Kedu nke nwere usoro nchịkwa sayensị zuru oke nke nkà mmụta sayensị, àgwà magburu onwe ya na ezigbo okpukpe, anyị na-enweta aha ọma ma nọrọ ebe a maka China New Product China Baypure Wholesales Tissue Genomic Nucleic Acid Extraction Kit Reagents Asia Market, Mgbe ị na-eji tenet nke "okwukwe dabeere, onye ahịa mbụ", anyị na-anabata ndị ahịa na ekwentị ma ọ bụ e-mail anyị maka imekọ ihe ọnụ.
Nke nwere usoro njikwa sayensị zuru oke, ezigbo mma na okpukperechi dị mma, anyị na-enweta aha ọma ma were mpaghara a makaAkpaaka Nucleic Acid Extractor Cleanification, Ngwa China Tissue Genomic DNA Kit, A na-ebupụ ngwaahịa anyị n'ụwa niile.Ndị ahịa anyị na-enwe afọ ojuju mgbe niile na àgwà anyị nwere ike ịdabere na ya, ọrụ ndị ahịa na-adabere na ọnụ ahịa asọmpi.Ebumnuche anyị bụ "ịga n'ihu na-enweta nkwado gị site n'ịrara mbọ anyị na-emeziwanye ngwaahịa na ọrụ anyị mgbe niile iji hụ na afọ ojuju nke ndị ọrụ njedebe anyị, ndị ahịa, ndị ọrụ, ndị na-ebubata ya na obodo ụwa niile anyị na-arụkọ ọrụ."

Nkọwa ngwa ngwa

Nkwadebe 50, 200 Preps

Ngwa a na-eji kọlụm spine na usoro nke ụlọ ọrụ anyị mepụtara, nke nwere ike wepụ ịdị ọcha dị elu na nke dị elu RNA site na anụ ahụ dị iche iche nke anụmanụ na-arụ ọrụ dị elu. Ọ na-enye DNA-Cleaning Column na-arụ ọrụ nke ọma, nke nwere ike ikewapụ na adsorb genomic DNA site na supernatant na anụ ahụ lysate, dị mfe na-azọpụta oge;Kọlụm naanị RNA nwere ike jikọta RNA nke ọma ma nwee ike hazie ya n'otu oge site na iji usoro pụrụ iche Ọtụtụ nlele.

Usoro dum bụ RNase-Free, nke mere na RNA amịpụtara adịghị emebi emebi;Buffer RW1, Buffer RW2 sistemu ịsacha ihe, nke mere na RNA enwetara enweghị protein, DNA, ion, na mmetọ ngwakọta organic.

Ngwa ngwa:

Atụmatụ & uru

■ Ọ dịghị mkpa ichegbu onwe gị maka mbibi RNA;Sistemụ niile bụ RNase-Free
∎ Wepu DNA nke ọma site na iji kọlụm na-ehicha DNA
■ Wepu DNA na-agbakwunyeghị DNA
■ A na-arụ ọrụ niile dị mfe na ọnụ ụlọ okpomọkụ
■ Enwere ike ịrụcha ọrụ ngwa ngwa n'ime nkeji iri atọ
■ Nchekwa-ọ dịghị ihe reagent organic achọrọ
■ Ịdị ọcha dị elu -OD260 / 280≈1.8-2.1

Ngwa ngwa

Ọ dabara maka mmịpụta na ịdị ọcha nke mkpokọta RNA site na anụ ahụ dị ọhụrụ ma ọ bụ oyi kpọnwụrụ dị iche iche ma ọ bụ sel emebere.

Ngwa ngwaahịa

∎ Ngwa mgbada ala: njikọ cDNA nke mbụ, RT-PCR, cloning molecular, Northern Blot, wdg.
■ Ihe nlele: anụ ahụ anụmanụ, mkpụrụ ndụ omenala
■ Usoro onunu ogwu: Tissues 10-20mg, Sel (2-5) × 10⁶
∎ Oke kachasị ike ijikọ DNA nke kọlụm nchacha: 80 μg
■ Olu olu: 50-200 μl

Usoro ọrụ

Eserese

Ngwakọta Mkpokọta RNA nke anụmanụ na-agwọ 20mg
Ihe nlele òké ọhụrụ, were 5% RNA 1% agar sachara ọcha

Glycogel electrophoresis
1: Spleen 2: Akụrụ
3: Imeju 4: Obi

Nchekwa na ndụ nchekwa

Enwere ike ịchekwa ngwa ahụ maka ọnwa 24 na okpomọkụ nke ụlọ (15-25 ℃) ma ọ bụ 2-8 ℃ maka ogologo oge.Enwere ike ịchekwa ihe nchekwa RL1 na 4 ℃ maka otu ọnwa ka ịgbakwunye β-mercaptoethanol (nhọrọ).

Kedu nke nwere usoro nchịkwa sayensị zuru oke nke nkà mmụta sayensị, àgwà magburu onwe ya na ezigbo okpukpe, anyị na-enweta aha ọma ma nọrọ ebe a maka China New Product China Baypure Wholesales Tissue Genomic Nucleic Acid Extraction Kit Reagents Asia Market, Mgbe ị na-eji tenet nke "okwukwe dabeere, onye ahịa mbụ", anyị na-anabata ndị ahịa na ekwentị ma ọ bụ e-mail anyị maka imekọ ihe ọnụ.
Ngwaahịa ọhụrụ ChinaNgwa China Tissue Genomic DNA Kit, Akpaaka Nucleic Acid Extractor Cleanification, A na-ebupụ ngwaahịa anyị n'ụwa niile.Ndị ahịa anyị na-enwe afọ ojuju mgbe niile na àgwà anyị nwere ike ịdabere na ya, ọrụ ndị ahịa na-adabere na ọnụ ahịa asọmpi.Ebumnuche anyị bụ "ịga n'ihu na-enweta nkwado gị site n'ịrara mbọ anyị na-emeziwanye ngwaahịa na ọrụ anyị mgbe niile iji hụ na afọ ojuju nke ndị ọrụ njedebe anyị, ndị ahịa, ndị ọrụ, ndị na-ebubata ya na obodo ụwa niile anyị na-arụkọ ọrụ."