Ego ole ka ị maara

Ihe gbasara Nucleic acid mmịpụta

Mmalite na njedebe nke nucleic acid sachara

Ihe niile siri ike na mmalite, nucleic acid dị ọcha bụ nke kachasị

Mmalite nke nnwale molekụla, maka nnwale ndị na-esote

Ihe ịga nke ọma ma ọ bụ ọdịda nwere mmetụta dị oke mkpa.

Trace/ultra-trace UV spectrophotometer na-amasị ọtụtụ ndị nchọpụta sayensị n'ihi na ọ nwere ike ịchọpụta ntinye nucleic acid na protein na ion nnu dị mfe, ngwa ngwa na akụ na ụba.

Dị ka anyị niile maara, A260 pụtara nucleic acid absorbance OD uru, A280 pụtara protein itinye uche absorbance OD uru, A230 pụtara nnu ion itinye uche absorbance OD uru, ya mere A260 nwere ike tọghata nucleic acid ịta, A260/280 pụtara protein fọdụrụnụ, A260/230 pụtara nnu ion fọdụrụnụ, ma nke a bụ nnọọ a ọnụ ọgụgụ nke nchoputa dabeere a iwu na-achọpụta na a ọnụ ọgụgụ na-adabere a iwu na-achọpụta na a ọnụ ọgụgụ nke na-eme nnyocha.omenala” ikwere na ọ nwere ike ịkọwa ịdị ọcha nke DNA na RNAn'ókè ụfọdụ.Ọ bụghị naanị ọkọlọtọ maka njirimara nke mkpụrụ nucleic acid mkpụrụ na ịdị ọcha, a na-eji ya naanị dị ka ntụaka, ọ bụghịkwa "ọkọlọtọ ọla edo".

Akwụkwọ ntuziaka Thermo Fisher ND-2000 na-emesi ntụkwasị obi nke nsonaazụ ule ahụ ike

Ihe kpatara ya bụ na nsonaazụ enwetara site na iji micro / ultramicro UV spectrophotometer na-egosipụta naanị mkpụrụ na ịdị ọcha nke nucleic acid sitere n'akụkụ, na enwere ọtụtụ ihe na-emetụta (ụzọ anya, olu nlele, ntinye ihe nlele, uru pH, ion ndị ọzọ na-emetụta nha nnabata, wdg) , uru OD tụrụ atụ abụghị eziokwu.N'otu oge ahụ, n'ihi enweghị nkọwa nke ihe mkpebi nke uru nnabata, DNA nwere otu eriri, DNA nwere eriri abụọ, RNA, dNTPs, na ụfọdụ protein niile nwere ọnụ ọgụgụ kachasị elu na 260nm wavelength, na ntinye nke A260 kpebisiri ike abụghị ezigbo DNA ma ọ bụ RNA.A pụghị ikpebi itinye uche, na iguzosi ike n'ezi ihe na mmebi ya.

Ya mere, olee otú ikpe àgwà nke anyị ọcha nucleic acid?Na mgbakwunye na iji micro / ultramicro UV spectrophotometer maka nchọpụta, a na-achọkwa ụzọ ndị dị ka agarose gel electrophoresis iji gosipụta ịdị ọcha na iguzosi ike n'ezi ihe nke nucleic acid, na igosi ntinye uche ruo n'ókè ụfọdụ.N'ụzọ dị otú a, mkpụrụ nucleic acid na ịdị ọcha nke enwetara site na nchọpụta nchọpụta nke ụzọ dị iche iche bụ ihe a pụrụ ịtụkwasị obi.

Ntụnyere chaatị nnwale

A na-ewepụta DNA genomic nke mkpụrụ ndụ H1299 site na iji ngwa DNA Extraction nke ụlọ ọrụ A, a hụkwara mmetọ adịghị ọcha dị egwu, na-ebute ụkpụrụ OD dị elu.

(Uru nha nha OD na electropherogram kwekọrọ)

Ndekọ nyocha

Enwere “ọkọlọtọ ọla edo” maka mkpụrụ nucleic acid na nnwale ịdị mma?

Dị ka anyị maara, ọ dịghị ụzọ dị mfe, ngwa ngwa na ọnụ ala.Ma ọ bụ spectrophotometer, gel electrophoresis, ma ọ bụ uka spectrometry, ha niile na-egosipụta ntinye na ịdị ọcha nke nucleic acid na ngwọta site na akụkụ dị iche iche, ọ dịkwa mkpa ka a na-ekpe ha ikpe site n'ịtụ aka na ibe ha.

Ndekọ nyocha kacha mma bụ mgbe niilengwa nnwale na-esochi.Ọ naghị emetụta arụmọrụ nke ntụgharị ntụgharị na nkwalite PCR.Inweta ngwaahịa nkwalite a pụrụ ịdabere na ya na ụkpụrụ Ct, na ịnweta usoro zuru oke site n'ịhazi usoro bụ mmịpụta acid nucleic na-aga nke ọma.

N'ịchịkọta, echiche nke oke-naanị tiori kwesịrị ịma aka site n'echiche nke "iji ezigbo downstream experimental results dị ka ezi mmịpụta parameters"!

Ngwa ihe mmịpụta DNA nke Foregene akwadoro iji nweta ịdị ọcha DNA/RNA dị elu:

https://www.foreivd.com/reagent/dna-isolation-series/

https://www.foreivd.com/reagent/rna-isolation-series/