Ọnụ ego n'ofe China n'efu Sample karịa ọnụ ahịa karịa Wondfo 32t 64t DNA Rna Nucleic Acid Purification Reagent Kit Site na Onye nrụpụta mbụ nwere CE na FDA
Nkọwa ngwa:
RNase-efu
Na-arụ ọrụ na ụlọ okpomọkụ (15-25 ℃)
Site na kọlụm na-ehicha DNA
Mkpụrụ RNA dị elu
Ngwa ngwa: Mechaa mmịpụta n'ime nkeji iri na otu
Nchekwa: Ọ nweghị kemịkalụ Organic
Ezi àgwà na-abịa na-amalite na;ọrụ bụ nke mbụ;nzukọ bụ imekọ ihe ọnụ" bụ anyị Enterprise nkà ihe ọmụma nke a na-ahụ anya mgbe niile na-achụ anyị ike maka Discount wholesale China Free Sample More Cost-Effective Than Wondfo 32t 64t DNA Rna Nucleic Acid Purification Reagent Kit From Original Manufacturer with CE na FDA, Anyị ga-anọgide na-arụ ọrụ ike na dị ka anyị na-atụle anyị kacha mma na-enye ndị kasị dị irè ahịa na-ere ahịa na-ere ahịa na-ere ahịa ọ bụla na-ere ahịa na-ere ahịa na-ere ahịa na-ere ahịa na-ere ahịa ọ bụla.afọ ojuju gị, ebube anyị!!!
Ezi àgwà na-abịa na-amalite na;ọrụ bụ nke mbụ;nzukọ bụ imekọ ihe ọnụ" bụ nkà ihe ọmụma nke ụlọ ọrụ anyị nke a na-ahụ anya ma na-achụso ụlọ ọrụ anyị makaChina Nucleic Acid nkewa Reagent, Nucleic Acid Isolating Reagent, Anyị na-enweta ihe ngwọta kasị mma na ọkachamara ahịa na teknụzụ team.With mmepe nke anyị ụlọ ọrụ, anyị na-enwe ike na-anapụta ndị ahịa kasị mma ngwaahịa, ezi oru nkwado, zuru okè mgbe-sales ọrụ.
Nkọwa
Ngwa a na-eji kọlụm ogho na usoro nke Foregene mepụtara, nke nwere ike wepụta RNA dị ọcha na ịdị elu nke ọma site na mkpụrụ ndụ emebere na efere 96, 24, 12 na 6.
Ngwa ahụ na-enye kọlụm DNA-Cleaning nke ọma, nke nwere ike kewapụ ihe dị elu na cell lysate ngwa ngwa, kechie ma wepụ DNA genomic.Ọrụ ahụ dị mfe ma na-azọpụta oge.
Kọlụm naanị RNA nwere ike jikọta RNA nke ọma yana usoro pụrụ iche.Enwere ike ịhazi ọnụ ọgụgụ dị ukwuu nke ihe nlele n'otu oge.
Ngwa ngwa
| Ngwa mejupụtara | RE-03111 | RE-03114 |
| 50 T | 200 T | |
| Ihe nchekwa cRL1* | 25 ml | 100 ml |
| Ihe nchekwa cRL2 | 15 ml | 60 ml |
| Ihe nchekwa RW1* | 25 ml | 100 ml |
| Ihe nchekwa RW2 | 24 ml | 96 ml |
| ddH2O na-efu RNase | 10 ml | 40 ml |
| RNA-naanị kọlụm | 50 | 200 |
| Kọlụm na-ehicha DNA | 50 | 200 |
| Ntuziaka | 1 | 1 |
*Biko yi gloves ma were usoro nchebe n'oge a na-arụ ọrụ dịka Buffer cRL1 na Buffer RW1 nwere nnu chaotropic na-akpasu iwe.
Atụmatụ & uru
■ A na-arụ ọrụ ahụ dum na ụlọ okpomọkụ (15-25 ℃), na-enweghị ice bat na obere okpomọkụ centrifugation.
■ Ngwa niile enweghị RNase, ọ nweghị mkpa ichegbu onwe gị maka mmebi RNA.
∎ kọlụm DNA- Cleaning na-ejikọta DNA kpọmkwem, ka ngwa ahụ wee nwee ike wepụ nje DNA nje na-etinyeghị DNA ọzọ.
∎ Mkpụrụ RNA dị elu: Kọlụm naanị RNA nwere ike sachaa RNA nke ọma.
∎ Ọsọ ọsọ: dị mfe iji rụọ ọrụ ma nwee ike mechaa n'ime nkeji iri na otu.
■ Nchekwa: Ọ dịghị ihe reagent organic achọghị.
■ Ọdịmma dị elu: RNA dị ọcha dị oke ọcha, enweghị protein na adịghị ọcha ndị ọzọ, ọ nwekwara ike izute nnwale dị iche iche na-esote.
Ngwa ngwa
Ọ dabara maka mmịpụta na ịsacha mkpokọta RNA site na mkpụrụ ndụ emebere na efere 96, 24, 12 na 6.
Usoro ọrụ
Eserese
Eserese batrị agarose gel nke Cell Total RNA Isolation Kit na-emeso ọnụọgụ mkpụrụ ndụ dị iche iche dị n'elu, elu 20μl, were 2μl dị ọcha RNA 1%.
Nchekwa na ndụ nchekwa
Enwere ike ịchekwa ngwa ahụ maka ọnwa 12 na ụlọ okpomọkụ (15-25 ℃) ma ọ bụ 2-8 ℃ maka ogologo oge (ọnwa 24).
Buffer cRL1 nwere ike ịchekwa na 4 ℃ maka 1 ọnwa mgbe agbakwunyere 2-hydroxy-1-ethanethiol (nhọrọ) .Ezi àgwà na-abịa na-amalite na;ọrụ bụ nke mbụ;nzukọ bụ imekọ ihe ọnụ" bụ anyị Enterprise nkà ihe ọmụma nke a na-ahụ anya mgbe niile na-achụ anyị ike maka Discount wholesale China Free Sample More Cost-Effective Than Wondfo 32t 64t DNA Rna Nucleic Acid Purification Reagent Kit From Original Manufacturer with CE na FDA, Anyị ga-anọgide na-arụ ọrụ ike na dị ka anyị na-atụle anyị kacha mma na-enye ndị kasị dị irè ahịa na-ere ahịa na-ere ahịa na-ere ahịa ọ bụla na-ere ahịa na-ere ahịa na-ere ahịa na-ere ahịa na-ere ahịa ọ bụla.afọ ojuju gị, ebube anyị!!!
Ọnụ ego n'ogbeChina Nucleic Acid nkewa Reagent, Nucleic Acid Isolating Reagent, Anyị na-enweta ihe ngwọta kasị mma na ọkachamara ahịa na teknụzụ team.With mmepe nke anyị ụlọ ọrụ, anyị na-enwe ike na-anapụta ndị ahịa kasị mma ngwaahịa, ezi oru nkwado, zuru okè mgbe-sales ọrụ.
A naghị ewepụta RNA ma ọ bụ RNA dị ala
A na-enwekarị ihe dị iche iche na-emetụta arụmọrụ mgbake, dịka: ọdịnaya RNA anụ ahụ, usoro ọrụ, elution volume, wdg.
1. Ice bath ma ọ bụ cryogenic (4 Celsius C) centrifugation mere n'oge ọrụ.
Nkwanye: Na-arụ ọrụ na ụlọ okpomọkụ (15-25 Celsius C) n'ime usoro dum, emela ice bat na centrifuge na obere okpomọkụ.
2. Nchekwa nlele na-ezighi ezi ma ọ bụ oge nchekwa oke.
Nkwanye: Chekwaa ihe nlele na -80 Celsius ma ọ bụ ifriizi na mmiri mmiri nitrogen ma zere iji friza eme ihe ugboro ugboro;gbalịa iji anụ ahụ ọhụrụ ma ọ bụ mkpụrụ ndụ emebere maka mmịpụta RNA.
3. ezughị ezu sample lysis.
Nkwanye: Mgbe ị na-eme ka anụ ahụ dị n'otu, hụ na anụ ahụ na-emekọ ihe nke ọma yana na mkpụrụ ndụ anụ ahụ kewara nke ọma iji kọwaa ntọhapụ nke RNA.
4. A naghị atụkwasị eluent nke ọma.
Nkwanye: Kwenye na ddH enweghị RNase2A na-agbakwunye O n'ime ala n'etiti akpụkpọ ahụ kọlụm nke ịdị ọcha.
5. Agbakwunyeghị olu ziri ezi nke ethanol zuru oke na Buffer RL2 ma ọ bụ Buffer RW2.
Nkwanye: Soro ntuziaka a, tinye nha ziri ezi nke ethanol zuru oke na Buffer RL2 na Buffer RW2 wee gwakọta nke ọma tupu i jiri ngwa ahụ.
6. Usoro ihe nlele anụ ahụ adịghị mma.
Nkwanye: Jiri 10-20 mg nke anụ ahụ ma ọ bụ (1-5) × 106mkpụrụ ndụ kwa 500 μl ihe nchekwa RL1, n'ihi na iji anụ ahụ gabigara ókè nwere ike ime ka mmịpụta RNA belata.
7. Olu elution na-ezighi ezi ma ọ bụ elution ezughị ezu.
Nkwanye: Oke elution nke kọlụm nchacha bụ 50-200 μl;Ọ bụrụ na mmetụta elution adịghị afọ ojuju, a na-atụ aro ịgbatị oge ntinye ụlọ okpomọkụ mgbe agbakwunyere ddH na-enweghị ọkụ RNase.2O, ọmụmaatụ maka nkeji 5-10.
8.The kọlụm dị ọcha nwere ethanol fọdụrụnụ mgbe Buffer RW2 saa.
Nkwanye: Ọ bụrụ na e nwere ihe fọdụrụ na ethanol mgbe ịsachara Buffer RW2, centrifugation tube efu maka 1min, enwere ike ịbawanye oge ọrụ centrifugation tube efu ruo 2min, ma ọ bụ enwere ike idobe kọlụm dị ọcha na ụlọ okpomọkụ maka 5 min iji wepụ ethanol fọdụrụnụ nke ọma.
RNA dị ọcha na-eweda ya ala
Àgwà nke RNA a sachara ejikọta na ihe ndị dị ka ichekwa ihe nlele, mmetọ RNase, na nhazi, wdg.
1. A naghị edebe ihe nlele anụ ahụ n'oge.
Nkwanye: Ọ bụrụ na ejighị ihe nlele anụ ahụ ma ọ bụ sel mee ihe n'oge kwesịrị ekwesị mgbe nchịkọtachara, ozugbo cryopreserve na -80 Celsius C ma ọ bụ nitrogen mmiri mmiri.Iji wepụ RNA, jiri anụ ahụ ma ọ bụ ihe nlele cell ewepụtara ọhụrụ mgbe ọ bụla enwere ike.
2. Ugboro ugboro ifriizi-thawing nke anụ ahụ sample.
Nkwanye: Mgbe ị na-echekwa ihe nlele anụ ahụ, ọ kacha mma ịkpụ ya n'ime obere iberibe maka nchekwa, ma wepụ otu n'ime iberibe ahụ mgbe ị na-eji ha ka ịzenarị oyiri ugboro ugboro nke ihe nlele ahụ na mmebi nke RNA.
3. A na-ewebata RNase ma ọ bụ ghara iyi uwe mkpuchi, ihe mkpuchi, wdg n'oge ọrụ ahụ.
Nkwanye: A na-eme nnwale mmịpụta RNA kacha mma n'ime ụlọ njikwa RNA dị iche iche wee kpochapụ tebụl tupu nnwale ahụ.
Yiri gloves na masks enwere ike iwepụ n'oge nnwale ahụ iji belata mmebi RNA kpatara site na iwebata RNase.
4. A na-eji RNase metọọ reagents n'oge eji.
Nkwanye: Dochie jiri ngwa Mkpokọta anụ ọhịa RNA ọhụrụ maka nnwale ndị metụtara ya.
5. Ọkpụkpọ centrifuge, ndụmọdụ, wdg eji eme ihe na RNA na-emetọ ya na RNase.
Nkwanye: Kwenye na tubes centrifuge, ndụmọdụ, pipettes, wdg. ejiri na mmịpụta RNA bụ RNase niile.
RNA emepụtara dị ọcha na-emetụta nnwale ndị dị n'okpuru ala
RNA sachara site na kọlụm dị ọcha, ọ bụrụ na ion nnu, ọdịnaya protein buru ibu ga-emetụta nnwale nke ala, dị ka: ntụgharị ntụgharị, Northern Blot et al.
1. RNA nke elutioned nwere residues ion nnu.
Nkwanye: Kwenye na agbakwunyere oke ethanol ziri ezi na Buffer RW2 ma mee 2 kọlụm nchacha ọcha na ọsọ centrifugal nke egosiri maka ịrụ ọrụ;Ọ bụrụ na enwere ihe fọdụrụ ion nnu, hapụ kọlụm nke ịdị ọcha na Buffer RW2 maka 5 min na ụlọ okpomọkụ ma mee centrifugation iji bulie mwepụ nke mmetọ nnu.
2. Ethanol fọdụrụnụ na elutioned RNA.
Nkwanye: Kwenye na mgbe ịsachara ihe nchekwa RW2, rụọ ọrụ centrifugation tube efu na ọsọ centrifugation egosiputara maka ịrụ ọrụ, mụbaa oge ọrụ centrifugation tube efu ruo 2 min ma ọ bụrụ na ọ ka nwere ihe fọdụrụ na ethanol, ma ọ bụ hapụ ya na ụlọ okpomọkụ maka 5 m.
