Ndị na-emepụta China maka 2 × Concentrated Premix maka Sample na-adịghị ọcha Real-Time Quantitative PCR Direct Multiplex Probe Qpcr Mix Plus U
The Organization keeps on the process concept “scientific management, high quality and efficiency primacy, purchaser supreme for China Manufacturer for 2 × Concentrated Premix for Unpurified Sample Real-Time Quantitative PCR Direct Multiplex Probe Qpcr Mix Plus U, Our Business is dedicated to giving customers with significant and secure top quality products with any agressive price, biko na-eme ka ahịa anyị dị elu na ọnụ ahịa ahịa ọ bụla.
Nzukọ a na-agbaso usoro a echiche "njikwa sayensị, ịdị elu dị elu na ịdị mma nke ọma, onye na-azụ ihe kachasị maka.China Taq DNA Polymerase na Qpcr, Ụlọ ọrụ anyị nwere ike dị ukwuu ma nwee usoro netwọk ahịa na-akwụsi ike na nke zuru oke.Anyị na-achọ ka anyị na ndị ahịa niile si n'ụlọ na mba ọzọ guzobe mmekọrịta azụmahịa dị mma na-adabere na uru otu.
Real Time PCR primer ụkpụrụ imewe
Na-aga n'ihu Primer na Reverse Primer
Maka Real Time PCR, imewe primer dị ezigbo mkpa.Primers metụtara nkọwapụta na arụmọrụ nke nkwalite PCR, enwere ike hazie ya na ntụnyere ụkpụrụ ndị a:
- Ogologo oge mbụ: 18-30bp.
- Ọdịnaya GC: 40-60%.
- Uru Tm: Akụrụngwa imewe nke mbụ, dị ka Primer 5, nwere ike inye uru Tm nke primer.Ụkpụrụ Tm nke ndị na-ebuli elu na nke mgbada kwesịrị ịdị nso dịka o kwere mee.A nwekwara ike iji usoro mgbako Tm: Tm = 4 °C (G + C) + 2 Celsius C (A + T).Mgbe ị na-arụ PCR, a na-ahọrọ okpomọkụ dị n'okpuru uru Tm primer nke 5 Celsius dị ka okpomọkụ na-ekpo ọkụ (mmụba kwekọrọ na okpomọkụ na-ekpo ọkụ nwere ike ịbawanye nkọwa nke mmeghachi omume PCR).
- Ngwaahịa mbụ na PCR:
- Design primer PCR amplification ogologo ngwaahịa bụ ọkachamma 100-150bp.
- Ekwesịrị ịzere primers imewe na mpaghara nhazi nke abụọ nke ndebiri dị ka o kwere mee.
- Zenarị nhazi ntọala 2 ma ọ bụ karịa n'etiti nsọtụ 3 nke elu na ala ala.
- Primer 3′ terminal base enweghị ike ịnọ ya na 3 mgbakwunye G ma ọ bụ C.
- Ndị primers n'onwe ha enweghị ike ịnwe ihe mgbakwunye, ma ọ bụghị ya, a ga-emepụta usoro ntutu isi, na-emetụta mmụba PCR.
- Ekwesịrị kesaa ATCG n'otu n'otu dịka o kwere mee n'usoro nke mbụ, yana ebe njedebe 3′ kwesịrị izere ka T.
Ihe mgbakwunye1:Cebe DirectRT-qPCR Ngwakọta ihet mgbakwunye mkpọ
1.Cell Lysis Solution
| Cell Lysis Solution | |||
| Ngwa ngwa (24-well lysis sistem / ọma) | DRT-01011-A1 | DRT-01011-A2 | |
| 100 T | 500 T | ||
| AkụkụI | Ihe nchekwa CL | 20 ml | 100 ml |
| Foregene Protease Plus II | 400 μl | 1 ml × 2 | |
| Ihe nchekwa ST | 1 ml × 2 | 10 ml | |
| AkụkụII | Nchicha DNA | 400 μl | 1 ml × 2 |
| Ngwakọta RT | |
| Ngwa ngwa (Sistemụ mmeghachi omume 20 μl) | DRT-01011-B1 |
| 200 T | |
| Ngwakọta 5 × Direct RT Mix | 800 μl |
| ddH na-efu RNase2O | 1.7 ml × 2 |
| Ngwakọta qPCR | ||
| Ngwa ngwa (Sistemụ mmeghachi omume 20 μl) | DRT-01021-C1 | DRT-01021-C2 |
| 200 T | 1000 T | |
| 2 × Direct qPCR Mix-Taqman | 1 ml × 2 | 1.7 ml × 6 |
| 20× ROX ntụaka agbaji | 40 μl | 200 μl |
| ddH na-efu RNase2O | 1.7 ml | 10 ml |
The Organization keeps on the process concept “scientific management, high quality and efficiency primacy, purchaser supreme for China Manufacturer for 2 × Concentrated Premix for Unpurified Sample Real-Time Quantitative PCR Direct Multiplex Probe Qpcr Mix Plus U, Our Business is dedicated to giving customers with significant and secure top quality products with any agressive price, biko na-eme ka ahịa anyị dị elu na ọnụ ahịa ahịa ọ bụla.
Onye nrụpụta China makaChina Taq DNA Polymerase na Qpcr, Ụlọ ọrụ anyị nwere ike dị ukwuu ma nwee usoro netwọk ahịa na-akwụsi ike na nke zuru oke.Anyị na-achọ ka anyị na ndị ahịa niile si n'ụlọ na mba ọzọ guzobe mmekọrịta azụmahịa dị mma na-adabere na uru otu.
Real Time PCR primer ụkpụrụ imewe
Na-aga n'ihu Primer na Reverse Primer
Maka Real Time PCR, imewe primer dị ezigbo mkpa.Primers metụtara nkọwapụta na arụmọrụ nke nkwalite PCR, enwere ike hazie ya na ntụnyere ụkpụrụ ndị a:
- Ogologo oge mbụ: 18-30bp.
- Ọdịnaya GC: 40-60%.
- Uru Tm: Akụrụngwa imewe nke mbụ, dị ka Primer 5, nwere ike inye uru Tm nke primer.Ụkpụrụ Tm nke ndị na-ebuli elu na nke mgbada kwesịrị ịdị nso dịka o kwere mee.A nwekwara ike iji usoro mgbako Tm: Tm = 4 °C (G + C) + 2 Celsius C (A + T).Mgbe ị na-arụ PCR, a na-ahọrọ okpomọkụ dị n'okpuru uru Tm primer nke 5 Celsius dị ka okpomọkụ na-ekpo ọkụ (mmụba kwekọrọ na okpomọkụ na-ekpo ọkụ nwere ike ịbawanye nkọwa nke mmeghachi omume PCR).
- Ngwaahịa mbụ na PCR:
- Design primer PCR amplification ogologo ngwaahịa bụ ọkachamma 100-150bp.
- Ekwesịrị ịzere primers imewe na mpaghara nhazi nke abụọ nke ndebiri dị ka o kwere mee.
- Zenarị nhazi ntọala 2 ma ọ bụ karịa n'etiti nsọtụ 3 nke elu na ala ala.
- Primer 3′ terminal base enweghị ike ịnọ ya na 3 mgbakwunye G ma ọ bụ C.
- Ndị primers n'onwe ha enweghị ike ịnwe ihe mgbakwunye, ma ọ bụghị ya, a ga-emepụta usoro ntutu isi, na-emetụta mmụba PCR.
- Ekwesịrị kesaa ATCG n'otu n'otu dịka o kwere mee n'usoro nke mbụ, yana ebe njedebe 3′ kwesịrị izere ka T.
Ihe mgbakwunye1:Cebe DirectRT-qPCR Ngwakọta ihet mgbakwunye mkpọ
1.Cell Lysis Solution
| Cell Lysis Solution | |||
| Ngwa ngwa (24-well lysis sistem / ọma) | DRT-01011-A1 | DRT-01011-A2 | |
| 100 T | 500 T | ||
| AkụkụI | Ihe nchekwa CL | 20 ml | 100 ml |
| Foregene Protease Plus II | 400 μl | 1 ml × 2 | |
| Ihe nchekwa ST | 1 ml × 2 | 10 ml | |
| AkụkụII | Nchicha DNA | 400 μl | 1 ml × 2 |
| Ngwakọta RT | |
| Ngwa ngwa (Sistemụ mmeghachi omume 20 μl) | DRT-01011-B1 |
| 200 T | |
| Ngwakọta 5 × Direct RT Mix | 800 μl |
| ddH na-efu RNase2O | 1.7 ml × 2 |
| Ngwakọta qPCR | ||
| Ngwa ngwa (Sistemụ mmeghachi omume 20 μl) | DRT-01021-C1 | DRT-01021-C2 |
| 200 T | 1000 T | |
| 2 × Direct qPCR Mix-Taqman | 1 ml × 2 | 1.7 ml × 6 |
| 20× ROX ntụaka agbaji | 40 μl | 200 μl |
| ddH na-efu RNase2O | 1.7 ml | 10 ml |
Akwụkwọ ntuziaka:
