Okwu mmalite:

Ụmụ irighiri ihe RNA nwere ike na-emekọrịta ihe nke ukwuu na ụmụ irighiri ihe ndị ọzọ dị ndụ, dị ka protein, DNA na RNA, wee si otú a dị na sel na ihe dị ndụ n'ụdị ogige macromolecular.N'ime ha, ogige RNA-protein bụ ịdị adị na ọrụ nke RNA Otu n'ime ụdị dị mkpa nke.RNA ịdọrọ ala bụ usoro kpochapụwo maka nyocha na ịchọpụta ụmụ irighiri ihe RNA, ọkachasị protein mmekọrịta nke mkpụrụ ndụ lncRNA, ọtụtụ ndị nyocha na akwụkwọ akụkọ agụmakwụkwọ mba ụwa ghọtara.Taa FORREGENE ga-eso gị kerịta ụkpụrụ nnwale na usoro nka nke RNA wetuo ala, ikekwe ị nwere ike iji ya.

1. Ụkpụrụ nnwale nke RNA na-akwatu teknụzụ:

Ntugharị na vitro na njikọ nke biotin-labeled lekwasịrị anya lncRNA ma ọ bụ lncRNA iberibe (gụnyere iberibe RNA ejiri dị ka njikwa dị mma na nke na-adịghị mma), na itinye ya na mpụta mkpụrụ ndụ;jiri avidin-anchored magnetik beads iji nakọta RNA-protein ma ọ bụ ogige RNA-RNA, Mgbe ịsachara nke ọma, mgbagwoju anya na-apụ n'anya.Ọ bụrụ n'ịchọpụta molecule lekwasịrị anya protein nke na-emekọ ihe na lncRNA, mee protein electrophoresis na ngwaahịa RNA-dọrọ ala, chọpụta protein ezubere iche site na ncha ọlaọcha, wee bepụ gel maka spectrometry uka;na-emekwa ozugbo nyocha nke ọdịda ọdịda anyanwụ na ngwaahịa agbadata;Ọ bụrụ na achọpụtara ya Maka ụmụ irighiri ihe RNA nke na-emekọ ihe na lncRNA, ngwaahịa RNA-wedata na-edobe mmịpụta RNA yana nchọpụta qRT-PCR akọwapụtara.

2. RNA wetuo usoro nnwale teknụzụ:

3. Ọrụ nka maka RNA wetuo FOREGENE:

Omenala cell

Nkwadebe nke mkpokọta cell/nuclear/plasma wepụ protein

RNA wetuo nnwale

SDS-PAGE electrophoresis, ọla ọcha

Mass spectrometry na nnwale WB

Akụkọ nnwale

4. ikpe nnwale

Onu ogugu a na-egosi nchoputa FJ Bio-Bio's RNA wetuo ya na nchọpụta SDS-PAGE electrophoresis, nke akụ na-acha uhie uhie na-aka akara ihe dị iche:

Otu nyocha nke Prọfesọ Song Xu nke Mahadum Sichuan achịkọtala afọ 15 nke mkpokọta ogologo oge na ngalaba nyocha lncRNA.Ndị otu nyocha Foregene Bio na Prọfesọ Song Xu kwadoro ịmalite teknụzụ RNA na-agbadata nke lncRNA, nke ga-enye gị nsonaazụ na-eju afọ maka nnwale gị.