Enwere isi ihe egwu abụọ dị na ụlọ nyocha PCR: ihe egwu biosafety na ihe egwu mmetọ nucleic acid.Nke mbụ na-emerụ ndị mmadụ na gburugburu ebe obibi, na nke ikpeazụ na-emetụta nsonaazụ nke ule PCR.Edemede a bụ maka isi ihe nleba anya ihe egwu ụlọ nyocha PCR yana ọkwa ihe egwu dabara adaba ewetara gị.

01 Nkewa nke ụlọ nyocha PCR

1. Ụlọ nyocha nyocha nke mkpụrụ ndụ ihe nketa

Dị ka ihe ndị a chọrọ nke edemede 1.1 nke Basic Setting Standards for Clinical Gene Amplification Testing Laboratories, PCR laboratories n'ozuzu mejupụtara anọ ebe: reagent nchekwa na nkwadebe ebe, ụdị nkwadebe ebe, amplification mpaghara, na amplification ngwaahịa analysis mpaghara.Ọ bụrụ na ejiri usoro PCR fluorescent ozugbo, enwere ike jikọta mpaghara mmụba na mpaghara nyocha n'otu mpaghara;Ọ bụrụ na ejiri PCR nyocha zuru oke na-akpaghị aka, enwere ike jikọta mpaghara nkwadebe ihe atụ, mpaghara mmụba na mpaghara nyocha n'otu mpaghara.

"Akwụkwọ ọrụ maka New Coronavirus Nucleic Acid Testing in Medical Institutions (Trial Version 2)" kwuru na n'ụkpụrụ, ụlọ nyocha na-eme nnwale coronavirus ọhụrụ kwesịrị ịtọlite ​​​​akụkụ ndị a: ebe nchekwa reagent na ebe nkwadebe, mpaghara nkwadebe ihe atụ, mmụba na mpaghara nyocha ngwaahịa.Ebe atọ ndị a kwesịrị ịdị na-adabere kpamkpam na ibe ha na oghere anụ ahụ, ọ nweghịkwa ike inwe nkwurịta okwu na ikuku.

2. Ime ụlọ nkwadebe ihe atụ

Ọ bụ ezie na enwere ike ịkwadebe ihe nlele naanị na mpaghara nkwadebe ihe atụ, a ka na-achọ ụlọ nkwadebe pụrụ iche mgbe ị na-eme ihe nlele dị mgbagwoju anya na ọnụ ọgụgụ dị ukwuu.Ime ụlọ nkwadebe ihe nlele nwere nnukwu ihe egwu nke nchekwa ndu na mmetọ nucleic acid.

3. Ime ụlọ ọgwụgwọ mkpofu

Ọgwụgwọ mkpofu na-ezighi ezi ga-ebutekwa nnukwu ihe egwu nke biosafety na mmetọ acid nucleic na ụlọ nyocha.Ya mere, ọ dị mkpa ka a na-enyocha ụlọ ebe a na-ekpofu ahịhịa mgbe niile.

02 Isi ihe nleba anya ihe egwu na ụlọ nyocha PCR

A na-ekewa ụlọ nyocha dị iche iche n'ime ụlọ nkwadebe sample, ebe nchekwa reagent na ebe nkwadebe, mpaghara nkwadebe ihe atụ, mmụba na mpaghara nyocha ngwaahịa, na ime ụlọ ọgwụgwọ mkpofu.

Dị ka ụdị saịtị nlele ahụ si dị, a na-ekewa ya na elu, ngwá ọrụ, ihe nlele, nlekota gburugburu ebe obibi na pipette.

Ọkwa dị ize ndụ sitere na ala ruo elu site na otu kpakpando★ ruo kpakpando atọ ★ ★ ★.

1. Ime ụlọ nkwadebe ihe atụ:

A na-eji ya maka ndebanye aha, nkwadebe na nkwụsị nke ihe nlele, na ihe ize ndụ nchekwa ndụ bụ nke kachasị elu.N'ihi na a naghị ewepụta ihe nlele ahụ ma gbasaa, ma e wezụga pipettes ndị na-abanyekarị na nlele ahụ, ihe ize ndụ nke mmetọ nucleic acid n'akụkụ ndị ọzọ dị ntakịrị.

1-4 Nlele na ebe nleba anya

5-8 Nlele na ebe nleba anya

9-12 nlekota ihe nlele

1. Reagent nchekwa na ebe nkwadebe:

A na-eji ya maka nkwadebe nke reagents nchekwa, nkesa nke reagents na nkwadebe nke ngwakọta mmeghachi omume amplification, yana nchekwa na nkwadebe nke ihe oriri dị ka tubes centrifuge na ndụmọdụ pipette.Enweghị kọntaktị kpọmkwem na nlele na enweghị ezigbo nucleic acid na mpaghara a, yabụ ihe ize ndụ biosafety na ihe ize ndụ mmetọ nucleic acid dị ala.

13-16 Nlele na ebe nleba anya

17-22 Nlele na nlekota
3. Isi ihe nkwadebe ihe atụ

A na-eji ya meghee gbọmgbọm mbufe, mebie ihe nrịbama (mgbe ọ dị), wepụ nucleic acid ma tinye ya na tube mmeghachi omume amplification, wdg. Ebe a nwere ike ịgụnye nhazi na mmeghe nke ihe atụ, ihe ize ndụ nchekwa ndụ dị elu, a na-emepụta nucleic acid mmịpụta, na ihe ize ndụ nke mmetọ nucleic acid dị elu.

29 Nlele na ebe nleba anya

4. Mgbakwunye na mpaghara nyocha ngwaahịa:

A na-eji ya maka mmụba nucleic acid.Mpaghara a anaghị agụnye nhazi nlele, yana ihe ize ndụ nchekwa ndụ dị ala.Nkwalite acid nucleic bụ tumadi na mpaghara a, na ihe ize ndụ nke mmetọ nucleic acid bụ nke kachasị elu.

38 Nlele na ebe nleba anya
5. Ime ụlọ ọgwụgwọ ihe mkpofu:

Eji maka nhazi nrụgide dị elu nke ihe atụ.Ihe ize ndụ nchekwa ndụ dị na nhazi nke ihe nlele na mpaghara a dị oke elu.A na-atụ aro ngwaahịa nkwalite nucleic acid ka a na-emeso ya dị ka ihe mkpofu ọgwụ.A naghị atụ aro nrụgide dị elu, ihe ize ndụ nke mmetọ nucleic acid dịkwa ntakịrị.

43-44 Nlele na ebe nleba anya

03 Mejuputa

Oge a anyị depụtara 44 nlekota ihe.A na-eme atụmatụ na ọtụtụ ndị mmadụ ga-ajụ, ọ dị ha mkpa ime ọtụtụ isi ihe?Ee, mee ya niile!M na-atụ aro ka ị buru ụzọ mee nyocha ihe ize ndụ nke ụlọ nyocha nke onwe gị, nke a ga-eme dịka ihe ize ndụ si dị site na elu ruo ala, ị nwekwara ike nyochaa otu ụdị ihe atụ ọnụ, ma ọ bụ ị nwere ike ịmepụta atụmatụ nlele maka nlekota mgbe niile.Na nkenke, ụlọ nyocha ọ bụla nwere ike ịme atụmatụ mmejuputa ya dabere na ọnọdụ nke ya.Ihe ize ndụ kachasị maka ụlọ nyocha nyocha bụ ileghara ihe egwu anya.