Mepụta nnwale PCR SOP iji hazie omume nke ndị ọrụ nnwale.
Onye nyocha ahụ na-agbaso usoro arụ ọrụ nke ọma, wee belata mmetọ PCR nke ihe mmadụ nwere ike ịkpata ma ọ bụ gbochie ihe omume mmetọ.Tụkwasị na nke ahụ, onye na-eme nnwale kwesịrị inwe ihe ọmụma na nkà ọkachamara kwekọrọ na ya, gụnyere nkà na ụzụ na-arụ ọrụ metụtara ngwá ọrụ, ịkọwapụta usoro ọrụ dum, ịmụta usoro ọgwụgwọ nke mmetọ na ụzọ njikwa mma ụlọ nyocha, na inwe ike ịkọwapụta nsonaazụ ule n'ụzọ ziri ezi.
Mepụta ụlọ nyocha PCR ọkọlọtọ.
A na-ekewa ụlọ nyocha PCR na mpaghara anọ na ụkpụrụ, ya bụ mpaghara nkwadebe reagent, mpaghara nhazi sample, mpaghara mmụba, na mpaghara nyocha ngwaahịa nkwalite.Ebe abụọ mbụ bụ ebe a na-ebuli elu, na mpaghara abụọ ikpeazụ bụ mpaghara mgbasa ozi.Ekwesịrị ịkewa mpaghara tupu nkwalite na mpaghara mgbasa ozi mgbasa ozi.Nnwale ihe, reagents, ndekọ akwụkwọ, pensụl, ihicha ihe, wdg, nwere ike na-asọpụta site na pre-amplification ebe na post-amplification ebe, ya bụ, site reagent nkwadebe ebe → sample nhazi mpaghara → amplification mpaghara → amplification ngwaahịa analysis ebe, na agaghị aga azụ .Ikuku ikuku dị na ụlọ nyocha kwesịkwara ịgbasa site na ebe a na-amụba ihe na-aga n'ihu na mpaghara post-amplification, ọ bụghịkwa azụ azụ.E gosipụtara ezigbo ụlọ nyocha PCR n'okpuru:
Ọgụgụ A: Ọnọdụ nhazi ụlọ nyocha PCR dị mma nwere nrụgide na-adịghị mma n'ime ụlọ nchekwa
Ọgụgụ B: Ọnọdụ nhazi ụlọ nyocha PCR dị mma nwere nrụgide dị mma n'ime ụlọ nchekwa
Eserese ntọlite ụlọ nyocha PCR enyere na eserese A na onyonyo B kwesịrị ịbụ ọnọdụ ntọlite kacha mma, ụlọ nyocha nwere ọnọdụ nwere ike ịtu aka na ọnọdụ a maka imewe.Maka ụlọ nyocha ndị nkịtị, a na-atụ aro ka enwere ike ikewapụ mpaghara PCR amplification na mpaghara nyocha ngwaahịa, na mmeghe nke mkpuchi kwesịrị ibelata dị ka o kwere mee na mpaghara nkwadebe nlele na mpaghara PCR.Cheta: Ngwaahịa na ihe nnwale na mpaghara nyocha ngwaahịa bụ nke amachibidoro ibuga na mpaghara nkwadebe nlele yana mpaghara nkwalite PCR.
Ọ bụrụ na ụlọ nyocha na-arụ naanị nchọpụta PCR na njirimara, a na-atụ aro ka iji PCR fluorescent quantitative kama PCR.
Enwere ike ịnakọta ma nyochaa nsonaazụ fluorescence quantitative PCR site na akara ngosi fluorescence, yabụ na ọ dịghị mkpa imeghe mkpuchi maka electrophoresis mgbe mmeghachi omume ahụ gasịrị, nke na-ezere mmetọ ngwaahịa PCR nke nhịahụ nke ngwaahịa mmeghachi omume na-etolite ikuku ikuku.Ọ bụrụ na ị na-abawanye ọnụ ọgụgụ nke oghere okpu n'oge ntinye ntinye nke gel electrophoresis, mmetọ aerosol nwere ike ime.A na-atụ aro ịkwalite ngwa nke PCR quantitative wee jiri nwayọọ nwayọọ dochie PCR qualitative.
A na-eji usoro mmetọ ngwaahịa UNG mgbochi PCR maka mmeghachi omume PCR.
Sistemu na-eji dUTP kama dTTP.Mgbe mmeghachi omume PCR gasịrị, ngwaahịa PCR niile (iberibe DNA) jikọtara ya na dUTP;N'ime usoro mmeghachi omume PCR na-esote, UNG enzyme agbakwunyere na sistemụ na-etinye ya na 37 Celsius C maka nkeji 5 tupu PCR, nke nwere ike imebi iberibe DNA niile nwere dUTP, wee mee mmeghachi omume PCR.Nke a nwere ike wepụ kpamkpam mmetọ aerosol nke ngwaahịa PCR kpatara.E gosipụtara mmetụta ahụ na foto dị n'okpuru:
Mara: Maka usoro PCR ozugbo, ị nwere ike họrọ ngwaahịa usoro nke sistemu mmetọ ngwaahịa PCR nke Foregene.Tụọ aro
Maka ụlọ nyocha ndị na-eme nnwale genotyping buru ibu, a na-atụ aro ka ị jiri sistemu mmetọ ngwaahịa UNG mgbochi PCR maka nnwale reagents na mgbakwunye na iwu ụlọ nyocha ezi uche dị na ya.
Ncheta: Iji usoro a enweghị ike iwepụ mmetọ ngwaahịa PCR nke ebutelarị.Ya mere, a ga-eji usoro UNG mee ihe na mmalite nke ule dị mkpa, a ga-ejikwa usoro UNG mee ihe maka mmụba PCR, iji gbochie mmetọ nke ngwaahịa PCR.
A na-atụ aro ka iji usoro PCR-UNG Direct nke Foregene mgbe ị na-eme nnwale buru ibu, dịka:
Akwụkwọ osisi Direct PCR Kit-UNG;
Mpempe akwụkwọ PCR nke mkpụrụ osisi - UNG;
Anụ anụ Direct PCR Kit-UNG;
Kit-UNG Mouse Tail Direct PCR;
Azụ Zebra Direct PCR Kit-UNG.
Usoro ngwa a sitere na Foregenenwere ike ọ bụghị naanị ịme nchọpụta PCR ngwa ngwa na n'ọtụtụ buru ibu, kamakwa igbochi ma jikwaa mmetọ ngwaahịa PCR nke ọma.
Oge nzipu: Mar-19-2021
