma ọ bụ egweri

◮2 X Mix — ibelata njehie nbudata ihe nlele na oge nkwadebe usoro mmeghachi omume

◮Enwere ike ịmecha nkwadebe ngwa ngwa n'ime nkeji iri, PCR mmeghachi omume nwere ike mechaa na nkeji 50

◮Enwere ike mezue mmeghachi omume lysis dị elu na efere PCR 96 nke ọma

Zitere anyị email Budata dị ka PDF

Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwa

Ngwaahịa a na-eji usoro nchekwa lysis pụrụ iche iji lyse akwụkwọ osisi.Enwere ike iji lysate mee ihe dị ka template na-enweghị ọcha.Mgbe ịgbakwunye primers, PCR Mix nke kit a nwere ike iji mee ka ọ dịkwuo elu.

Nkọwapụta

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ngwa ngwa

Akụkụ I	Ihe nchekwa PS1
	Ihe nchekwa P2
	6×Ihe nchekwa DNA na-ebu
Akụkụ II	Ihe nchekwa PS2
Akụkụ II	2× Akwụkwọ PCR Mfe^TMNgwakọta

Atụmatụ & uru

∎ Ọ dịghị ọcha DNA na-ewe oge yana ọnụ
■ Ihe dị obere
∎ Mfe - enwere ike idobe ihe nlele ahụ na mmeghachi omume PCR ma ọ bụ mmeghachi omume lysis na-egbutughị ma ọ bụ egweri
∎ 2 × Mix, na-ebelata mperi mperi ihe nlele na oge nkwadebe usoro mmeghachi omume
■ Enwere ike ịmechaa nkwadebe ngwa ngwa n'ime nkeji 10, enwere ike mechaa mmeghachi omume PCR n'ime opekata mpe nkeji iri ise.
∎ Enwere ike mechaa mmeghachi omume nnabata dị elu na efere PCR 96 nke ọma