Usoro ụlọ SOP

Mepụta nnwale PCR SOP iji hazie omume nke ndị ọrụ nnwale.

Ndị na-eme nnwale ahụ na-agbasosi usoro ọrụ ahụ anya, ma belata mmetọ PCR nke ihe mmadụ nwere ike ịkpata ma ọ bụ gbochie mmetọ mmetọ n'ime ọrụ ahụ.Tụkwasị na nke ahụ, onye na-eme nnwale kwesịrị inwe ihe ọmụma na nkà ọkachamara kwekọrọ na ya, gụnyere nkà na ụzụ na-arụ ọrụ metụtara ngwá ọrụ, ịkọwapụta usoro ọrụ dum, ịmụta usoro ọgwụgwọ nke mmetọ na ụzọ njikwa mma ụlọ nyocha, na inwe ike ịkọwapụta nsonaazụ ule n'ụzọ ziri ezi.

Ụlọ nyocha PCR ọkọlọtọ

A na-ekewa ụlọ nyocha PCR na mpaghara anọ n'ụkpụrụ, ya bụ mpaghara nkwadebe reagent, mpaghara nhazi nlele, mpaghara mmụba, na mpaghara nyocha ngwaahịa nkwalite.Ebe abụọ mbụ bụ ebe a na-ebuli elu, na mpaghara abụọ ikpeazụ bụ mpaghara mgbasa ozi.Ekwesịrị ịkewa mpaghara tupu nkwalite na mpaghara mgbasa ozi mgbasa ozi.Ihe nnwale, reagents, akwụkwọ ndekọ, pensụl, ihe nhicha, wdg nwere ike na-asọpụta naanị site na ebe a na-ebuli elu na mpaghara post-amplification, ya bụ, site na mpaghara nkwadebe reagent, mpaghara nhazi sample, mpaghara mmụba, na mpaghara nyocha ngwaahịa amplification, ma ghara ịgbaba na ntụgharị ntụgharị.Ikuku ikuku dị na ụlọ nyocha ahụ kwesịkwara ịgbanye site na ebe a na-ebuli elu na mpaghara mgbasa ozi, ọ bụghị na ntụgharị ihu.

Belata usoro nnwale

Ọ bụrụ na ụlọ nyocha na-arụ naanị nchọpụta PCR na njirimara, a na-atụ aro ka iji PCR fluorescent quantitative kama PCR.

Enwere ike ịnakọta ma nyochaa nsonaazụ nyocha nke Fluorescence nke PCR site na akara fluorescent, yabụ na ọ dịghị mkpa imeghe mkpuchi maka electrophoresis mgbe mmeghachi omume ahụ gasịrị, nke na-ezere mmetọ nke ngwaahịa PCR nke kpatara ntapu nke ngwaahịa mmeghachi omume na-etolite aerosols.Ọ bụrụ na ị na-abawanye ọnụ ọgụgụ nke oghere okpu n'oge ntinye ntinye nke gel electrophoresis, mmetọ aerosol nwere ike ime.A na-atụ aro ịkwalite ngwa nke PCR quantitative wee jiri nwayọọ nwayọọ dochie PCR qualitative.

Nweta usoro mgbochi mmetọ nke UNG

A na-eji usoro mmetọ ngwaahịa UNG mgbochi PCR maka mmeghachi omume PCR.

Sistemu na-eji dUTP kama dTTP.Mgbe mmeghachi omume PCR gasịrị, ngwaahịa PCR niile (iberibe DNA) jikọtara ya na dUTP;na-esote nke mmeghachi omume PCR, UNG enzyme agbakwunyere na usoro a na-etinye ya na 37 ° C maka nkeji 5 tupu PCR, nke nwere ike ịkọwapụta Degrade niile iberibe DNA nwere dUTP, wee mee mmeghachi omume PCR.Nke a nwere ike wepụ kpamkpam mmetọ aerosol nke ngwaahịa PCR kpatara.E gosipụtara mmetụta ahụ na foto dị n'okpuru:

Mara: Maka usoro PCR ozugbo, ị nwere ike họrọ ngwaahịa usoro nke sistemu mmetọ ngwaahịa PCR nke FJ Biotech.

Tụọ aro

Maka ụlọ nyocha ndị na-eme nnwale genotyping buru ibu, a na-atụ aro ka ị jiri sistemu mmetọ ngwaahịa UNG mgbochi PCR maka nnwale reagents na mgbakwunye na iwu ụlọ nyocha ezi uche dị na ya.

Ncheta: Iji usoro a enweghị ike iwepụ mmetọ ngwaahịa PCR nke ebutelarị.Ya mere, a ga-eji usoro UNG mee ihe na mmalite nke ule dị mkpa, na usoro UNG kwesịrị iji ya mee ihe mgbe niile maka PCR amplification, ka ọ ghara igbochi mmetọ nke ngwaahịa PCR.Echiche ụgha.

A na-atụ aro ka iji sistemu PCR-UNG Direct nke Forge Biotech mgbe ị na-eme nnwale buru ibu, dị ka: Plant Leaf Direct PCR Kit-UNG;

Mpempe akwụkwọ PCR nke mkpụrụ osisi - UNG;

Anụ anụ Direct PCR Kit-UNG;

Kit-UNG Mouse Tail Direct PCR;

Azụ Zebra Direct PCR Kit-UNG.

Usoro ngwa a sitere na Foregene nwere ike ọ bụghị naanị ịme nchọpụta PCR ngwa ngwa na n'ọtụtụ buru ibu, kamakwa igbochi ma chịkwaa mmetọ ngwaahịa PCR nke ọma.