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Onye na-eweta China China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit

Onye na-ebu China China Factory Magnetic Bead Acid Kit Nucleic Test Kit Rt-PCR gosiputara onyonyo.
  • Onye na-eweta China China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit
  • Onye na-eweta China China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit
  • Onye na-eweta China China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit
  • Onye na-eweta China China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit

Nkọwa ngwa:

Ngwa a na-eji usoro nchekwa lysis pụrụ iche na-ahapụ ngwa ngwa DNA genomic site na zebrafish na anụ ahụ azụ mmiri ndị ọzọ, ọdụ ọdụ ma ọ bụ nsen azụ maka mmeghachi omume PCR, yabụ na ọ dabara adaba maka nyocha mkpụrụ ndụ buru ibu.Usoro nke ịtọhapụ DNA genomic site na nchekwa lysis na-agwụ n'ime nkeji 10-30 na 65 Celsius.Ọ nweghị usoro ndị ọzọ dị ka protein na mwepụ RNA achọrọ, yana DNA achọpụtara ewepụtara enwere ike iji dị ka ndebiri maka mmeghachi omume PCR.

2× PCR EasyTM Mix (UNG) na-eji dUTP kama dTTP na ndabere nke 2 × PCR EasyTM Mix, ma gbakwunye UNG enzyme (Uracil-N-glycosylase) nke nwere ike imebi template nwere dUTP n'otu oge.Tupu mmeghachi omume PCR, a na-eji enzyme UNG mebie ngwaahịa PCR nwere uracil.Enzyme UNG agaghị enwe mmetụta ọ bụla na ndebiri nke na-enweghị uracil, si otú a na-ahụ na nkọwapụta na izi ezi nke nkwalite na igbochi ohere nke nyocha mkpụrụ ndụ ihe nketa buru ibu.Mmetọ nke ngwaahịa PCR mere.

D-Taq DNA polymerase bụ DNA polymerase nke Foregene mepụtara maka mmeghachi omume PCR kpọmkwem.D-Taq DNA polymerase nwere nnabata siri ike maka ọtụtụ ihe mgbochi mmeghachi omume PCR, ma nwee ike ịbawanye ọnụọgụ DNA nke ọma na sistemụ mmeghachi omume dị mgbagwoju anya, yana ọsọ nkwalite nwere ike iru 2Kb / min, nke dabara adaba maka mmeghachi omume PCR Direct.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Anyị nwere otu ndị na-arụ ọrụ nke ọma iji mesoo ajụjụ sitere n'aka ndị ahịa.Ihe mgbaru ọsọ anyị bụ "100% afọ ojuju ndị ahịa site na àgwà ngwaahịa anyị, ọnụahịa & ọrụ otu anyị" ma nwee aha ọma n'etiti ndị ahịa.Na ọtụtụ ụlọ mmepụta ihe, anyị nwere ike inye a dịgasị iche iche nke China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit, Anyị ike ngwa ngwa toro na size na aha n'ihi na nke ya zuru okè nraranye elu àgwà n'ichepụta, ọkpụrụkpụ price nke ngwọta na kediegwu ahịa ọrụ.
Anyị nwere otu ndị na-arụ ọrụ nke ọma iji mesoo ajụjụ sitere n'aka ndị ahịa.Ihe mgbaru ọsọ anyị bụ "100% afọ ojuju ndị ahịa site na àgwà ngwaahịa anyị, ọnụahịa & ọrụ otu anyị" ma nwee aha ọma n'etiti ndị ahịa.Na ọtụtụ ụlọ mmepụta ihe, anyị nwere ike inye a dịgasị iche iche nkeNgwa Mwepụta nke China, Ngwa Nwepụta Acid Nucleic, Ụlọ ọrụ anyị na-etinye uche mgbe niile na mmepe nke ahịa mba ụwa.Anyị nwere ugbu a ọtụtụ ndị ahịa na Russia, mba Europe, USA, mba Middle East na mba Africa.Anyị na-agbaso àgwà ahụ mgbe niile mgbe ọrụ na-ekwe nkwa izute ndị ahịa niile.

Nkọwapụta

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ngwa ngwa

Akụkụ I

Ihe nchekwa FP

Protease nke mbụ

Ihe nchekwa 6 × DNA na-ebufe

Akụkụ II

2 × PCR dị mfeTMNgwakọta (UNG)

Ntuziaka

Atụmatụ & uru

■ Ọnweghị ihe na-ewe oge na ọnụ ahịa DNA dị ọcha achọrọ.

■ Ihe nlele achọrọ dị ntakịrị, ọ bụ ezie na enwere ike ịnwale 1mg azụ caudal fin ma ọ bụ akwa azụ 10.

∎ Ọ dịghị ọgwụgwọ pụrụ iche dị ka egweri na ịzọpịa ihe a chọrọ, ma ọrụ ahụ dị mfe.

∎ Sistemụ PCR na-egbochi mmetọ 2 × PCR EasyTM Mix (UNG), nke nwere ike iwepụ mmetọ nke ngwaahịa PCR nke ọma wee hụ na nkọwapụta na izi ezi nke nkwalite.

∎ Sistemu PCR kachasị mma na-eme ka PCR nwee nkọwa dị elu yana nnabata siri ike nye ndị na-egbochi mmeghachi omume PCR.

Ngwa ngwa

Ngwa: azụ zebra na azụ mmiri ndị ọzọ.

DNA ewepụtara na lysis sample: naanị ejiri ya dị ka ndebiri PCR.

Enwere ike iji ngwa ahụ maka ebumnuche ndị a: njirimara transgenosis, genotyping anụmanụ, wdg.

Nchekwa na Shelf ndụ

Ekwesịrị ịchekwa akụkụ I nke ngwa a na 2-8 ℃.

Enwere ike ịchekwa reagent Buffer FP maka ọnwa 12 n'okpuru ọnọdụ akọrọ;Enwere ike ịchekwa ya na 2-8 ℃ maka nchekwa ogologo oge.

v Reagent Foregene Protease nwere usoro pụrụ iche.Iji hụ na ọrụ ya na nkwụsi ike ya, biko chekwaa ya na 4 ℃ maka ọnwa 12.

Enwere ike ịchekwa ihe nchekwa vReagent 6 × DNA na 4℃ ma ọ bụ -20℃ ruo ogologo oge.

Ekwesịrị ịchekwa akụkụ II nke ngwa a na -20 ℃.

vReagent 2×PCR EasyTM Mix(UNG), ọ bụrụ na-eji ya ugboro ugboro, ọ nwekwara ike ịchekwa na 4℃ maka obere oge nchekwa (jiri elu n'ime 10 ụbọchị).

Anyị nwere otu ndị na-arụ ọrụ nke ọma iji mesoo ajụjụ sitere n'aka ndị ahịa.Ihe mgbaru ọsọ anyị bụ "100% afọ ojuju ndị ahịa site na àgwà ngwaahịa anyị, ọnụahịa & ọrụ otu anyị" ma nwee aha ọma n'etiti ndị ahịa.Na ọtụtụ ụlọ mmepụta ihe, anyị nwere ike inye a dịgasị iche iche nke China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit, Anyị ike ngwa ngwa toro na size na aha n'ihi na nke ya zuru okè nraranye elu àgwà n'ichepụta, ọkpụrụkpụ price nke ngwọta na kediegwu ahịa ọrụ.
Onye na-eweta ChinaNgwa Mwepụta nke China, Ngwa Nwepụta Acid Nucleic, Ụlọ ọrụ anyị na-etinye uche mgbe niile na mmepe nke ahịa mba ụwa.Anyị nwere ugbu a ọtụtụ ndị ahịa na Russia, mba Europe, USA, mba Middle East na mba Africa.Anyị na-agbaso àgwà ahụ mgbe niile mgbe ọrụ na-ekwe nkwa izute ndị ahịa niile.

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