Iji bụrụ nsonaazụ nke ọkachamara na nrụzi nke anyị, ụlọ ọrụ anyị enwetala aha ọma n'etiti ndị ahịa niile gburugburu ụwa maka 2019 China New Design China 32 Well Plate Magnetic Bead Method Rna Nucleic Acid Extraction Purification Isolation Kit maka Extractor na FDA kwadoro, ebumnuche nke ụlọ ọrụ anyị bụ mgbe niile inye onyinye kacha mma na ọnụahịa kacha mma.Anyị na-achọ n'ihu ime ụlọ ọrụ gị!
Iji bụrụ n'ihi nke anyị ọpụrụiche na nrụzi nsụhọ, anyị corporation emeriwo ihe magburu onwe aha n'etiti ndị ahịa niile gburugburu ụwa maka.China Viral Nucleic Acid iche, Ngwọta mmịpụta DNA Rna, Anyị na-na-anọgidesi ike na azụmahịa kacha mkpa “Quality First, Ịsọpụrụ nkwekọrịta na guzo site aha ọma, na-enye ndị ahịa na-eju afọ ngwaahịa na ọrụ.” A na-anabata ndị enyi ma ndị nọ n’ụlọ ma ná mba ọzọ ka anyị na ha na-enwe mmekọrịta achụmnta ego ebighị ebi.

Nkọwa ngwa ngwa

Nkwadebe 50, 200 Preps

Ngwa a na-eji kọlụm spine na usoro nke ụlọ ọrụ anyị mepụtara, nke nwere ike wepụ ịdị ọcha dị elu na nke dị elu RNA site na anụ ahụ dị iche iche nke anụmanụ na-arụ ọrụ dị elu. Ọ na-enye DNA-Cleaning Column na-arụ ọrụ nke ọma, nke nwere ike ikewapụ na adsorb genomic DNA site na supernatant na anụ ahụ lysate, dị mfe na-azọpụta oge;Kọlụm naanị RNA nwere ike jikọta RNA nke ọma ma nwee ike hazie ya n'otu oge site na iji usoro pụrụ iche Ọtụtụ nlele.

Usoro dum bụ RNase-Free, nke mere na RNA amịpụtara adịghị emebi emebi;Buffer RW1, Buffer RW2 sistemu ịsacha ihe, nke mere na RNA enwetara enweghị protein, DNA, ion, na mmetọ ngwakọta organic.

Ngwa ngwa:

Atụmatụ & uru

■ Ọ dịghị mkpa ichegbu onwe gị maka mbibi RNA;Sistemụ niile bụ RNase-Free
∎ Wepu DNA nke ọma site na iji kọlụm na-ehicha DNA
■ Wepu DNA na-agbakwunyeghị DNA
■ A na-arụ ọrụ niile dị mfe na ọnụ ụlọ okpomọkụ
■ Enwere ike ịrụcha ọrụ ngwa ngwa n'ime nkeji iri atọ
■ Nchekwa-ọ dịghị ihe reagent organic achọrọ
■ Ịdị ọcha dị elu -OD260 / 280≈1.8-2.1

Ngwa ngwa

Ọ dabara maka mmịpụta na ịdị ọcha nke mkpokọta RNA site na anụ ahụ dị ọhụrụ ma ọ bụ oyi kpọnwụrụ dị iche iche ma ọ bụ sel emebere.

Ngwa ngwaahịa

∎ Ngwa mgbada ala: njikọ cDNA nke mbụ, RT-PCR, cloning molecular, Northern Blot, wdg.
■ Ihe nlele: anụ ahụ anụmanụ, mkpụrụ ndụ omenala
■ Usoro onunu ogwu: Tissues 10-20mg, Sel (2-5) × 10⁶
∎ Oke kachasị ike ijikọ DNA nke kọlụm nchacha: 80 μg
■ Olu olu: 50-200 μl

Usoro ọrụ

Eserese

Ngwakọta Mkpokọta RNA nke anụmanụ na-agwọ 20mg
Ihe nlele òké ọhụrụ, were 5% RNA 1% agar sachara ọcha

Glycogel electrophoresis
1: Spleen 2: Akụrụ
3: Imeju 4: Obi

Nchekwa na ndụ nchekwa

Enwere ike ịchekwa ngwa ahụ maka ọnwa 24 na okpomọkụ nke ụlọ (15-25 ℃) ma ọ bụ 2-8 ℃ maka ogologo oge.Enwere ike ịchekwa ihe nchekwa RL1 na 4 ℃ maka otu ọnwa ka ịgbakwunye β-mercaptoethanol (nhọrọ).

Iji bụrụ nsonaazụ nke ọkachamara na nrụzi nke anyị, ụlọ ọrụ anyị enwetala aha ọma n'etiti ndị ahịa niile gburugburu ụwa maka 2019 China New Design China 32 Well Plate Magnetic Bead Method Rna Nucleic Acid Extraction Purification Isolation Kit maka Extractor na FDA kwadoro, ebumnuche nke ụlọ ọrụ anyị bụ mgbe niile inye onyinye kacha mma na ọnụahịa kacha mma.Anyị na-achọ n'ihu ime ụlọ ọrụ gị!
2019 China New DesignChina Viral Nucleic Acid iche, Ngwọta mmịpụta DNA Rna, Anyị na-na-anọgidesi ike na azụmahịa kacha mkpa “Quality First, Ịsọpụrụ nkwekọrịta na guzo site aha ọma, na-enye ndị ahịa na-eju afọ ngwaahịa na ọrụ.” A na-anabata ndị enyi ma ndị nọ n’ụlọ ma ná mba ọzọ ka anyị na ha na-enwe mmekọrịta achụmnta ego ebighị ebi.